Matches in SemOpenAlex for { <https://semopenalex.org/work/W4377194249> ?p ?o ?g. }
- W4377194249 abstract "Abstract Isocitrate lyase (ICL) isoform 2 is an essential enzyme for some clinical Mycobacterium tuberculosis ( Mtb ) strains during infection. In the laboratory Mtb strain H37Rv, the icl2 gene encodes two distinct gene products – Rv1915 and Rv1916 – due to a frameshift mutation. This study aims to characterise these two gene products to understand their structure and function. While we were unable to produce Rv1915 recombinantly, soluble Rv1916 was obtained with sufficient yield for characterisation. Kinetic studies using UV‐visible spectrophotometry and 1 H‐NMR spectroscopy showed that recombinant Rv1916 does not possess isocitrate lyase activity, while waterLOGSY binding experiments demonstrated that it could bind acetyl‐CoA. Finally, X‐ray crystallography revealed structural similarities between Rv1916 and the C‐terminal domain of ICL2. Considering the probable differences between full‐length ICL2 and the gene products Rv1915 and Rv1916, care must be taken when using Mtb H37Rv as a model organism to study central carbon metabolism." @default.
- W4377194249 created "2023-05-22" @default.
- W4377194249 creator A5016652234 @default.
- W4377194249 creator A5029391932 @default.
- W4377194249 creator A5038064438 @default.
- W4377194249 creator A5046490260 @default.
- W4377194249 creator A5051032153 @default.
- W4377194249 date "2023-06-18" @default.
- W4377194249 modified "2023-10-14" @default.
- W4377194249 title "<i>Mycobacterium tuberculosis</i> Rv1916 is an Acetyl‐CoA‐Binding Protein" @default.
- W4377194249 cites W1209134892 @default.
- W4377194249 cites W1969932115 @default.
- W4377194249 cites W1987805766 @default.
- W4377194249 cites W1989717239 @default.
- W4377194249 cites W2011261850 @default.
- W4377194249 cites W2018765267 @default.
- W4377194249 cites W2019835759 @default.
- W4377194249 cites W2028938598 @default.
- W4377194249 cites W2035226125 @default.
- W4377194249 cites W2047597508 @default.
- W4377194249 cites W2052197954 @default.
- W4377194249 cites W2053867514 @default.
- W4377194249 cites W2059222613 @default.
- W4377194249 cites W2073773613 @default.
- W4377194249 cites W2085792563 @default.
- W4377194249 cites W2099626559 @default.
- W4377194249 cites W2110035145 @default.
- W4377194249 cites W2110808180 @default.
- W4377194249 cites W2122339645 @default.
- W4377194249 cites W2129809239 @default.
- W4377194249 cites W2131417105 @default.
- W4377194249 cites W2144081223 @default.
- W4377194249 cites W2145427045 @default.
- W4377194249 cites W2147874841 @default.
- W4377194249 cites W2154609189 @default.
- W4377194249 cites W2157571601 @default.
- W4377194249 cites W2159211495 @default.
- W4377194249 cites W2163341755 @default.
- W4377194249 cites W2320321097 @default.
- W4377194249 cites W2333655261 @default.
- W4377194249 cites W2598764697 @default.
- W4377194249 cites W2765646020 @default.
- W4377194249 cites W2892337114 @default.
- W4377194249 cites W2972181226 @default.
- W4377194249 cites W2980019106 @default.
- W4377194249 cites W3024136902 @default.
- W4377194249 cites W3029083075 @default.
- W4377194249 cites W3093800357 @default.
- W4377194249 cites W3157217427 @default.
- W4377194249 cites W3170217324 @default.
- W4377194249 cites W4221055315 @default.
- W4377194249 cites W60468736 @default.
- W4377194249 doi "https://doi.org/10.1002/cbic.202300162" @default.
- W4377194249 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37211532" @default.
- W4377194249 hasPublicationYear "2023" @default.
- W4377194249 type Work @default.
- W4377194249 citedByCount "0" @default.
- W4377194249 crossrefType "journal-article" @default.
- W4377194249 hasAuthorship W4377194249A5016652234 @default.
- W4377194249 hasAuthorship W4377194249A5029391932 @default.
- W4377194249 hasAuthorship W4377194249A5038064438 @default.
- W4377194249 hasAuthorship W4377194249A5046490260 @default.
- W4377194249 hasAuthorship W4377194249A5051032153 @default.
- W4377194249 hasBestOaLocation W43771942491 @default.
- W4377194249 hasConcept C104317684 @default.
- W4377194249 hasConcept C142724271 @default.
- W4377194249 hasConcept C165267168 @default.
- W4377194249 hasConcept C181199279 @default.
- W4377194249 hasConcept C185592680 @default.
- W4377194249 hasConcept C2776336597 @default.
- W4377194249 hasConcept C2777269803 @default.
- W4377194249 hasConcept C2777975735 @default.
- W4377194249 hasConcept C2781069245 @default.
- W4377194249 hasConcept C29906990 @default.
- W4377194249 hasConcept C40767141 @default.
- W4377194249 hasConcept C501734568 @default.
- W4377194249 hasConcept C53345823 @default.
- W4377194249 hasConcept C55493867 @default.
- W4377194249 hasConcept C71924100 @default.
- W4377194249 hasConcept C86803240 @default.
- W4377194249 hasConceptScore W4377194249C104317684 @default.
- W4377194249 hasConceptScore W4377194249C142724271 @default.
- W4377194249 hasConceptScore W4377194249C165267168 @default.
- W4377194249 hasConceptScore W4377194249C181199279 @default.
- W4377194249 hasConceptScore W4377194249C185592680 @default.
- W4377194249 hasConceptScore W4377194249C2776336597 @default.
- W4377194249 hasConceptScore W4377194249C2777269803 @default.
- W4377194249 hasConceptScore W4377194249C2777975735 @default.
- W4377194249 hasConceptScore W4377194249C2781069245 @default.
- W4377194249 hasConceptScore W4377194249C29906990 @default.
- W4377194249 hasConceptScore W4377194249C40767141 @default.
- W4377194249 hasConceptScore W4377194249C501734568 @default.
- W4377194249 hasConceptScore W4377194249C53345823 @default.
- W4377194249 hasConceptScore W4377194249C55493867 @default.
- W4377194249 hasConceptScore W4377194249C71924100 @default.
- W4377194249 hasConceptScore W4377194249C86803240 @default.
- W4377194249 hasFunder F4320320974 @default.
- W4377194249 hasFunder F4320334749 @default.
- W4377194249 hasIssue "14" @default.
- W4377194249 hasLocation W43771942491 @default.