Matches in SemOpenAlex for { <https://semopenalex.org/work/W4378212837> ?p ?o ?g. }
- W4378212837 abstract "Abstract Aflatoxin M1 (AFM1) is a mycotoxin that is commonly found as a milk contaminant, and its presence in milk has been linked to cytotoxicity. The present study aimed to evaluate the acute cytotoxic effects of AFM1 on intestinal CaCo2 cells. Initially, we checked the morphology and viability of CaCo2 cells after treatment with different concentrations of AFM1 (5ng/L, 50ng/L, 250ng/L, 500ng/L, 1000ng/L, and 2000ng/L) for different time intervals (6hrs, 12hrs, and 24hrs). It was found that AFM1 didn’t show any effect on cell morphology and viability. Further, DCFDA assay showed increased ROS production after 6hrs treatments. qPCR analysis showed an increased expression of epithelial specific cytoskeleton markers Cytokeratin, Villin, Vimentin, and JAM1, and a decreased expression of tight junction proteins, Claudin, Occludin, and ZO1. Similarly, we found an increased expression of Cyp1a1 transcript with an increasing AFM1 concentration and incubation time. This gene expression analysis showed AFM1 can causes disruption of gap junctions between intestinal cells, which was further confirmed by a transwell experiment. In conclusion, consumption of AFM1-contaminated milk doesn’t show any effect on cells morphology and viability but decreases the expression of intestinal barrier transcripts that may leads to the disruption of intestinal barrier function and leaky gut." @default.
- W4378212837 created "2023-05-26" @default.
- W4378212837 creator A5004691971 @default.
- W4378212837 creator A5006778578 @default.
- W4378212837 creator A5028533649 @default.
- W4378212837 creator A5087966610 @default.
- W4378212837 creator A5092017379 @default.
- W4378212837 creator A5092017380 @default.
- W4378212837 date "2023-05-25" @default.
- W4378212837 modified "2023-10-10" @default.
- W4378212837 title "Aflatoxin M1 causes cytotoxicity and intestinal epithelial cell integrity damage in differentiated human Caco-2 cells" @default.
- W4378212837 cites W1970326574 @default.
- W4378212837 cites W1972908905 @default.
- W4378212837 cites W1974782561 @default.
- W4378212837 cites W1981447874 @default.
- W4378212837 cites W1984056103 @default.
- W4378212837 cites W1997619156 @default.
- W4378212837 cites W1997990251 @default.
- W4378212837 cites W2003244933 @default.
- W4378212837 cites W2005225463 @default.
- W4378212837 cites W2013144741 @default.
- W4378212837 cites W2013355749 @default.
- W4378212837 cites W2014476767 @default.
- W4378212837 cites W2015271858 @default.
- W4378212837 cites W2019117690 @default.
- W4378212837 cites W2024785511 @default.
- W4378212837 cites W2025238816 @default.
- W4378212837 cites W2026677619 @default.
- W4378212837 cites W2027577700 @default.
- W4378212837 cites W2030094683 @default.
- W4378212837 cites W2033507828 @default.
- W4378212837 cites W2039674474 @default.
- W4378212837 cites W2041454039 @default.
- W4378212837 cites W2053693558 @default.
- W4378212837 cites W2056975261 @default.
- W4378212837 cites W2061007824 @default.
- W4378212837 cites W2061583677 @default.
- W4378212837 cites W2062342749 @default.
- W4378212837 cites W2066906832 @default.
- W4378212837 cites W2069276980 @default.
- W4378212837 cites W2072574704 @default.
- W4378212837 cites W2077269873 @default.
- W4378212837 cites W2083715780 @default.
- W4378212837 cites W2094825032 @default.
- W4378212837 cites W2098213136 @default.
- W4378212837 cites W2103831817 @default.
- W4378212837 cites W2106566497 @default.
- W4378212837 cites W2107277218 @default.
- W4378212837 cites W2107601924 @default.
- W4378212837 cites W2112241819 @default.
- W4378212837 cites W2114426273 @default.
- W4378212837 cites W2115928395 @default.
- W4378212837 cites W2124791313 @default.
- W4378212837 cites W2128995604 @default.
- W4378212837 cites W2130438213 @default.
- W4378212837 cites W2147203421 @default.
- W4378212837 cites W2157896512 @default.
- W4378212837 cites W2158926036 @default.
- W4378212837 cites W2160566428 @default.
- W4378212837 cites W2165710952 @default.
- W4378212837 cites W2167311295 @default.
- W4378212837 cites W2311539200 @default.
- W4378212837 cites W2345933514 @default.
- W4378212837 cites W2473357402 @default.
- W4378212837 cites W2493130077 @default.
- W4378212837 cites W2587315110 @default.
- W4378212837 cites W2612118487 @default.
- W4378212837 cites W2743940282 @default.
- W4378212837 cites W2762166908 @default.
- W4378212837 cites W2776943472 @default.
- W4378212837 cites W2802963324 @default.
- W4378212837 cites W2886429353 @default.
- W4378212837 cites W2924154537 @default.
- W4378212837 cites W2978832932 @default.
- W4378212837 cites W3026674770 @default.
- W4378212837 cites W3090499852 @default.
- W4378212837 cites W3109046553 @default.
- W4378212837 cites W3135279852 @default.
- W4378212837 cites W3145217711 @default.
- W4378212837 cites W566284959 @default.
- W4378212837 doi "https://doi.org/10.21203/rs.3.rs-2927109/v1" @default.
- W4378212837 hasPublicationYear "2023" @default.
- W4378212837 type Work @default.
- W4378212837 citedByCount "0" @default.
- W4378212837 crossrefType "posted-content" @default.
- W4378212837 hasAuthorship W4378212837A5004691971 @default.
- W4378212837 hasAuthorship W4378212837A5006778578 @default.
- W4378212837 hasAuthorship W4378212837A5028533649 @default.
- W4378212837 hasAuthorship W4378212837A5087966610 @default.
- W4378212837 hasAuthorship W4378212837A5092017379 @default.
- W4378212837 hasAuthorship W4378212837A5092017380 @default.
- W4378212837 hasBestOaLocation W43782128371 @default.
- W4378212837 hasConcept C109316439 @default.
- W4378212837 hasConcept C129900496 @default.
- W4378212837 hasConcept C1491633281 @default.
- W4378212837 hasConcept C153911025 @default.
- W4378212837 hasConcept C154317977 @default.
- W4378212837 hasConcept C177779419 @default.
- W4378212837 hasConcept C185592680 @default.
- W4378212837 hasConcept C193176779 @default.