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• W4377940852 abstract "Promiscuous enzymes show great potential to establish new-to-nature pathways and expand chemical diversity. Enzyme engineering strategies are often employed to tailor such enzymes to improve their activity or specificity. It is paramount to identify the target residues to be mutated. Here, by exploring the inactivation mechanism with the aid of mass spectrometry, we have identified and mutated critical residues at the dimer interface region of the promiscuous methyltransferase (pMT) that converts psi-ionone to irone. The optimized pMT12 mutant showed ∼1.6–4.8-fold higher kcat than the previously reported best mutant, pMT10, and increased the cis-α-irone percentage from ∼70 to ∼83%. By one-step biotransformation, ∼121.8 mg L–1 cis-α-irone was produced from psi-ionone by the pMT12 mutant. The study offers new opportunities to engineer enzymes with enhanced activity and specificity." @default.
• W4377940852 created "2023-05-25" @default.
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• W4377940852 date "2023-05-23" @default.
• W4377940852 modified "2023-10-03" @default.
• W4377940852 title "Mutagenesis of Dimer Interfacial Residues Improves the Activity and Specificity of Methyltransferase for <i>cis</i>-α-Irone Biosynthesis" @default.
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• W4377940852 doi "https://doi.org/10.1021/acs.jafc.3c01272" @default.
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