FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4377022658> ?p ?o ?g. }

• W4377022658 abstract "Abstract Background Cantharidin (CTD) is a major ingredient of cantharis ( Mylabris phalerata Pallas) and has been used extensively in traditional Chinese medicines. It has been shown to exhibit anticancer activity in multiple types of cancer, especially hepatocellular carcinoma (HCC). However, there is no systematic study on the relationships among the regulatory networks of its targets in HCC therapy. We focused on histone epigenetic regulation and the influence of CTD on the immune response in HCC. Methods We performed a comprehensive analysis of novel CTD targets in HCC based on network pharmacology and RNA-seq approaches. The mRNA levels of target genes were analyzed by qRT-PCR, and the corresponding protein levels were confirmed using enzyme-linked immunosorbent assay (ELISA) and immunohistochemical staining (IHC). ChIP-seq data were visualized by IGV software. The associations of gene transcript levels with the cancer immune score and infiltration level were investigated using TIMER. In vivo, the H22 mouse model of hepatocellular carcinoma was established by treatment with CTD and 5-Fu. The immune cell proportions in the blood were elevated in model mice, as shown by flow cytometry. Results We identified 58 targets of CTD, which were involved in various pathways in cancer, including apoptosis, the cell cycle, EMT and immune pathways. Moreover, we found that 100 EMT-related genes were differentially expressed after CTD treatment in HCC cells. Interestingly, our results confirmed that the EZH2/H3K27me3 -related cell cycle pathway is a therapeutic target of CTD in antitumour. In addition, we evaluated the influence of CTD on the immune response. Our data showed that the significantly enriched gene sets were positively correlated with the chemokine biosynthetic and chemokine metabolic modules. The proportions of CD4+/CD8 + T cells and B cells were increased, but the proportion of Tregs was decreased after treatment with CTD in vivo. Moreover, we found that the expression of the inflammatory factor and immune checkpoint genes PD­1/PD-L1 was significantly reduced in the mouse model. Conclusion We performed a novel integrated analysis of the potential role of CTD in HCC treatment. Our results provide innovative insight into the mechanism by which cantharidin exerts antitumour effects by regulating target genes expression to mediate apoptosis, EMT, cell cycle progression and the immune response in HCC. Based on the effect of CTD on the immune response, it can be used as a potential effective drug to activate antitumour immunity for the treatment of liver cancer." @default.
• W4377022658 created "2023-05-19" @default.
• W4377022658 creator A5000005088 @default.
• W4377022658 creator A5000324385 @default.
• W4377022658 creator A5002867372 @default.
• W4377022658 creator A5013389193 @default.
• W4377022658 creator A5024873047 @default.
• W4377022658 creator A5040081599 @default.
• W4377022658 creator A5047942514 @default.
• W4377022658 date "2023-05-18" @default.
• W4377022658 modified "2023-10-06" @default.
• W4377022658 title "Cantharidin suppresses hepatocellular carcinoma development by regulating EZH2/H3K27me3-dependent cell cycle progression and antitumour immune response" @default.
• W4377022658 cites W2148639438 @default.
• W4377022658 cites W2159482845 @default.
• W4377022658 cites W2768326446 @default.
• W4377022658 cites W2774716788 @default.
• W4377022658 cites W2791832446 @default.
• W4377022658 cites W2796006438 @default.
• W4377022658 cites W2803901263 @default.
• W4377022658 cites W2898314774 @default.
• W4377022658 cites W2902844785 @default.
• W4377022658 cites W2976235020 @default.
• W4377022658 cites W2988956091 @default.
• W4377022658 cites W2998924171 @default.
• W4377022658 cites W3024568857 @default.
• W4377022658 cites W3038110038 @default.
• W4377022658 cites W3042664166 @default.
• W4377022658 cites W3089069212 @default.
• W4377022658 cites W3093027838 @default.
• W4377022658 cites W3128646645 @default.
• W4377022658 cites W3134553072 @default.
• W4377022658 cites W3155241267 @default.
