FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4377029079> ?p ?o ?g. }

• W4377029079 abstract "<b>Abstract ID 18095</b> <b>Poster Board 38</b> <b>Aims:</b> Kirsten Rat Sarcoma virus (KRAS) oncogene, found in 20-25% of lung cancer patients, regulates metabolic reprogramming and redox status during tumorigenesis. Recently, histone deacetylase (HDAC) inhibitors are widely investigated for the treatment of KRAS-mutant lung cancer. In the current study, we investigate the effect of HDAC inhibitor (HDACi) belinostat on nuclear factor erythroid 2-related factor 2 (NRF2) and mitochondrial metabolism for the treatment of KRAS-mutant cell lung cancer. <b>Main methods:</b> LC-MS metabolomic study of belinostat on mitochondrial metabolism was performed in KRAS-mutant non-small cell lung cancer cells. Furthermore, L-methionine (methyl-¹³C) isotope tracer was used to explore the effect of the belinostat in one carbon metabolism. Bioinformatic analyses were performed with the metabolomic data to identify the pattern of the significantly regulated metabolites. To study the effect of belinostat on redox signaling Are-NRF2 pathway, luciferase reporter activity assay was done in stably transfected HepG2-C8 cells (containing pARE-TI-luciferase construct), followed by qPCR analysis of NRF2 and its target gene in NSCLC cells. <b>Key findings:</b> Metabolomic study revealed significantly altered metabolic levels related to redox homeostasis including tricarboxylic acid (TCA) cycle metabolites (citrate, aconitate, fumarate, malate and α-ketoglutarate); urea cycle metabolites (Arginine, ornithine, argino-succinate, aspartate and fumarate); and glutathione metabolism pathway (GSH/GSSG and NAD/NADH ratio) after belinostat treatment. ¹³C stable isotope labeling data indicates potential role of belinostat in creatine biosynthesis via methylation of guanidinoacetate. Moreover, belinostat inhibited NRF2 activity and downregulated the expression of NRF2 and its target gene NAD(P)H:quinone oxidoreductase 1 (NQO1). <b>Significance:</b> Belinostat is effective in killing KRAS-mutant lung cancer cells by regulating mitochondrial metabolism. <b>Keywords:</b> Belinostat; HDAC inhibitor; Mitochondrial metabolism; KRAS mutation; NRF2; Lung cancer This work was supported in part by institutional funds and and P30 ES005022 from the National Institute of Environmental Health Sciences (NIEHS)." @default.
• W4377029079 created "2023-05-19" @default.
• W4377029079 creator A5002961841 @default.
• W4377029079 creator A5006547884 @default.
• W4377029079 creator A5011053027 @default.
• W4377029079 creator A5049325403 @default.
• W4377029079 creator A5062381601 @default.
• W4377029079 date "2023-05-18" @default.
• W4377029079 modified "2023-09-29" @default.
• W4377029079 title "Histone Deacetylase Inhibitor Belinostat Regulates Metabolic Reprogramming in Killing Kras-Mutant Human Lung Cancer Cells" @default.
• W4377029079 doi "https://doi.org/10.1124/jpet.122.180950" @default.
• W4377029079 hasPublicationYear "2023" @default.
• W4377029079 type Work @default.
• W4377029079 citedByCount "0" @default.
• W4377029079 crossrefType "proceedings-article" @default.
• W4377029079 hasAuthorship W4377029079A5002961841 @default.
• W4377029079 hasAuthorship W4377029079A5006547884 @default.
• W4377029079 hasAuthorship W4377029079A5011053027 @default.
• W4377029079 hasAuthorship W4377029079A5049325403 @default.
• W4377029079 hasAuthorship W4377029079A5062381601 @default.
• W4377029079 hasBestOaLocation W43770290791 @default.
• W4377029079 hasConcept C104317684 @default.
• W4377029079 hasConcept C165135838 @default.
• W4377029079 hasConcept C185592680 @default.
• W4377029079 hasConcept C2776202225 @default.
• W4377029079 hasConcept C2778305200 @default.
• W4377029079 hasConcept C2781187634 @default.
• W4377029079 hasConcept C501734568 @default.
• W4377029079 hasConcept C502942594 @default.
• W4377029079 hasConcept C55493867 @default.
• W4377029079 hasConcept C62231903 @default.
• W4377029079 hasConcept C64927066 @default.
• W4377029079 hasConcept C86803240 @default.
• W4377029079 hasConceptScore W4377029079C104317684 @default.
• W4377029079 hasConceptScore W4377029079C165135838 @default.
• W4377029079 hasConceptScore W4377029079C185592680 @default.
• W4377029079 hasConceptScore W4377029079C2776202225 @default.
• W4377029079 hasConceptScore W4377029079C2778305200 @default.
• W4377029079 hasConceptScore W4377029079C2781187634 @default.
• W4377029079 hasConceptScore W4377029079C501734568 @default.
• W4377029079 hasConceptScore W4377029079C502942594 @default.
• W4377029079 hasConceptScore W4377029079C55493867 @default.
• W4377029079 hasConceptScore W4377029079C62231903 @default.
• W4377029079 hasConceptScore W4377029079C64927066 @default.
• W4377029079 hasConceptScore W4377029079C86803240 @default.
• W4377029079 hasLocation W43770290791 @default.
• W4377029079 hasOpenAccess W4377029079 @default.
• W4377029079 hasPrimaryLocation W43770290791 @default.
• W4377029079 hasRelatedWork W1964898256 @default.
• W4377029079 hasRelatedWork W2002457801 @default.
• W4377029079 hasRelatedWork W2089179463 @default.
• W4377029079 hasRelatedWork W2090929360 @default.
• W4377029079 hasRelatedWork W2167510303 @default.
• W4377029079 hasRelatedWork W2740863466 @default.
• W4377029079 hasRelatedWork W2767774564 @default.
• W4377029079 hasRelatedWork W2810278452 @default.
• W4377029079 hasRelatedWork W3164016378 @default.
• W4377029079 hasRelatedWork W4381337323 @default.
• W4377029079 isParatext "false" @default.
• W4377029079 isRetracted "false" @default.
• W4377029079 workType "article" @default.