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- W4377293773 endingPage "213480" @default.
- W4377293773 startingPage "213480" @default.
- W4377293773 abstract "Research aimed at preserving female fertility is increasingly using bioengineering techniques to develop new platforms capable of supporting ovarian cell function in vitro and in vivo. Natural hydrogels (alginate, collagen, and fibrin) have been the most exploited approaches; however they are biologically inert and/or biochemically simple. Thus, establishing a suitable biomimetic hydrogel from decellularized ovarian cortex (OC) extracellular matrix (OvaECM) could provide a complex native biomaterial for follicle development and oocyte maturation. The objectives of this work were (i) to establish an optimal protocol to decellularize and solubilize bovine OC, (ii) to characterize the histological, molecular, ultrastructural, and proteomic properties of the resulting tissue and hydrogel, and (iii) to assess its biocompatibility and adequacy for murine in vitro follicle growth (IVFG). Sodium dodecyl sulfate was identified as the best detergent to develop bovine OvaECM hydrogels. Hydrogels added into standard media or used as plate coatings were employed for IVFG and oocyte maturation. Follicle growth, survival, hormone production, and oocyte maturation and developmental competence were evaluated. OvaECM hydrogel-supplemented media best supported follicle survival, expansion, and hormone production, while the coatings provided more mature and competent oocytes. Overall, the findings support the xenogeneic use of OvaECM hydrogels for future human female reproductive bioengineering." @default.
- W4377293773 created "2023-05-23" @default.
- W4377293773 creator A5009668029 @default.
- W4377293773 creator A5010738992 @default.
- W4377293773 creator A5014138630 @default.
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- W4377293773 creator A5022881783 @default.
- W4377293773 creator A5037102206 @default.
- W4377293773 creator A5070743974 @default.
- W4377293773 creator A5091600128 @default.
- W4377293773 date "2023-08-01" @default.
- W4377293773 modified "2023-09-23" @default.
- W4377293773 title "Advances of xenogeneic ovarian extracellular matrix hydrogels for in vitro follicle development and oocyte maturation" @default.
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