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• W4377030698 endingPage "161" @default.
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• W4377030698 abstract "Quantum dots (QDs) containing zinc (Zn) and tellurium (Te) have low toxicity and excellent optoelectronic properties, which make them ideal fluorescent probes for use in environmental monitoring. However, their size/shape distribution synthesized by existing methods is not as good as that of other nanoparticles, thus limiting their application. Exploring whether this kind of QD can be biosynthesized and whether it can act as a nanoprobe are favorable attempts to expand the synthesis method and the application of QDs. Telluride QDs were biosynthesized in Escherichia coli cells. The nanoparticles were characterized by transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), energy dispersive X-ray spectroscopy (EDX), and inductively coupled plasma-atomic emission spectrometry (ICP‒AES), indicating that they were Zn3STe2 QDs. The QDs were monodispersed, spherical and fluorescently stable, with a uniform particle size of 3.05 ± 0.48 nm. The biosynthesis conditions of the QDs, including substrate concentrations and their process time, were optimized respectively. It was verified that the cysE and cysK genes were involved in the biosynthesis of telluride QDs. The biosynthesis ability of the QDs was improved by knocking out the tehB gene and overexpressing the pckA gene. Escherichia coli BW25113 cells that synthesized Zn3STe2 QDs were used as environmentally friendly fluorescent bioprobes to specifically select and quantitatively detect Fe3+ in water with a low limit of detection (2.62 μM). The fluorescent cells were also photobleach resistant and had good fluorescence stability. This study expands on the synthesis method of telluride QDs and the application of fluorescent probes." @default.
• W4377030698 created "2023-05-19" @default.
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• W4377030698 date "2023-07-01" @default.
• W4377030698 modified "2023-10-15" @default.
• W4377030698 title "Biosynthesis of uniform fluorescent-stable telluride quantum dots in Escherichia coli and its detection of Fe3+ in water" @default.
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• W4377030698 doi "https://doi.org/10.1016/j.bbrc.2023.05.060" @default.
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