Matches in SemOpenAlex for { <https://semopenalex.org/work/W4377291096> ?p ?o ?g. }
- W4377291096 abstract "Abstract Long-read sequencing technologies have improved significantly since their emergence. Their read lengths, potentially spanning entire transcripts, is advantageous for reconstructing transcriptomes. Existing long-read transcriptome assembly methods are primarily reference-based and to date, there is little focus on reference-free transcriptome assembly. We introduce “RNA-Bloom2 [ https://github.com/bcgsc/RNA-Bloom ]”, a reference-free assembly method for long-read transcriptome sequencing data. Using simulated datasets and spike-in control data, we show that the transcriptome assembly quality of RNA-Bloom2 is competitive to those of reference-based methods. Furthermore, we find that RNA-Bloom2 requires 27.0 to 80.6% of the peak memory and 3.6 to 10.8% of the total wall-clock runtime of a competing reference-free method. Finally, we showcase RNA-Bloom2 in assembling a transcriptome sample of Picea sitchensis (Sitka spruce). Since our method does not rely on a reference, it further sets the groundwork for large-scale comparative transcriptomics where high-quality draft genome assemblies are not readily available." @default.
- W4377291096 created "2023-05-23" @default.
- W4377291096 creator A5005018805 @default.
- W4377291096 creator A5017539699 @default.
- W4377291096 creator A5027259274 @default.
- W4377291096 creator A5038133198 @default.
- W4377291096 creator A5053859333 @default.
- W4377291096 creator A5054392207 @default.
- W4377291096 creator A5071511946 @default.
- W4377291096 date "2023-05-22" @default.
- W4377291096 modified "2023-10-14" @default.
- W4377291096 title "Reference-free assembly of long-read transcriptome sequencing data with RNA-Bloom2" @default.
- W4377291096 cites W2011657487 @default.
- W4377291096 cites W2018274843 @default.
- W4377291096 cites W2042160423 @default.
- W4377291096 cites W2045253069 @default.
- W4377291096 cites W2054524196 @default.
- W4377291096 cites W2079564382 @default.
- W4377291096 cites W2102461176 @default.
- W4377291096 cites W2108108770 @default.
- W4377291096 cites W2131271579 @default.
- W4377291096 cites W2168645015 @default.
- W4377291096 cites W2169456326 @default.
- W4377291096 cites W2233603983 @default.
- W4377291096 cites W2480207604 @default.
- W4377291096 cites W2536217010 @default.
- W4377291096 cites W2550586733 @default.
- W4377291096 cites W2575750030 @default.
- W4377291096 cites W2583377988 @default.
- W4377291096 cites W2587686048 @default.
- W4377291096 cites W2751172748 @default.
- W4377291096 cites W2789843538 @default.
- W4377291096 cites W2890229885 @default.
- W4377291096 cites W2899600207 @default.
- W4377291096 cites W2924285454 @default.
- W4377291096 cites W2936594259 @default.
- W4377291096 cites W2950063384 @default.
- W4377291096 cites W2950891420 @default.
- W4377291096 cites W2952615074 @default.
- W4377291096 cites W2952666313 @default.
- W4377291096 cites W2980539212 @default.
- W4377291096 cites W2992976869 @default.
- W4377291096 cites W2995527562 @default.
- W4377291096 cites W3007172120 @default.
- W4377291096 cites W3011428862 @default.
- W4377291096 cites W3033216646 @default.
- W4377291096 cites W3064807468 @default.
- W4377291096 cites W3112376646 @default.
- W4377291096 cites W3119188679 @default.
- W4377291096 cites W3171864942 @default.
- W4377291096 cites W3186309583 @default.
- W4377291096 cites W3191132797 @default.
- W4377291096 cites W3206657624 @default.
- W4377291096 cites W4200262120 @default.
- W4377291096 cites W4231756251 @default.
- W4377291096 cites W4283807461 @default.
- W4377291096 cites W4284885445 @default.
- W4377291096 cites W4292326876 @default.
- W4377291096 cites W4377291096 @default.
- W4377291096 doi "https://doi.org/10.1038/s41467-023-38553-y" @default.
- W4377291096 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37217540" @default.
- W4377291096 hasPublicationYear "2023" @default.
- W4377291096 type Work @default.
- W4377291096 citedByCount "4" @default.
- W4377291096 countsByYear W43772910962023 @default.
- W4377291096 crossrefType "journal-article" @default.
- W4377291096 hasAuthorship W4377291096A5005018805 @default.
- W4377291096 hasAuthorship W4377291096A5017539699 @default.
- W4377291096 hasAuthorship W4377291096A5027259274 @default.
- W4377291096 hasAuthorship W4377291096A5038133198 @default.
- W4377291096 hasAuthorship W4377291096A5053859333 @default.
- W4377291096 hasAuthorship W4377291096A5054392207 @default.
- W4377291096 hasAuthorship W4377291096A5071511946 @default.
- W4377291096 hasBestOaLocation W43772910961 @default.
- W4377291096 hasConcept C104317684 @default.
- W4377291096 hasConcept C107397762 @default.
- W4377291096 hasConcept C141231307 @default.
- W4377291096 hasConcept C150194340 @default.
- W4377291096 hasConcept C162317418 @default.
- W4377291096 hasConcept C18949551 @default.
- W4377291096 hasConcept C192953774 @default.
- W4377291096 hasConcept C41008148 @default.
- W4377291096 hasConcept C50489715 @default.
- W4377291096 hasConcept C54355233 @default.
- W4377291096 hasConcept C67705224 @default.
- W4377291096 hasConcept C70721500 @default.
- W4377291096 hasConcept C86803240 @default.
- W4377291096 hasConceptScore W4377291096C104317684 @default.
- W4377291096 hasConceptScore W4377291096C107397762 @default.
- W4377291096 hasConceptScore W4377291096C141231307 @default.
- W4377291096 hasConceptScore W4377291096C150194340 @default.
- W4377291096 hasConceptScore W4377291096C162317418 @default.
- W4377291096 hasConceptScore W4377291096C18949551 @default.
- W4377291096 hasConceptScore W4377291096C192953774 @default.
- W4377291096 hasConceptScore W4377291096C41008148 @default.
- W4377291096 hasConceptScore W4377291096C50489715 @default.
- W4377291096 hasConceptScore W4377291096C54355233 @default.
- W4377291096 hasConceptScore W4377291096C67705224 @default.
- W4377291096 hasConceptScore W4377291096C70721500 @default.
- W4377291096 hasConceptScore W4377291096C86803240 @default.