• W4377022658 cites W3155637606 @default.
• W4377022658 cites W3171870984 @default.
• W4377022658 cites W3183393571 @default.
• W4377022658 cites W3199802615 @default.
• W4377022658 cites W3199839719 @default.
• W4377022658 cites W3200445508 @default.
• W4377022658 cites W3203289704 @default.
• W4377022658 cites W3205337639 @default.
• W4377022658 cites W3215541436 @default.
• W4377022658 cites W4220684965 @default.
• W4377022658 cites W4220792405 @default.
• W4377022658 cites W4223468143 @default.
• W4377022658 cites W4228999747 @default.
• W4377022658 cites W4281696633 @default.
• W4377022658 cites W4281788501 @default.
• W4377022658 cites W4285038902 @default.
• W4377022658 cites W4294216483 @default.
• W4377022658 cites W4295575616 @default.
• W4377022658 cites W4307425304 @default.
• W4377022658 cites W4307848132 @default.
• W4377022658 cites W4311873367 @default.
• W4377022658 cites W4313327647 @default.
• W4377022658 doi "https://doi.org/10.1186/s12906-023-03975-0" @default.
• W4377022658 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37202806" @default.
• W4377022658 hasPublicationYear "2023" @default.
• W4377022658 type Work @default.
• W4377022658 citedByCount "0" @default.
• W4377022658 crossrefType "journal-article" @default.
• W4377022658 hasAuthorship W4377022658A5000005088 @default.
• W4377022658 hasAuthorship W4377022658A5000324385 @default.
• W4377022658 hasAuthorship W4377022658A5002867372 @default.
• W4377022658 hasAuthorship W4377022658A5013389193 @default.
• W4377022658 hasAuthorship W4377022658A5024873047 @default.
• W4377022658 hasAuthorship W4377022658A5040081599 @default.
• W4377022658 hasAuthorship W4377022658A5047942514 @default.
• W4377022658 hasBestOaLocation W43770226581 @default.
• W4377022658 hasConcept C104317684 @default.
• W4377022658 hasConcept C111368507 @default.
• W4377022658 hasConcept C121608353 @default.
• W4377022658 hasConcept C125533998 @default.
• W4377022658 hasConcept C127313418 @default.
• W4377022658 hasConcept C203014093 @default.
• W4377022658 hasConcept C2778019345 @default.
• W4377022658 hasConcept C29537977 @default.
• W4377022658 hasConcept C41091548 @default.
• W4377022658 hasConcept C50171091 @default.
• W4377022658 hasConcept C502942594 @default.
• W4377022658 hasConcept C54355233 @default.
• W4377022658 hasConcept C553184892 @default.
• W4377022658 hasConcept C55493867 @default.
• W4377022658 hasConcept C86803240 @default.
• W4377022658 hasConcept C8891405 @default.
• W4377022658 hasConceptScore W4377022658C104317684 @default.
• W4377022658 hasConceptScore W4377022658C111368507 @default.
• W4377022658 hasConceptScore W4377022658C121608353 @default.
• W4377022658 hasConceptScore W4377022658C125533998 @default.
• W4377022658 hasConceptScore W4377022658C127313418 @default.
• W4377022658 hasConceptScore W4377022658C203014093 @default.
• W4377022658 hasConceptScore W4377022658C2778019345 @default.
• W4377022658 hasConceptScore W4377022658C29537977 @default.
• W4377022658 hasConceptScore W4377022658C41091548 @default.
• W4377022658 hasConceptScore W4377022658C50171091 @default.
• W4377022658 hasConceptScore W4377022658C502942594 @default.
• W4377022658 hasConceptScore W4377022658C54355233 @default.
• W4377022658 hasConceptScore W4377022658C553184892 @default.
• W4377022658 hasConceptScore W4377022658C55493867 @default.
• W4377022658 hasConceptScore W4377022658C86803240 @default.
• W4377022658 hasConceptScore W4377022658C8891405 @default.

• next