Matches in SemOpenAlex for { <https://semopenalex.org/work/W4377041815> ?p ?o ?g. }
- W4377041815 abstract "Unexplained recurrent spontaneous abortion (URSA) is a severe challenge to reproductive females worldwide, and its etiology and pathogenesis have not yet been fully clarified. Abnormal intercellular communication between macrophages (Mφ) and decidual stromal cells (DSCs) or trophoblasts has been supposed to be the key to URSA. However, the exact molecular mechanisms in the crosstalk are not yet well understood. This study aimed to explore the potential molecule mechanism that may be involved in the communication between Mφ and DSC or trophoblast cells and determine their diagnostic characteristics by using the integrated research strategy of bioinformatics analysis, machine learning and experiments. First, microarrays of decidual tissue (GSE26787, GSE165004) and placenta tissue (GSE22490) in patients with URSA, as well as microarrays involving induced decidualization (GSE94644) and macrophage polarization in vitro (GSE30595) were derived from the gene expression omnibus (GEO) database. And 721 decidua-differentially expressed genes (DEGs), 613 placenta-DEGs, 510 Mφ polarization DEGs were obtained in URSA by differential expression analysis. Then, the protein-protein interaction (PPI) network was constructed, and the hub genes were identified by CytoHubba in Cytoscape software and validated by real-time PCR assay. Subsequently, immune enrichment analysis on decidua-DEGs and placenta-DEGs by ClueGO verified their regulation effects on Mφ. Besides, functional enrichment analysis was performed on Mφ polarization DEGs and the essential module genes derived from the weighted gene co-expression network analysis (WGCNA) to uncover the biological function that were related to abnormal polarization of Mφ. Furthermore, we screened out 29, 43 and 22 secreted protein-encoding genes from DSC-DEGs, placenta-DEGs and Mφ polarization DEGs, respectively. Besides, the hub secreted-protein-encoding genes were screened by CytoHubba. Moreover, we conducted functional enrichment analysis on these genes. And spearman correlation analysis between hub secreted-protein-encoding genes from donor cells and hub genes in recipient cells was performed to further understand the molecular mechanism of intercellular communication further. Moreover, signature genes with diagnostic value were screened from secreted protein-encoding genes by machine learning and validated by immunofluorescence co-localization analysis with clinical samples. Finally, three biomarkers of DSCs (FGF9, IL1R2, NID2) and three biomarkers of Mφ (CFB, NID2, CXCL11) were obtained. In conclusion, this project provides new ideas for understanding the mechanism regulatory network of intercellular communication involving macrophages at the maternal-fetal interface of URSA. Also, it provides innovative insights for the diagnosis and treatment of URSA." @default.
- W4377041815 created "2023-05-19" @default.
- W4377041815 creator A5017559970 @default.
- W4377041815 creator A5027432922 @default.
- W4377041815 creator A5029026931 @default.
- W4377041815 creator A5045901985 @default.
- W4377041815 creator A5069057903 @default.
- W4377041815 date "2023-05-17" @default.
- W4377041815 modified "2023-09-26" @default.
- W4377041815 title "Intercellular communication involving macrophages at the maternal-fetal interface may be a pivotal mechanism of URSA: a novel discovery from transcriptomic data" @default.
- W4377041815 cites W1852269776 @default.
- W4377041815 cites W2036106861 @default.
- W4377041815 cites W2049014099 @default.
- W4377041815 cites W2050104744 @default.
- W4377041815 cites W2067786443 @default.
- W4377041815 cites W2076975743 @default.
- W4377041815 cites W2083060219 @default.
- W4377041815 cites W2097360283 @default.
- W4377041815 cites W2103912979 @default.
- W4377041815 cites W2118258530 @default.
- W4377041815 cites W2119975890 @default.
- W4377041815 cites W2131105131 @default.
- W4377041815 cites W2141894278 @default.
- W4377041815 cites W2159675211 @default.
