Matches in SemOpenAlex for { <https://semopenalex.org/work/W4378072364> ?p ?o ?g. }
- W4378072364 abstract "Local substrate stiffness is one of the major mechanical inputs for tissue organization during its development and remodeling. It is widely recognized that adherent cells use transmembrane proteins (integrins) at focal adhesions to translate ECM mechanical cues into intracellular bioprocess. Here we show that epithelial cells respond to substrate stiffening primarily via actin cytoskeleton organization, that requires activation of mechanosensitive Piezo1 channels. Piezo1 Knockdown cells eliminated the actin stress fibers that formed on stiff substrates, while it had minimal effect on cell morphology and spreading area. Inhibition of Piezo1 channels with GsMTx4 also significantly reduced stiffness-induced F-actin reorganization, suggesting Piezo1 mediated cation current plays a role. Activation of Piezo1 channels with specific agonist (Yoda1) resulted in thickening of F-actin fibers and enlargement of FAs on stiffer substrates, whereas it did not affect the formation of nascent FAs that facilitate spreading on the soft substrates. These results demonstrate that Piezo1 functions as a force sensor that couples with actin cytoskeleton to distinguish the substrate stiffness and facilitate epithelial adaptive remodeling." @default.
- W4378072364 created "2023-05-25" @default.
- W4378072364 creator A5002224948 @default.
- W4378072364 creator A5015549454 @default.
- W4378072364 creator A5025676751 @default.
- W4378072364 date "2023-05-24" @default.
- W4378072364 modified "2023-10-01" @default.
- W4378072364 title "Epithelial cells sense local stiffness via Piezo1 mediated cytoskeletal reorganization" @default.
- W4378072364 cites W1966501485 @default.
- W4378072364 cites W1972071814 @default.
- W4378072364 cites W1972974984 @default.
- W4378072364 cites W1973089587 @default.
- W4378072364 cites W1979069664 @default.
- W4378072364 cites W1993006648 @default.
- W4378072364 cites W1999720993 @default.
- W4378072364 cites W2004199264 @default.
- W4378072364 cites W2024250498 @default.
- W4378072364 cites W2032647105 @default.
- W4378072364 cites W2054635074 @default.
- W4378072364 cites W2057846852 @default.
- W4378072364 cites W2058738554 @default.
- W4378072364 cites W2060934353 @default.
- W4378072364 cites W2063208689 @default.
- W4378072364 cites W2074572112 @default.
- W4378072364 cites W2086822491 @default.
- W4378072364 cites W2104451738 @default.
- W4378072364 cites W2112924183 @default.
- W4378072364 cites W2116517871 @default.
- W4378072364 cites W2125644189 @default.
- W4378072364 cites W2141075109 @default.
- W4378072364 cites W2148328620 @default.
- W4378072364 cites W2166599924 @default.
- W4378072364 cites W2588123864 @default.
- W4378072364 cites W2752163435 @default.
- W4378072364 cites W2804041046 @default.
- W4378072364 cites W2896032894 @default.
- W4378072364 cites W2944340502 @default.
- W4378072364 cites W2953531477 @default.
- W4378072364 cites W2966539065 @default.
- W4378072364 cites W2968420254 @default.
- W4378072364 cites W2999312392 @default.
- W4378072364 cites W3135728918 @default.
- W4378072364 cites W3137728895 @default.
- W4378072364 cites W3165135090 @default.
- W4378072364 cites W4221000110 @default.
- W4378072364 cites W4308648843 @default.
- W4378072364 cites W576850159 @default.
- W4378072364 doi "https://doi.org/10.3389/fcell.2023.1198109" @default.
- W4378072364 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37293127" @default.
- W4378072364 hasPublicationYear "2023" @default.
- W4378072364 type Work @default.
- W4378072364 citedByCount "2" @default.
- W4378072364 countsByYear W43780723642023 @default.
- W4378072364 crossrefType "journal-article" @default.
- W4378072364 hasAuthorship W4378072364A5002224948 @default.
- W4378072364 hasAuthorship W4378072364A5015549454 @default.
- W4378072364 hasAuthorship W4378072364A5025676751 @default.
- W4378072364 hasBestOaLocation W43780723641 @default.
- W4378072364 hasConcept C12554922 @default.
- W4378072364 hasConcept C125705527 @default.
- W4378072364 hasConcept C142669718 @default.
- W4378072364 hasConcept C1491633281 @default.
- W4378072364 hasConcept C151166631 @default.
- W4378072364 hasConcept C170493617 @default.
- W4378072364 hasConcept C181104049 @default.
- W4378072364 hasConcept C185592680 @default.
- W4378072364 hasConcept C195687474 @default.
- W4378072364 hasConcept C2780785647 @default.
- W4378072364 hasConcept C2781099445 @default.
- W4378072364 hasConcept C2993400109 @default.
- W4378072364 hasConcept C3620293 @default.
- W4378072364 hasConcept C4001165 @default.
- W4378072364 hasConcept C50254741 @default.
- W4378072364 hasConcept C55493867 @default.
- W4378072364 hasConcept C62478195 @default.
- W4378072364 hasConcept C86803240 @default.
- W4378072364 hasConcept C95444343 @default.
- W4378072364 hasConceptScore W4378072364C12554922 @default.
- W4378072364 hasConceptScore W4378072364C125705527 @default.
- W4378072364 hasConceptScore W4378072364C142669718 @default.
- W4378072364 hasConceptScore W4378072364C1491633281 @default.
- W4378072364 hasConceptScore W4378072364C151166631 @default.
- W4378072364 hasConceptScore W4378072364C170493617 @default.
- W4378072364 hasConceptScore W4378072364C181104049 @default.
- W4378072364 hasConceptScore W4378072364C185592680 @default.
- W4378072364 hasConceptScore W4378072364C195687474 @default.
- W4378072364 hasConceptScore W4378072364C2780785647 @default.
- W4378072364 hasConceptScore W4378072364C2781099445 @default.
- W4378072364 hasConceptScore W4378072364C2993400109 @default.
- W4378072364 hasConceptScore W4378072364C3620293 @default.
- W4378072364 hasConceptScore W4378072364C4001165 @default.
- W4378072364 hasConceptScore W4378072364C50254741 @default.
- W4378072364 hasConceptScore W4378072364C55493867 @default.
- W4378072364 hasConceptScore W4378072364C62478195 @default.
- W4378072364 hasConceptScore W4378072364C86803240 @default.
- W4378072364 hasConceptScore W4378072364C95444343 @default.
- W4378072364 hasFunder F4320332167 @default.
- W4378072364 hasLocation W43780723641 @default.
- W4378072364 hasLocation W43780723642 @default.
- W4378072364 hasLocation W43780723643 @default.