FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4378217863> ?p ?o ?g. }

• W4378217863 abstract "Abstract Background With the safety of blood transfusion being a major public health concern, the development of a rapid, sensitive, specific, and cost-effective multiplex PCR assay for simultaneous detection of hepatitis B virus(HBV), hepatitis C virus (HCV), hepatitis E virus (HEV), and Treponema pallidum(T. pallidum) in blood is crucial. Methods Five primer pairs and probes were designed towards conserved regions of target genes and used to establish a one-step pentaplex real-time reverse transcription PCR(qRT-PCR) assay for simultaneous detection of HBV, HCV, HEV, T. pallidum, and RNase P(housekeeping gene), providing sample quality check. The clinical performance of the assay was further determined with 2400 blood samples from blood donors and patients in Zhejiang province, and compared the results with commercial singleplex qPCR and serological assays. Results The 95% limit of detection(LOD) of HBV, HCV, HEV, and T. pallidum were 7.11 copies/µL, 7.65 copies/µL, 8.45 copies/µL, and 9.06 copies/µL, respectively. Moreover, the assay has good specificity and precision. Compared to the singleplex qPCR assay, the novel assay for detecting HBV, HCV, HEV, and T. pallidum presented 100% clinical sensitivity, specificity, and consistency. Several discrepant results between serological and pentaplex qRT-PCR assays were found. Of 2400 blood samples, there were 2(0.08%) HBsAg positive samples, 3(0.13%) anti-HCV positive samples, 29(1.21%) IgM anti-HEV positive samples and 6(0.25%) anti-T. pallidum positive samples proven negative in nucleic acid detection. 1(0.04%) HBV DNA positive sample and 1(0.04%) HEV RNA positive sample were detected negative by serological testing. Conclusions The developed pentaplex qRT-PCR is the first assay on simultaneous, sensitive, specific, and reproducible detection of HBV, HCV, HEV, T. pallidum, and RNase P in a single tube. It could detect pathogens in blood during the window period of infection and is a good tool for effectively screening blood donors and early clinical diagnosis." @default.
• W4378217863 created "2023-05-26" @default.
• W4378217863 creator A5017279981 @default.
• W4378217863 creator A5028179790 @default.
• W4378217863 creator A5029283198 @default.
• W4378217863 creator A5038996247 @default.
• W4378217863 creator A5065999911 @default.
• W4378217863 creator A5074919815 @default.
• W4378217863 creator A5087223588 @default.
• W4378217863 creator A5087891092 @default.
• W4378217863 creator A5090731971 @default.
• W4378217863 date "2023-05-25" @default.
• W4378217863 modified "2023-10-16" @default.
• W4378217863 title "Development and clinical validation of a one-step pentaplex real-time reverse transcription PCR assay for detection of hepatitis virus B, C, E, Treponema pallidum, and a human housekeeping gene" @default.
• W4378217863 cites W1517477959 @default.
• W4378217863 cites W1528208954 @default.
• W4378217863 cites W1630126239 @default.
• W4378217863 cites W1641280389 @default.
• W4378217863 cites W1668688443 @default.
• W4378217863 cites W1851982349 @default.
• W4378217863 cites W1974276707 @default.
• W4378217863 cites W1982595295 @default.
• W4378217863 cites W2008472378 @default.
• W4378217863 cites W2040108618 @default.
• W4378217863 cites W2050031227 @default.
• W4378217863 cites W2077910056 @default.
• W4378217863 cites W2095508741 @default.
• W4378217863 cites W2102444453 @default.
• W4378217863 cites W2132148617 @default.
• W4378217863 cites W2145722906 @default.
• W4378217863 cites W2145893755 @default.
• W4378217863 cites W2165008961 @default.
• W4378217863 cites W2193804949 @default.
• W4378217863 cites W2256445331 @default.
• W4378217863 cites W2259885574 @default.
• W4378217863 cites W2403485205 @default.
• W4378217863 cites W2417638789 @default.
• W4378217863 cites W2508222219 @default.
• W4378217863 cites W2519714911 @default.
• W4378217863 cites W2770471954 @default.
• W4378217863 cites W2799554049 @default.
• W4378217863 cites W2867789798 @default.
• W4378217863 cites W2891485163 @default.
• W4378217863 cites W2904691036 @default.
• W4378217863 cites W2953180015 @default.
• W4378217863 cites W2972092659 @default.
• W4378217863 cites W3005161839 @default.
• W4378217863 cites W3008614074 @default.
• W4378217863 cites W3037303032 @default.
• W4378217863 cites W3037892832 @default.
• W4378217863 cites W3080566630 @default.
• W4378217863 cites W3149855733 @default.
• W4378217863 cites W4245997385 @default.
• W4378217863 cites W4283588489 @default.
• W4378217863 doi "https://doi.org/10.1186/s12879-023-08240-w" @default.
• W4378217863 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37231355" @default.
• W4378217863 hasPublicationYear "2023" @default.
• W4378217863 type Work @default.
• W4378217863 citedByCount "0" @default.
• W4378217863 crossrefType "journal-article" @default.
• W4378217863 hasAuthorship W4378217863A5017279981 @default.
• W4378217863 hasAuthorship W4378217863A5028179790 @default.
• W4378217863 hasAuthorship W4378217863A5029283198 @default.
• W4378217863 hasAuthorship W4378217863A5038996247 @default.
• W4378217863 hasAuthorship W4378217863A5065999911 @default.
• W4378217863 hasAuthorship W4378217863A5074919815 @default.
• W4378217863 hasAuthorship W4378217863A5087223588 @default.
• W4378217863 hasAuthorship W4378217863A5087891092 @default.
• W4378217863 hasAuthorship W4378217863A5090731971 @default.
• W4378217863 hasBestOaLocation W43782178631 @default.
• W4378217863 hasConcept C159047783 @default.
• W4378217863 hasConcept C159654299 @default.
• W4378217863 hasConcept C203014093 @default.
• W4378217863 hasConcept C2522874641 @default.
• W4378217863 hasConcept C2776983459 @default.
• W4378217863 hasConcept C2777410769 @default.
• W4378217863 hasConcept C2780187896 @default.
• W4378217863 hasConcept C2780593183 @default.
• W4378217863 hasConcept C2781188995 @default.
• W4378217863 hasConcept C3013748606 @default.
• W4378217863 hasConcept C45189115 @default.
• W4378217863 hasConcept C60644358 @default.
• W4378217863 hasConcept C86803240 @default.
• W4378217863 hasConceptScore W4378217863C159047783 @default.
• W4378217863 hasConceptScore W4378217863C159654299 @default.
• W4378217863 hasConceptScore W4378217863C203014093 @default.
• W4378217863 hasConceptScore W4378217863C2522874641 @default.
• W4378217863 hasConceptScore W4378217863C2776983459 @default.
• W4378217863 hasConceptScore W4378217863C2777410769 @default.
• W4378217863 hasConceptScore W4378217863C2780187896 @default.
• W4378217863 hasConceptScore W4378217863C2780593183 @default.
• W4378217863 hasConceptScore W4378217863C2781188995 @default.
• W4378217863 hasConceptScore W4378217863C3013748606 @default.
• W4378217863 hasConceptScore W4378217863C45189115 @default.
• W4378217863 hasConceptScore W4378217863C60644358 @default.
• W4378217863 hasConceptScore W4378217863C86803240 @default.
• W4378217863 hasFunder F4320321001 @default.
• W4378217863 hasIssue "1" @default.
• W4378217863 hasLocation W43782178631 @default.
• W4378217863 hasLocation W43782178632 @default.

• next