Matches in SemOpenAlex for { <https://semopenalex.org/work/W4377103624> ?p ?o ?g. }
- W4377103624 endingPage "1192" @default.
- W4377103624 startingPage "1192" @default.
- W4377103624 abstract "Human adenovirus 55 (HAdV-55) has recently caused outbreaks of acute respiratory disease (ARD), posing a significant public threat to civilians and military trainees. Efforts to develop antiviral inhibitors and quantify neutralizing antibodies require an experimental system to rapidly monitor viral infections, which can be achieved through the use of a plasmid that can produce an infectious virus. Here, we used a bacteria-mediated recombination approach to construct a full-length infectious cDNA clone, pAd55-FL, containing the whole genome of HadV-55. Then, the green fluorescent protein expression cassette was assembled into pAd55-FL to replace the E3 region to obtain a recombinant plasmid of pAd55-dE3-EGFP. The rescued recombinant virus rAdv55-dE3-EGFP is genetically stable and replicates similarly to the wild-type virus in cell culture. The virus rAdv55-dE3-EGFP can be used to quantify neutralizing antibody activity in sera samples, producing results in concordance with the cytopathic effect (CPE)-based microneutralization assay. Using an rAdv55-dE3-EGFP infection of A549 cells, we showed that the assay could be used for antiviral screening. Our findings suggest that the rAdv55-dE3-EGFP-based high-throughput assay provides a reliable tool for rapid neutralization testing and antiviral screening for HAdV-55." @default.
- W4377103624 created "2023-05-20" @default.
- W4377103624 creator A5006223465 @default.
- W4377103624 creator A5006887310 @default.
- W4377103624 creator A5016068311 @default.
- W4377103624 creator A5021267794 @default.
- W4377103624 creator A5032145382 @default.
- W4377103624 creator A5050311492 @default.
- W4377103624 creator A5051301839 @default.
- W4377103624 creator A5066066351 @default.
- W4377103624 creator A5068001596 @default.
- W4377103624 creator A5070652968 @default.
- W4377103624 creator A5074903674 @default.
- W4377103624 creator A5075206100 @default.
- W4377103624 date "2023-05-18" @default.
- W4377103624 modified "2023-09-26" @default.
- W4377103624 title "Generation and Characterization of a Replication-Competent Human Adenovirus Type 55 Encoding EGFP" @default.
- W4377103624 cites W1490269903 @default.
- W4377103624 cites W1998385063 @default.
- W4377103624 cites W2002760980 @default.
- W4377103624 cites W2004736524 @default.
- W4377103624 cites W2014774026 @default.
- W4377103624 cites W2032052837 @default.
- W4377103624 cites W2036869557 @default.
- W4377103624 cites W2046730970 @default.
- W4377103624 cites W2051164613 @default.
- W4377103624 cites W2051568749 @default.
- W4377103624 cites W2063825413 @default.
- W4377103624 cites W2093280481 @default.
- W4377103624 cites W2093359246 @default.
- W4377103624 cites W2123883267 @default.
- W4377103624 cites W2328476123 @default.
- W4377103624 cites W2472344936 @default.
- W4377103624 cites W2487775023 @default.
- W4377103624 cites W2508490597 @default.
- W4377103624 cites W2623865818 @default.
- W4377103624 cites W2761517526 @default.
- W4377103624 cites W2804713263 @default.
- W4377103624 cites W2902102773 @default.
- W4377103624 cites W2903680570 @default.
- W4377103624 cites W2910346224 @default.
- W4377103624 cites W2911899687 @default.
- W4377103624 cites W2913669888 @default.
- W4377103624 cites W2947470486 @default.
- W4377103624 cites W2986345671 @default.
- W4377103624 cites W3081902156 @default.
- W4377103624 cites W3091676062 @default.
- W4377103624 cites W3095121897 @default.
- W4377103624 cites W3189758897 @default.
- W4377103624 cites W3203233023 @default.
- W4377103624 cites W3217153718 @default.
- W4377103624 cites W4200084141 @default.
- W4377103624 cites W4221076659 @default.
- W4377103624 cites W4283015561 @default.
- W4377103624 cites W4283080634 @default.
- W4377103624 cites W4293683088 @default.
- W4377103624 cites W4310801672 @default.
- W4377103624 cites W4318218727 @default.
- W4377103624 cites W4319322032 @default.
- W4377103624 cites W4321481351 @default.
- W4377103624 doi "https://doi.org/10.3390/v15051192" @default.
- W4377103624 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37243276" @default.
- W4377103624 hasPublicationYear "2023" @default.
- W4377103624 type Work @default.
- W4377103624 citedByCount "0" @default.
- W4377103624 crossrefType "journal-article" @default.
- W4377103624 hasAuthorship W4377103624A5006223465 @default.
- W4377103624 hasAuthorship W4377103624A5006887310 @default.
- W4377103624 hasAuthorship W4377103624A5016068311 @default.
- W4377103624 hasAuthorship W4377103624A5021267794 @default.
- W4377103624 hasAuthorship W4377103624A5032145382 @default.
- W4377103624 hasAuthorship W4377103624A5050311492 @default.
- W4377103624 hasAuthorship W4377103624A5051301839 @default.
- W4377103624 hasAuthorship W4377103624A5066066351 @default.
- W4377103624 hasAuthorship W4377103624A5068001596 @default.
- W4377103624 hasAuthorship W4377103624A5070652968 @default.
- W4377103624 hasAuthorship W4377103624A5074903674 @default.
- W4377103624 hasAuthorship W4377103624A5075206100 @default.
- W4377103624 hasBestOaLocation W43771036241 @default.
- W4377103624 hasConcept C104317684 @default.
- W4377103624 hasConcept C142613039 @default.
- W4377103624 hasConcept C153911025 @default.
- W4377103624 hasConcept C159047783 @default.
- W4377103624 hasConcept C159654299 @default.
- W4377103624 hasConcept C22744801 @default.
- W4377103624 hasConcept C2522874641 @default.
- W4377103624 hasConcept C2775996393 @default.
- W4377103624 hasConcept C2779261636 @default.
- W4377103624 hasConcept C39060996 @default.
- W4377103624 hasConcept C40767141 @default.
- W4377103624 hasConcept C54355233 @default.
- W4377103624 hasConcept C86803240 @default.
- W4377103624 hasConceptScore W4377103624C104317684 @default.
- W4377103624 hasConceptScore W4377103624C142613039 @default.
- W4377103624 hasConceptScore W4377103624C153911025 @default.
- W4377103624 hasConceptScore W4377103624C159047783 @default.
- W4377103624 hasConceptScore W4377103624C159654299 @default.
- W4377103624 hasConceptScore W4377103624C22744801 @default.