Matches in SemOpenAlex for { <https://semopenalex.org/work/W4377088904> ?p ?o ?g. }
- W4377088904 endingPage "124808" @default.
- W4377088904 startingPage "124808" @default.
- W4377088904 abstract "Raffinose family oligosaccharides (RFOs) in food are the main factors causing flatulence in Irritable Bowel Syndrome (IBS) patients and the development of effective approaches for reducing food-derived RFOs is of paramount importance. In this study, polyvinyl alcohol (PVA)-chitosan (CS)-glycidyl methacrylate (GMA) immobilized α-galactosidase was prepared by the directional freezing-assisted salting-out technique, aimed to hydrolyze RFOs. SEM, FTIR, XPS, fluorescence and UV characterization results demonstrated that α-galactosidase was successfully cross-linked in the PVA-CS-GMA hydrogels, forming a distinct porous stable network through the covalent bond between the enzyme and the carrier. Mechanical performance and swelling capacity analysis illustrated that α-gal @ PVA-CS-GMA not only had suitable strength and toughness for longer durability, but also exhibited high water content and swelling capacity for better retention of catalytic activity. The enzymatic properties of α-gal @ PVA-CS-GMA showed an improved Km value, pH and temperature tolerance range, anti-enzymatic inhibitor (melibiose) activity compared to the free α-galactosidase and its reusability was at least 12 times with prolonged storage stability. Finally, it was successfully applied in the hydrolysis of RFOs in soybeans. These findings provide a new strategy for the development of α-galactosidase immobilization system to biological transform the RFOs components in the food for diet intervention of IBS." @default.
- W4377088904 created "2023-05-20" @default.
- W4377088904 creator A5015096605 @default.
- W4377088904 creator A5023986556 @default.
- W4377088904 creator A5026160699 @default.
- W4377088904 creator A5029175932 @default.
- W4377088904 creator A5049816813 @default.
- W4377088904 creator A5081585246 @default.
- W4377088904 creator A5081778907 @default.
- W4377088904 date "2023-07-01" @default.
- W4377088904 modified "2023-09-29" @default.
- W4377088904 title "Immobilization of α-galactosidase in polyvinyl alcohol-chitosan-glycidyl methacrylate hydrogels based on directional freezing-assisted salting-out strategy for hydrolysis of RFOs" @default.
- W4377088904 cites W1964813916 @default.
- W4377088904 cites W1992634272 @default.
- W4377088904 cites W2005548003 @default.
- W4377088904 cites W2008925142 @default.
- W4377088904 cites W2044982609 @default.
- W4377088904 cites W2056711702 @default.
- W4377088904 cites W2097014718 @default.
- W4377088904 cites W2137122407 @default.
- W4377088904 cites W2149612297 @default.
- W4377088904 cites W2176713976 @default.
- W4377088904 cites W2327894252 @default.
- W4377088904 cites W2529309698 @default.
- W4377088904 cites W2530293980 @default.
- W4377088904 cites W2553830786 @default.
- W4377088904 cites W2571384434 @default.
- W4377088904 cites W2577225940 @default.
- W4377088904 cites W2605678043 @default.
- W4377088904 cites W2774662697 @default.
- W4377088904 cites W2783336634 @default.
- W4377088904 cites W2791380587 @default.
- W4377088904 cites W2791426131 @default.
- W4377088904 cites W2807540958 @default.
- W4377088904 cites W2890329489 @default.
- W4377088904 cites W2908622111 @default.
- W4377088904 cites W2910843994 @default.
- W4377088904 cites W2931622581 @default.
- W4377088904 cites W2967023035 @default.
- W4377088904 cites W2967965298 @default.
- W4377088904 cites W2985460649 @default.
- W4377088904 cites W3015678014 @default.
- W4377088904 cites W3016802365 @default.
- W4377088904 cites W3020340386 @default.
- W4377088904 cites W3022335475 @default.
- W4377088904 cites W3026312221 @default.
- W4377088904 cites W3028843770 @default.
- W4377088904 cites W3047106352 @default.
- W4377088904 cites W3087639210 @default.
- W4377088904 cites W3097355792 @default.
- W4377088904 cites W3127344350 @default.
- W4377088904 cites W3129596738 @default.
- W4377088904 cites W3134261458 @default.
- W4377088904 cites W3169894911 @default.
- W4377088904 cites W3195856598 @default.
- W4377088904 cites W3201338143 @default.
- W4377088904 cites W3205819835 @default.
- W4377088904 cites W4200029733 @default.
- W4377088904 cites W4210672541 @default.
- W4377088904 cites W4220842864 @default.
- W4377088904 cites W4221037433 @default.
- W4377088904 cites W4224942348 @default.
- W4377088904 cites W4224995837 @default.
- W4377088904 cites W4281734117 @default.
- W4377088904 cites W4281743620 @default.
- W4377088904 cites W4289260378 @default.
- W4377088904 cites W4293812218 @default.
- W4377088904 cites W4324367553 @default.
- W4377088904 doi "https://doi.org/10.1016/j.ijbiomac.2023.124808" @default.
- W4377088904 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37211074" @default.
- W4377088904 hasPublicationYear "2023" @default.
- W4377088904 type Work @default.
- W4377088904 citedByCount "0" @default.
- W4377088904 crossrefType "journal-article" @default.
- W4377088904 hasAuthorship W4377088904A5015096605 @default.
- W4377088904 hasAuthorship W4377088904A5023986556 @default.
- W4377088904 hasAuthorship W4377088904A5026160699 @default.
- W4377088904 hasAuthorship W4377088904A5029175932 @default.
- W4377088904 hasAuthorship W4377088904A5049816813 @default.
- W4377088904 hasAuthorship W4377088904A5081585246 @default.
- W4377088904 hasAuthorship W4377088904A5081778907 @default.
- W4377088904 hasConcept C108586683 @default.
- W4377088904 hasConcept C127413603 @default.
- W4377088904 hasConcept C160892712 @default.
- W4377088904 hasConcept C178790620 @default.
- W4377088904 hasConcept C185592680 @default.
- W4377088904 hasConcept C188027245 @default.
- W4377088904 hasConcept C2778540859 @default.
- W4377088904 hasConcept C2778551929 @default.
- W4377088904 hasConcept C2778597767 @default.
- W4377088904 hasConcept C2779732960 @default.
- W4377088904 hasConcept C2779998883 @default.
- W4377088904 hasConcept C2780801859 @default.
- W4377088904 hasConcept C42360764 @default.
- W4377088904 hasConcept C44228677 @default.
- W4377088904 hasConcept C521977710 @default.
- W4377088904 hasConcept C94412978 @default.
- W4377088904 hasConceptScore W4377088904C108586683 @default.