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• W4377693608 abstract "We attempt to investigate the expression pattern of GDF11 in the sciatic nerves after injury.Thirty-six healthy male Sprague Dawley (SD) rats were divided into three groups at random and were labelled as: day 1, day 4, and day 7 post-surgery. The sciatic nerve crush model was established on the left-hind limb, while the right limb was untreated, and served as the control. Nerve samples were collected at post-injury day 1, day 4 and day 7. Nerve samples collected from the proximal and distal stump of the injury site underwent immunofluorescence staining with GDF11, NF200 and CD31. GDF11 mRNA expression was analyzed by qRT-PCR. CCK-8 assay, after si-GDF11 transfection in Schwann cells (RSC96) was applied to verify its effect in cell proliferation rate.GDF11 was abundantly expressed in axons stained with NF200 and Schwann cells stained with S100. However, no GDF11 expression was observed in vascular endothelial tissues stained with CD31. From day 4 onwards, the level of GDF11 showed an increasing trend, up to a twofold level at day 7 after injury. Proliferation rate of RSC96 cells showed a significant decrease after the down-regulation of GDF11 by siRNAs compared to the control group.GDF11 may play a role in the proliferation of Schwann cell during nerve regeneration process." @default.
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• W4377693608 date "2023-05-23" @default.
• W4377693608 modified "2023-10-11" @default.
• W4377693608 title "Effect of growth differentiation factor 11 expression after peripheral nerve injury in Sprague-Dawley rats" @default.
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• W4377693608 doi "https://doi.org/10.1080/01616412.2023.2211446" @default.
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