FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4377029408> ?p ?o ?g. }

• W4377029408 abstract "<b>Abstract ID 16855</b> <b>Poster Board 372</b> Transient Receptor Potential Ankyrin 1 (TRPA1) and Vanilloid 1 (TRPV1) are nociceptive ion channels involving in pain sensation and development of neurogenic inflammation. These non-selective channels are located in the lipid rafts, the cholesterol-rich membrane domains of the plasma membrane of primary sensory neurons and peripheral nerve terminals. Cyclodextrins (CDs) are able to form inclusion complexes with cholesterol and deplete it from lipid rafts. We have already described that lipid raft disruption by Methyl-β-cyclodextrin (MCD) inhibited TRP ion channel function and has analgesic effect in animal models. We tested five different CD derivatives (Randomly methylated β-cyclodextrin: RAMEB, (2-Hydroxypropyl)-γ-cyclodextrin: HPGCD, (2-Hydroxyproyil)-β-cyclodextrin: HPBCD, Sulfobutylated β-cyclodextrin sodium salt: SBECD and (2-Hydroxy-3-N,N,N-trimethylamino) propyl-β cyclodextrin: QABCD; CycloLab Ltd.) in respect of their cytotoxicity (1, 3, 10, 50, 100 mM; 24 h) on chinese hamster ovary (CHO) cells with CellTiter-Glo® Luminescent Cell Viability Assay. MitoTracker™ Red CMXRos fluorescent dye was used to reveal the effect of 24-hour CD treatment on mitochondrial functioning of CHO cells. We performed radioactive ⁴⁵Ca²⁺-uptake measurements on TRPA1 and TRPV1 receptor-expressing CHO cells to detect alterations in receptor activation after CD treatment. In CellTiter-Glo® Luminescent Cell Viability Assay the methylated derivative RAMEB showed significant cytotoxic effect in 3.5 mM concentration, but none of the non-methylated derivatives decreased cell viability in 10 mM concentration. HPBCD treatment (1 mM and 10 mM) and QABCD treatment (10 mM) resulted in significantly increased fluorescence intensities of CHO cells’ mitochondria labeled with MitoTracker™ Red CMXRos. All of the investigated CDs were able to inhibit the ⁴⁵Ca²⁺-uptake in TRPA1 and TRPV1 receptor-expressing CHO cells in a concentration dependent manner. In conclusion, non-methylated derivatives have much lower cytotoxicity compared to RAMEB. HPBCD and QABCD affected significantly the mitochondrial function and all investigated CD derivatives were able to inhibit TRPA1 and TRPV1 channel activation, presumably via the disruption of lipid rafts. Targeting hydrophobic interactions of the protein-lipid interface might be promising as a novel mechanism of action in analgesia. <b>Support/Funding Information:</b> TKP2021-EGA-16, TKP2021-EGA-13, NKFIH-138936, RRF-2.3.1-21-2022-00015, KTIA_NAP_20017-1.2.1-NKP-2017-00002, ÚNKP-21-3-II New National Excellence Program of the Ministry for Innovation and Technology, Gedeon Richter Talentum Foundation" @default.
• W4377029408 created "2023-05-19" @default.
• W4377029408 creator A5005562973 @default.
• W4377029408 creator A5023403584 @default.
• W4377029408 creator A5025909021 @default.
• W4377029408 creator A5037712558 @default.
• W4377029408 creator A5042476250 @default.
• W4377029408 creator A5056590258 @default.
• W4377029408 creator A5091974015 @default.
• W4377029408 date "2023-05-18" @default.
• W4377029408 modified "2023-10-17" @default.
• W4377029408 title "Cyclodextrins decrease TRPV1 and TRPA1 ion channel activation via lipid raft disruption" @default.
• W4377029408 doi "https://doi.org/10.1124/jpet.122.168550" @default.
• W4377029408 hasPublicationYear "2023" @default.
• W4377029408 type Work @default.
• W4377029408 citedByCount "0" @default.
• W4377029408 crossrefType "proceedings-article" @default.
• W4377029408 hasAuthorship W4377029408A5005562973 @default.
• W4377029408 hasAuthorship W4377029408A5023403584 @default.
• W4377029408 hasAuthorship W4377029408A5025909021 @default.
• W4377029408 hasAuthorship W4377029408A5037712558 @default.
• W4377029408 hasAuthorship W4377029408A5042476250 @default.
• W4377029408 hasAuthorship W4377029408A5056590258 @default.
• W4377029408 hasAuthorship W4377029408A5091974015 @default.
• W4377029408 hasBestOaLocation W43770294081 @default.
• W4377029408 hasConcept C109316439 @default.
• W4377029408 hasConcept C12554922 @default.
• W4377029408 hasConcept C1491633281 @default.
• W4377029408 hasConcept C153911025 @default.
• W4377029408 hasConcept C155164980 @default.
• W4377029408 hasConcept C160145004 @default.
• W4377029408 hasConcept C170493617 @default.
• W4377029408 hasConcept C175656101 @default.
• W4377029408 hasConcept C185592680 @default.
• W4377029408 hasConcept C202751555 @default.
• W4377029408 hasConcept C2779433975 @default.
• W4377029408 hasConcept C2910962261 @default.
• W4377029408 hasConcept C53227056 @default.
• W4377029408 hasConcept C55493867 @default.
• W4377029408 hasConcept C79266657 @default.
• W4377029408 hasConcept C86803240 @default.
• W4377029408 hasConceptScore W4377029408C109316439 @default.
• W4377029408 hasConceptScore W4377029408C12554922 @default.
• W4377029408 hasConceptScore W4377029408C1491633281 @default.
• W4377029408 hasConceptScore W4377029408C153911025 @default.
• W4377029408 hasConceptScore W4377029408C155164980 @default.
• W4377029408 hasConceptScore W4377029408C160145004 @default.
• W4377029408 hasConceptScore W4377029408C170493617 @default.
• W4377029408 hasConceptScore W4377029408C175656101 @default.
• W4377029408 hasConceptScore W4377029408C185592680 @default.
• W4377029408 hasConceptScore W4377029408C202751555 @default.
• W4377029408 hasConceptScore W4377029408C2779433975 @default.
• W4377029408 hasConceptScore W4377029408C2910962261 @default.
• W4377029408 hasConceptScore W4377029408C53227056 @default.
• W4377029408 hasConceptScore W4377029408C55493867 @default.
• W4377029408 hasConceptScore W4377029408C79266657 @default.
• W4377029408 hasConceptScore W4377029408C86803240 @default.
• W4377029408 hasLocation W43770294081 @default.
• W4377029408 hasOpenAccess W4377029408 @default.
• W4377029408 hasPrimaryLocation W43770294081 @default.
• W4377029408 hasRelatedWork W2037651260 @default.
• W4377029408 hasRelatedWork W2041202471 @default.
• W4377029408 hasRelatedWork W2077959639 @default.
• W4377029408 hasRelatedWork W2083444342 @default.
• W4377029408 hasRelatedWork W2094955398 @default.
• W4377029408 hasRelatedWork W217881392 @default.
• W4377029408 hasRelatedWork W3032079354 @default.
• W4377029408 hasRelatedWork W3125378993 @default.
• W4377029408 hasRelatedWork W4376224025 @default.
• W4377029408 hasRelatedWork W4377029408 @default.
• W4377029408 isParatext "false" @default.
• W4377029408 isRetracted "false" @default.
• W4377029408 workType "article" @default.