Matches in SemOpenAlex for { <https://semopenalex.org/work/W4378070511> ?p ?o ?g. }
- W4378070511 abstract "The ERM (ezrin, radixin, and moesin) family of proteins and the related protein merlin participate in scaffolding and signaling events at the cell cortex. The proteins share an N-terminal FERM [band four-point-one (4.1) ERM] domain composed of three subdomains (F1, F2, and F3) with binding sites for short linear peptide motifs. By screening the FERM domains of the ERMs and merlin against a phage library that displays peptides representing the intrinsically disordered regions of the human proteome, we identified a large number of novel ligands. We determined the affinities for the ERM and merlin FERM domains interacting with 18 peptides and validated interactions with full-length proteins through pull-down experiments. The majority of the peptides contained an apparent Yx[FILV] motif; others show alternative motifs. We defined distinct binding sites for two types of similar but distinct binding motifs (YxV and FYDF) using a combination of Rosetta FlexPepDock computational peptide docking protocols and mutational analysis. We provide a detailed molecular understanding of how the two types of peptides with distinct motifs bind to different sites on the moesin FERM phosphotyrosine binding-like subdomain and uncover interdependencies between the different types of ligands. The study expands the motif-based interactomes of the ERMs and merlin and suggests that the FERM domain acts as a switchable interaction hub." @default.
- W4378070511 created "2023-05-25" @default.
- W4378070511 creator A5020647941 @default.
- W4378070511 creator A5034150494 @default.
- W4378070511 creator A5067140972 @default.
- W4378070511 creator A5075869401 @default.
- W4378070511 creator A5080994466 @default.
- W4378070511 date "2023-05-24" @default.
- W4378070511 modified "2023-10-17" @default.
- W4378070511 title "Elucidation of Short Linear Motif-Based Interactions of the FERM Domains of Ezrin, Radixin, Moesin, and Merlin" @default.
- W4378070511 cites W1517212026 @default.
- W4378070511 cites W1804190961 @default.
- W4378070511 cites W1847717811 @default.
- W4378070511 cites W1963587413 @default.
- W4378070511 cites W1965831400 @default.
- W4378070511 cites W1969884064 @default.
- W4378070511 cites W1976104845 @default.
- W4378070511 cites W1983102499 @default.
- W4378070511 cites W1985531875 @default.
- W4378070511 cites W1987721851 @default.
- W4378070511 cites W1988472239 @default.
- W4378070511 cites W1989372844 @default.
- W4378070511 cites W1999390565 @default.
- W4378070511 cites W1999648327 @default.
- W4378070511 cites W2005252392 @default.
- W4378070511 cites W2005679420 @default.
- W4378070511 cites W2007909109 @default.
- W4378070511 cites W2010994824 @default.
- W4378070511 cites W2025013613 @default.
- W4378070511 cites W2032825057 @default.
- W4378070511 cites W2036854810 @default.
- W4378070511 cites W2037617680 @default.
- W4378070511 cites W2038505563 @default.
- W4378070511 cites W2039771278 @default.
- W4378070511 cites W2042015318 @default.
- W4378070511 cites W2044145005 @default.
- W4378070511 cites W2051579741 @default.
- W4378070511 cites W2058910296 @default.
- W4378070511 cites W2065839189 @default.
- W4378070511 cites W2076430025 @default.
- W4378070511 cites W2079960371 @default.
- W4378070511 cites W2081460564 @default.
- W4378070511 cites W2091559752 @default.
- W4378070511 cites W2105193974 @default.
- W4378070511 cites W2106477324 @default.
- W4378070511 cites W2112981236 @default.
- W4378070511 cites W2115799757 @default.
- W4378070511 cites W2116890528 @default.
- W4378070511 cites W2135815512 @default.
- W4378070511 cites W2157000115 @default.
- W4378070511 cites W2159488271 @default.
- W4378070511 cites W2289124515 @default.
- W4378070511 cites W2564675054 @default.
- W4378070511 cites W2789251983 @default.
- W4378070511 cites W2795560340 @default.
- W4378070511 cites W2940877739 @default.
- W4378070511 cites W2952059807 @default.
- W4378070511 cites W3015964336 @default.
- W4378070511 cites W3085129072 @default.
- W4378070511 cites W3136241276 @default.
- W4378070511 cites W3157357317 @default.
- W4378070511 cites W4205989657 @default.
- W4378070511 cites W4244527422 @default.
- W4378070511 doi "https://doi.org/10.1021/acs.biochem.3c00096" @default.
- W4378070511 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37224425" @default.
- W4378070511 hasPublicationYear "2023" @default.
- W4378070511 type Work @default.
- W4378070511 citedByCount "2" @default.
- W4378070511 countsByYear W43780705112023 @default.
- W4378070511 crossrefType "journal-article" @default.
- W4378070511 hasAuthorship W4378070511A5020647941 @default.
- W4378070511 hasAuthorship W4378070511A5034150494 @default.
- W4378070511 hasAuthorship W4378070511A5067140972 @default.
- W4378070511 hasAuthorship W4378070511A5075869401 @default.
- W4378070511 hasAuthorship W4378070511A5080994466 @default.
- W4378070511 hasBestOaLocation W43780705111 @default.
- W4378070511 hasConcept C104317684 @default.
- W4378070511 hasConcept C132677234 @default.
- W4378070511 hasConcept C142669718 @default.
- W4378070511 hasConcept C144647389 @default.
- W4378070511 hasConcept C1491633281 @default.
- W4378070511 hasConcept C161200384 @default.
- W4378070511 hasConcept C178628643 @default.
- W4378070511 hasConcept C179185449 @default.
- W4378070511 hasConcept C185592680 @default.
- W4378070511 hasConcept C196509110 @default.
- W4378070511 hasConcept C200342125 @default.
- W4378070511 hasConcept C2776292417 @default.
- W4378070511 hasConcept C2780246058 @default.
- W4378070511 hasConcept C41625074 @default.
- W4378070511 hasConcept C51639874 @default.
- W4378070511 hasConcept C54355233 @default.
- W4378070511 hasConcept C55493867 @default.
- W4378070511 hasConcept C70721500 @default.
- W4378070511 hasConcept C86803240 @default.
- W4378070511 hasConcept C95444343 @default.
- W4378070511 hasConceptScore W4378070511C104317684 @default.
- W4378070511 hasConceptScore W4378070511C132677234 @default.
- W4378070511 hasConceptScore W4378070511C142669718 @default.
- W4378070511 hasConceptScore W4378070511C144647389 @default.