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• W4377029287 abstract "<b>Abstract ID 52257</b> <b>Poster Board 96</b> Hfq is an RNA chaperone found in many bacteria that plays an important role in regulating gene expression. Hfq associates with small RNA molecules to form ribonucleoprotein (RNP) complexes, which can target and silence specific mRNAs by altering their stability or translation. Due to its important role in the cell, it is important that we understand the regulation of <i>hfq</i> expression at multiple levels. In this work, we are investigating the regulation of <i>hfq</i> expression by RNA modifications. RNA modifications are chemical changes to RNA molecules that ensure accuracy of the translation of the genetic code. Theses chemical modifications promote tight codon-anticodon interactions and stabilize the tRNA molecule, decreasing the probability of so that it is translation errors. We constructed an <i>hfq</i>-<i>lacZ</i> translation fusion in <i>Escherichia coli</i> K12 MG1655 and executed a targeted genetic screen for RNA modification mutants that may affect <i>hfq</i> expression. We compared the activity of wild type and mutant (in RNA modification genes) <i>hfq-lacZ</i> translational fusion in the absence of these RNA modification mutants. We measured <i>hfq</i>-<i>lacZ</i> activity in RNA modification mutants using high-throughput kinetic β-galactosidase assays. Finally, we measured Hfq protein levels in wild type and RNA modification mutant backgrounds using Western blot analysis. Our study shows the tRNA uridine 5-carboxymethylaminomethyl synthase, encoded by the <i>mnmG</i> gene, is necessary for full expression of <i>hfq</i> in <i>E. coli.</i> These RNA modification enzymes are highly conserved in bacterial and humans. Further, the lack of many RNA modifications have been associated with neurological diseases, cancer, and antibiotic resistance possibly due to defective gene expression." @default.
• W4377029287 created "2023-05-19" @default.
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• W4377029287 date "2023-05-18" @default.
• W4377029287 modified "2023-09-29" @default.
• W4377029287 title "Identification of Transfer RNA Modification Enzymes That Regulate a Bacterial RNA Chaperone Known as Hfq" @default.
• W4377029287 doi "https://doi.org/10.1124/jpet.122.522570" @default.
• W4377029287 hasPublicationYear "2023" @default.
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