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- W4377967666 abstract "Extracellular matrix (ECM) in the human tissue contains vesicles, which are defined as matrix-bound nanovesicles (MBVs). MBVs serve as one of the functional components in ECM, recapitulating part of the regulatory roles and in vivo microenvironment. In this study, extracellular vesicles from culture supernatants (SuEVs) and MBVs are isolated from the conditioned medium or ECM, respectively, of 3D human mesenchymal stem cells. Nanoparticle tracking analysis shows that MBVs are smaller than SuEVs (100-150 nm). Transmission electron microscopy captures the typical cup shape morphology for both SuEVs and MBVs. Western blot reveals that MBVs have low detection of some SuEV markers such as syntenin-1. miRNA analysis of MBVs shows that 3D microenvironment enhances the expression of miRNAs such as miR-19a and miR-21. In vitro functional analysis shows that MBVs can facilitate human pluripotent stem cell-derived forebrain organoid recovery after starvation and promote high passage fibroblast proliferation. In macrophage polarization, 2D MBVs tend to suppress the pro-inflammatory cytokine IL-12β, while 3D MBVs tend to enhance the anti-inflammatory cytokine IL-10. This study has the significance in advancing the understanding of the bio-interface of nanovesicles with human tissue and the design of cell-free therapy for treating neurological disorders such as ischemic stroke." @default.
- W4377967666 created "2023-05-25" @default.
- W4377967666 creator A5003171597 @default.
- W4377967666 creator A5011871830 @default.
- W4377967666 creator A5015283451 @default.
- W4377967666 creator A5041764614 @default.
- W4377967666 creator A5046214153 @default.
- W4377967666 creator A5046610531 @default.
- W4377967666 creator A5057255120 @default.
- W4377967666 creator A5063691131 @default.
- W4377967666 date "2023-06-04" @default.
- W4377967666 modified "2023-10-17" @default.
- W4377967666 title "Engineering Extracellular Matrix‐Bound Nanovesicles Secreted by Three‐Dimensional Human Mesenchymal Stem Cells" @default.
- W4377967666 cites W1646577443 @default.
- W4377967666 cites W1955594488 @default.
- W4377967666 cites W1970178087 @default.
- W4377967666 cites W1970368333 @default.
- W4377967666 cites W1973763077 @default.
- W4377967666 cites W1979493465 @default.
- W4377967666 cites W1983931097 @default.
- W4377967666 cites W1984807833 @default.
- W4377967666 cites W1993527510 @default.
- W4377967666 cites W1995030818 @default.
- W4377967666 cites W1996428696 @default.
- W4377967666 cites W2003984619 @default.
- W4377967666 cites W2030028037 @default.
- W4377967666 cites W2032735323 @default.
- W4377967666 cites W2034251280 @default.
- W4377967666 cites W2045295282 @default.
- W4377967666 cites W2055424631 @default.
- W4377967666 cites W2063756587 @default.
- W4377967666 cites W2089723196 @default.
- W4377967666 cites W2103211592 @default.
- W4377967666 cites W2105864938 @default.
- W4377967666 cites W2122552669 @default.
- W4377967666 cites W2128095876 @default.
- W4377967666 cites W2128585463 @default.
- W4377967666 cites W2155044423 @default.
- W4377967666 cites W2157559660 @default.
- W4377967666 cites W2221267861 @default.
- W4377967666 cites W2285686547 @default.
- W4377967666 cites W2319152885 @default.
- W4377967666 cites W2323044652 @default.
- W4377967666 cites W2334127123 @default.
- W4377967666 cites W2417862864 @default.
- W4377967666 cites W2463753610 @default.
- W4377967666 cites W2463806122 @default.
- W4377967666 cites W2510108534 @default.
- W4377967666 cites W2539719512 @default.
- W4377967666 cites W2546877613 @default.
- W4377967666 cites W2566923366 @default.
- W4377967666 cites W2587206126 @default.
- W4377967666 cites W2592818591 @default.
- W4377967666 cites W2607766702 @default.
- W4377967666 cites W2620850508 @default.
- W4377967666 cites W2626725994 @default.
- W4377967666 cites W2753884258 @default.
- W4377967666 cites W2762018708 @default.
- W4377967666 cites W2766366345 @default.
- W4377967666 cites W2787211370 @default.
- W4377967666 cites W2805892376 @default.
- W4377967666 cites W2805905907 @default.
- W4377967666 cites W2808912161 @default.
- W4377967666 cites W2887936357 @default.
- W4377967666 cites W2891735879 @default.
- W4377967666 cites W2903201035 @default.
- W4377967666 cites W2911048898 @default.
- W4377967666 cites W2911991236 @default.
- W4377967666 cites W2912148636 @default.
- W4377967666 cites W2919318157 @default.
- W4377967666 cites W2935703272 @default.
- W4377967666 cites W2964321516 @default.
- W4377967666 cites W2967982964 @default.
- W4377967666 cites W2971022014 @default.
- W4377967666 cites W2973824501 @default.
- W4377967666 cites W2989661489 @default.
- W4377967666 cites W2989687490 @default.
- W4377967666 cites W2991674051 @default.
- W4377967666 cites W2992079273 @default.
- W4377967666 cites W3000985212 @default.
- W4377967666 cites W3002971935 @default.
- W4377967666 cites W3003703443 @default.
- W4377967666 cites W3009137580 @default.
- W4377967666 cites W3011362237 @default.
- W4377967666 cites W3013565710 @default.
- W4377967666 cites W3018967490 @default.
- W4377967666 cites W3038019273 @default.
- W4377967666 cites W3043045961 @default.
- W4377967666 cites W3049074885 @default.
- W4377967666 cites W3080395658 @default.
- W4377967666 cites W3081011277 @default.
- W4377967666 cites W3087199948 @default.
- W4377967666 cites W3087797540 @default.
- W4377967666 cites W3087999199 @default.
- W4377967666 cites W3089777907 @default.
- W4377967666 cites W3107587686 @default.
- W4377967666 cites W3115923592 @default.
- W4377967666 cites W3125322167 @default.
- W4377967666 cites W3126965852 @default.
- W4377967666 cites W3127063032 @default.