Matches in SemOpenAlex for { <https://semopenalex.org/work/W4377141808> ?p ?o ?g. }
- W4377141808 endingPage "108985" @default.
- W4377141808 startingPage "108985" @default.
- W4377141808 abstract "Polyethylene terephthalate (PET) is a globally mismanaged plastic waste. Microbial degradation of plastics is a green and sustainable technology and very few studies report biocatalysts with plastic degradation potential. The present study aims to investigate the PETase from the evolution of IsPETase. Through NCBI database scanning, Aquabacterium parvum lipase (ApLip) was identified as an evolutionary intermediate between conventional lipases and IsPETase based on 16 S rRNA, amino acid sequences and protein structural similarity by simulation. The codon adaptation index (CAI) was used for ApLip to obtain ApLip-Ec and its protein expression was enhanced in Escherichia coli Lemo21(DE3) with a high catalytic efficiency of 11341 mM−1 min−1 based on p-nitrophenyl butyrate (pNPB) as a substrate. In vitro testing for PET degradation using 5.5 µM ApLip-Ec released 5.9 µM BHET after 48 h. Moreover, the synergism effect of ApLip-Ec and MHETase was explored. Successful PET degradation not only existed in Ideonella sakaiensis, but also evolved from structurally similar cutinases and lipases in nature." @default.
- W4377141808 created "2023-05-21" @default.
- W4377141808 creator A5003549962 @default.
- W4377141808 creator A5005472214 @default.
- W4377141808 creator A5007694164 @default.
- W4377141808 creator A5010348070 @default.
- W4377141808 creator A5032965110 @default.
- W4377141808 creator A5066766141 @default.
- W4377141808 creator A5081764779 @default.
- W4377141808 date "2023-08-01" @default.
- W4377141808 modified "2023-09-27" @default.
- W4377141808 title "Heterologous expression and characterization of Aquabacterium parvum lipase, a close relative of Ideonella sakaiensis PETase in Escherichia coli" @default.
- W4377141808 cites W1803102843 @default.
- W4377141808 cites W1894674957 @default.
- W4377141808 cites W1975993749 @default.
- W4377141808 cites W1988992477 @default.
- W4377141808 cites W1991263269 @default.
- W4377141808 cites W2007290227 @default.
- W4377141808 cites W2018590530 @default.
- W4377141808 cites W2025785727 @default.
- W4377141808 cites W2042706604 @default.
- W4377141808 cites W2073495854 @default.
- W4377141808 cites W2087173992 @default.
- W4377141808 cites W2100100451 @default.
- W4377141808 cites W2106510392 @default.
- W4377141808 cites W2107624359 @default.
- W4377141808 cites W2127505738 @default.
- W4377141808 cites W2140481794 @default.
- W4377141808 cites W2168612976 @default.
- W4377141808 cites W2259283565 @default.
- W4377141808 cites W2294707565 @default.
- W4377141808 cites W2409257097 @default.
- W4377141808 cites W2498068055 @default.
- W4377141808 cites W2774227215 @default.
- W4377141808 cites W2883813712 @default.
- W4377141808 cites W2889859816 @default.
- W4377141808 cites W2999690316 @default.
- W4377141808 cites W3082705272 @default.
- W4377141808 cites W3088201933 @default.
- W4377141808 cites W3100128741 @default.
- W4377141808 cites W3107998901 @default.
- W4377141808 cites W3156330672 @default.
- W4377141808 cites W3163742709 @default.
- W4377141808 cites W3181671191 @default.
- W4377141808 cites W3184012720 @default.
- W4377141808 cites W3189785920 @default.
- W4377141808 cites W3200683494 @default.
- W4377141808 cites W3209387586 @default.
- W4377141808 cites W4206500201 @default.
- W4377141808 cites W4210437765 @default.
- W4377141808 cites W4214674682 @default.
- W4377141808 cites W4223552703 @default.
- W4377141808 cites W4224988655 @default.
- W4377141808 cites W4306167586 @default.
- W4377141808 cites W4318055232 @default.
- W4377141808 doi "https://doi.org/10.1016/j.bej.2023.108985" @default.
- W4377141808 hasPublicationYear "2023" @default.
- W4377141808 type Work @default.
- W4377141808 citedByCount "0" @default.
- W4377141808 crossrefType "journal-article" @default.
- W4377141808 hasAuthorship W4377141808A5003549962 @default.
- W4377141808 hasAuthorship W4377141808A5005472214 @default.
- W4377141808 hasAuthorship W4377141808A5007694164 @default.
- W4377141808 hasAuthorship W4377141808A5010348070 @default.
- W4377141808 hasAuthorship W4377141808A5032965110 @default.
- W4377141808 hasAuthorship W4377141808A5066766141 @default.
- W4377141808 hasAuthorship W4377141808A5081764779 @default.
- W4377141808 hasConcept C104317684 @default.
- W4377141808 hasConcept C108305142 @default.
- W4377141808 hasConcept C181199279 @default.
- W4377141808 hasConcept C185592680 @default.
- W4377141808 hasConcept C18903297 @default.
- W4377141808 hasConcept C2777289219 @default.
- W4377141808 hasConcept C2779679103 @default.
- W4377141808 hasConcept C2779697368 @default.
- W4377141808 hasConcept C40767141 @default.
- W4377141808 hasConcept C41008148 @default.
- W4377141808 hasConcept C547475151 @default.
- W4377141808 hasConcept C55493867 @default.
- W4377141808 hasConcept C76155785 @default.
- W4377141808 hasConcept C86803240 @default.
- W4377141808 hasConceptScore W4377141808C104317684 @default.
- W4377141808 hasConceptScore W4377141808C108305142 @default.
- W4377141808 hasConceptScore W4377141808C181199279 @default.
- W4377141808 hasConceptScore W4377141808C185592680 @default.
- W4377141808 hasConceptScore W4377141808C18903297 @default.
- W4377141808 hasConceptScore W4377141808C2777289219 @default.
- W4377141808 hasConceptScore W4377141808C2779679103 @default.
- W4377141808 hasConceptScore W4377141808C2779697368 @default.
- W4377141808 hasConceptScore W4377141808C40767141 @default.
- W4377141808 hasConceptScore W4377141808C41008148 @default.
- W4377141808 hasConceptScore W4377141808C547475151 @default.
- W4377141808 hasConceptScore W4377141808C55493867 @default.
- W4377141808 hasConceptScore W4377141808C76155785 @default.
- W4377141808 hasConceptScore W4377141808C86803240 @default.
- W4377141808 hasFunder F4320322795 @default.
- W4377141808 hasLocation W43771418081 @default.
- W4377141808 hasOpenAccess W4377141808 @default.