Matches in SemOpenAlex for { <https://semopenalex.org/work/W4377104328> ?p ?o ?g. }
- W4377104328 endingPage "1417" @default.
- W4377104328 startingPage "1417" @default.
- W4377104328 abstract "A newly developed therapy using effective-mononuclear cells (E-MNCs) is reportedly effective against radiation-damaged salivary glands (SGs) due to anti-inflammatory and revascularization effects. However, the cellular working mechanism of E-MNC therapy in SGs remains to be elucidated. In this study, E-MNCs were induced from peripheral blood mononuclear cells (PBMNCs) by culture for 5–7 days in medium supplemented with five specific recombinant proteins (5G-culture). We analyzed the anti-inflammatory characteristics of macrophage fraction of E-MNCs using a co-culture model with CD3/CD28-stimulated PBMNCs. To test therapeutic efficacy in vivo, either E-MNCs or E-MNCs depleted of CD11b-positive cells were transplanted intraglandularly into mice with radiation-damaged SGs. Following transplantation, SG function recovery and immunohistochemical analyses of harvested SGs were assessed to determine if CD11b-positive macrophages contributed to tissue regeneration. The results indicated that CD11b/CD206-positive (M2-like) macrophages were specifically induced in E-MNCs during 5G-culture, and Msr1- and galectin3-positive cells (immunomodulatory macrophages) were predominant. CD11b-positive fraction of E-MNCs significantly inhibited the expression of inflammation-related genes in CD3/CD28-stimulated PBMNCs. Transplanted E-MNCs exhibited a therapeutic effect on saliva secretion and reduced tissue fibrosis in radiation-damaged SGs, whereas E-MNCs depleted of CD11b-positive cells and radiated controls did not. Immunohistochemical analyses revealed HMGB1 phagocytosis and IGF1 secretion by CD11b/Msr1-positive macrophages from both transplanted E-MNCs and host M2-macrophages. Thus, the anti-inflammatory and tissue-regenerative effects observed in E-MNC therapy against radiation-damaged SGs can be partly explained by the immunomodulatory effect of M2-dominant macrophage fraction." @default.
- W4377104328 created "2023-05-20" @default.
- W4377104328 creator A5001764588 @default.
- W4377104328 creator A5039044606 @default.
- W4377104328 creator A5065165297 @default.
- W4377104328 creator A5068210817 @default.
- W4377104328 creator A5070784603 @default.
- W4377104328 creator A5071840669 @default.
- W4377104328 creator A5072523216 @default.
- W4377104328 creator A5072879091 @default.
- W4377104328 creator A5084017363 @default.
- W4377104328 creator A5087299256 @default.
- W4377104328 date "2023-05-17" @default.
- W4377104328 modified "2023-10-10" @default.
- W4377104328 title "Immunomodulatory Macrophages Enable E-MNC Therapy for Radiation-Induced Salivary Gland Hypofunction" @default.
- W4377104328 cites W1559873725 @default.
- W4377104328 cites W1972534236 @default.
- W4377104328 cites W1979173328 @default.
- W4377104328 cites W1995276725 @default.
- W4377104328 cites W2015375064 @default.
- W4377104328 cites W2017453832 @default.
- W4377104328 cites W2048228561 @default.
- W4377104328 cites W2048761824 @default.
- W4377104328 cites W2066718551 @default.
- W4377104328 cites W2072172632 @default.
- W4377104328 cites W2097937723 @default.
- W4377104328 cites W2105605694 @default.
- W4377104328 cites W2118797865 @default.
- W4377104328 cites W2124730679 @default.
- W4377104328 cites W2139515703 @default.
- W4377104328 cites W2153592558 @default.
- W4377104328 cites W2161434409 @default.
- W4377104328 cites W2165795181 @default.
- W4377104328 cites W2277014653 @default.
- W4377104328 cites W2462912221 @default.
- W4377104328 cites W2468452764 @default.
- W4377104328 cites W2519312034 @default.
- W4377104328 cites W2548291598 @default.
- W4377104328 cites W2575913984 @default.
- W4377104328 cites W2591115003 @default.
- W4377104328 cites W2606737383 @default.
- W4377104328 cites W2606914311 @default.
- W4377104328 cites W2782685575 @default.
- W4377104328 cites W2809930906 @default.
- W4377104328 cites W2810051543 @default.
- W4377104328 cites W2890623226 @default.
- W4377104328 cites W2900422864 @default.
- W4377104328 cites W2945823639 @default.
- W4377104328 cites W2969681245 @default.
- W4377104328 cites W2980780786 @default.
- W4377104328 cites W2982114022 @default.
- W4377104328 cites W2982293328 @default.
- W4377104328 cites W3037123153 @default.
- W4377104328 cites W3039764378 @default.
- W4377104328 cites W3082925174 @default.
- W4377104328 cites W3188459170 @default.
- W4377104328 cites W4224094228 @default.
- W4377104328 cites W4281870791 @default.
- W4377104328 cites W4284964174 @default.
- W4377104328 cites W4295540315 @default.
- W4377104328 doi "https://doi.org/10.3390/cells12101417" @default.
- W4377104328 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37408251" @default.
- W4377104328 hasPublicationYear "2023" @default.
- W4377104328 type Work @default.
- W4377104328 citedByCount "1" @default.
- W4377104328 countsByYear W43771043282023 @default.
- W4377104328 crossrefType "journal-article" @default.
- W4377104328 hasAuthorship W4377104328A5001764588 @default.
- W4377104328 hasAuthorship W4377104328A5039044606 @default.
- W4377104328 hasAuthorship W4377104328A5065165297 @default.
- W4377104328 hasAuthorship W4377104328A5068210817 @default.
- W4377104328 hasAuthorship W4377104328A5070784603 @default.
- W4377104328 hasAuthorship W4377104328A5071840669 @default.
- W4377104328 hasAuthorship W4377104328A5072523216 @default.
- W4377104328 hasAuthorship W4377104328A5072879091 @default.
- W4377104328 hasAuthorship W4377104328A5084017363 @default.
- W4377104328 hasAuthorship W4377104328A5087299256 @default.
- W4377104328 hasBestOaLocation W43771043281 @default.
- W4377104328 hasConcept C137061746 @default.
- W4377104328 hasConcept C142724271 @default.
- W4377104328 hasConcept C150903083 @default.
- W4377104328 hasConcept C160448771 @default.
- W4377104328 hasConcept C171779818 @default.
- W4377104328 hasConcept C185592680 @default.
- W4377104328 hasConcept C202751555 @default.
- W4377104328 hasConcept C203014093 @default.
- W4377104328 hasConcept C204232928 @default.
- W4377104328 hasConcept C207001950 @default.
- W4377104328 hasConcept C2776460901 @default.
- W4377104328 hasConcept C2776914184 @default.
- W4377104328 hasConcept C2779244956 @default.
- W4377104328 hasConcept C2780559512 @default.
- W4377104328 hasConcept C55493867 @default.
- W4377104328 hasConcept C71924100 @default.
- W4377104328 hasConcept C86803240 @default.
- W4377104328 hasConcept C8891405 @default.
- W4377104328 hasConceptScore W4377104328C137061746 @default.
- W4377104328 hasConceptScore W4377104328C142724271 @default.