- W4377041815 cites W2164487100 @default.
- W4377041815 cites W2169589032 @default.
- W4377041815 cites W2169741037 @default.
- W4377041815 cites W2175541615 @default.
- W4377041815 cites W243303449 @default.
- W4377041815 cites W2537623931 @default.
- W4377041815 cites W2586115964 @default.
- W4377041815 cites W2603446204 @default.
- W4377041815 cites W2762148394 @default.
- W4377041815 cites W2765467770 @default.
- W4377041815 cites W2789351798 @default.
- W4377041815 cites W2793585023 @default.
- W4377041815 cites W2796224907 @default.
- W4377041815 cites W2809000676 @default.
- W4377041815 cites W2889588124 @default.
- W4377041815 cites W2902575310 @default.
- W4377041815 cites W2946173685 @default.
- W4377041815 cites W2958281396 @default.
- W4377041815 cites W3006038773 @default.
- W4377041815 cites W3094772815 @default.
- W4377041815 cites W3106887675 @default.
- W4377041815 cites W3111446071 @default.
- W4377041815 cites W3119494171 @default.
- W4377041815 cites W3124559198 @default.
- W4377041815 cites W3130165291 @default.
- W4377041815 cites W3138251459 @default.
- W4377041815 cites W3158124734 @default.
- W4377041815 cites W3162035493 @default.
- W4377041815 cites W3172312035 @default.
- W4377041815 cites W3183689528 @default.
- W4377041815 cites W3199017926 @default.
- W4377041815 cites W3207690207 @default.
- W4377041815 cites W3208235554 @default.
- W4377041815 cites W3210515059 @default.
- W4377041815 cites W3214157627 @default.
- W4377041815 cites W3215227144 @default.
- W4377041815 cites W4200133820 @default.
- W4377041815 cites W4207000890 @default.
- W4377041815 cites W4214863055 @default.
- W4377041815 cites W4214905002 @default.
- W4377041815 cites W4220752907 @default.
- W4377041815 cites W4223891923 @default.
- W4377041815 cites W4224225914 @default.
- W4377041815 cites W4224952138 @default.
- W4377041815 cites W4238278966 @default.
- W4377041815 cites W4280593383 @default.
- W4377041815 cites W4281399944 @default.
- W4377041815 cites W4283739367 @default.
- W4377041815 cites W4293767581 @default.
- W4377041815 cites W4294541781 @default.
- W4377041815 cites W4307044651 @default.
- W4377041815 cites W4310705349 @default.
- W4377041815 cites W4310862531 @default.
- W4377041815 cites W3182262489 @default.
- W4377041815 doi "https://doi.org/10.3389/fendo.2023.973930" @default.
- W4377041815 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37265689" @default.
- W4377041815 hasPublicationYear "2023" @default.
- W4377041815 type Work @default.
- W4377041815 citedByCount "1" @default.
- W4377041815 countsByYear W43770418152023 @default.
- W4377041815 crossrefType "journal-article" @default.
- W4377041815 hasAuthorship W4377041815A5017559970 @default.
- W4377041815 hasAuthorship W4377041815A5027432922 @default.
- W4377041815 hasAuthorship W4377041815A5029026931 @default.
- W4377041815 hasAuthorship W4377041815A5045901985 @default.
- W4377041815 hasAuthorship W4377041815A5069057903 @default.
- W4377041815 hasBestOaLocation W43770418151 @default.
- W4377041815 hasConcept C104317684 @default.
- W4377041815 hasConcept C127716648 @default.
- W4377041815 hasConcept C150194340 @default.
- W4377041815 hasConcept C162317418 @default.
- W4377041815 hasConcept C16930146 @default.
- W4377041815 hasConcept C172680121 @default.
- W4377041815 hasConcept C18431079 @default.
- W4377041815 hasConcept C186836561 @default.
- W4377041815 hasConcept C2776682551 @default.