Matches in SemOpenAlex for { <https://semopenalex.org/work/W4378648977> ?p ?o ?g. }
Showing items 1 to 54 of
54
with 100 items per page.
- W4378648977 abstract "Endotoxins are harmful for many organisms, including those that live in the water. Therefore, it is important to identify effective ways in identifying if they are present in water environments. Research has been conducted to show that Limulus amebocyte lysate (LAL) is an effective way to determine if endotoxins are present. In this study, LAL was used as the main reagent to detect if endotoxins were present in the tanks at the Pritzker Marine Biology Research Center. It is believed that LAL will be able to detect the presence of any endotoxins within the tanks at Pritzker. The first part of the procedure to do this was to collect 5mL of water from each tank at Pritzker. After the LAL reagent was dissolved in LAL Reagent Water, the gel-clot assay was then produced. 0.1mL of the reagent was added to each assay tube. 0.1mL of each product sample or control was transferred into each assay tube, beginning with the negative control and ending with the highest endotoxin concentration. The contents of the tubes were mixed and incubated undisturbed in a 37 C heating block for 60 minutes. After incubation, each tube was examined for gelation. A positive test is defined as the formation of a firm gel capable of maintaining its integrity when the assay tube is inverted 180 degrees. A negative test is characterized by the absence of gel or by the formation of a viscous mass which does not hold when the assay tube is inverted. Endotoxins were detected in an average of 13 tanks at the Pritzker. To expand upon these findings, quantifying how many endotoxins are present in the tanks and what types of endotoxins are present in the tanks will be tested. Biology department funds at the New College of Florida This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process." @default.
- W4378648977 created "2023-05-30" @default.
- W4378648977 creator A5085027764 @default.
- W4378648977 date "2023-05-01" @default.
- W4378648977 modified "2023-09-27" @default.
- W4378648977 title "Using Limulus Amebocyte Lysate (LAL) to discover the presence of endotoxins in the tanks at the Pritzker Marine Biology Research Center" @default.
- W4378648977 doi "https://doi.org/10.1152/physiol.2023.38.s1.5726593" @default.
- W4378648977 hasPublicationYear "2023" @default.
- W4378648977 type Work @default.
- W4378648977 citedByCount "0" @default.
- W4378648977 crossrefType "journal-article" @default.
- W4378648977 hasAuthorship W4378648977A5085027764 @default.
- W4378648977 hasConcept C151730666 @default.
- W4378648977 hasConcept C178790620 @default.
- W4378648977 hasConcept C185592680 @default.
- W4378648977 hasConcept C203014093 @default.
- W4378648977 hasConcept C2776040547 @default.
- W4378648977 hasConcept C2776487282 @default.
- W4378648977 hasConcept C2778754761 @default.
- W4378648977 hasConcept C40875361 @default.
- W4378648977 hasConcept C43617362 @default.
- W4378648977 hasConcept C55493867 @default.
- W4378648977 hasConcept C57409179 @default.
- W4378648977 hasConcept C86803240 @default.
- W4378648977 hasConceptScore W4378648977C151730666 @default.
- W4378648977 hasConceptScore W4378648977C178790620 @default.
- W4378648977 hasConceptScore W4378648977C185592680 @default.
- W4378648977 hasConceptScore W4378648977C203014093 @default.
- W4378648977 hasConceptScore W4378648977C2776040547 @default.
- W4378648977 hasConceptScore W4378648977C2776487282 @default.
- W4378648977 hasConceptScore W4378648977C2778754761 @default.
- W4378648977 hasConceptScore W4378648977C40875361 @default.
- W4378648977 hasConceptScore W4378648977C43617362 @default.
- W4378648977 hasConceptScore W4378648977C55493867 @default.
- W4378648977 hasConceptScore W4378648977C57409179 @default.
- W4378648977 hasConceptScore W4378648977C86803240 @default.
- W4378648977 hasIssue "S1" @default.
- W4378648977 hasLocation W43786489771 @default.
- W4378648977 hasOpenAccess W4378648977 @default.
- W4378648977 hasPrimaryLocation W43786489771 @default.
- W4378648977 hasRelatedWork W1607976719 @default.
- W4378648977 hasRelatedWork W1662958862 @default.
- W4378648977 hasRelatedWork W1992289738 @default.
- W4378648977 hasRelatedWork W2079323523 @default.
- W4378648977 hasRelatedWork W2117661560 @default.
- W4378648977 hasRelatedWork W2164099992 @default.
- W4378648977 hasRelatedWork W2371747769 @default.
- W4378648977 hasRelatedWork W2412168133 @default.
- W4378648977 hasRelatedWork W3010174998 @default.
- W4378648977 hasRelatedWork W2414549945 @default.
- W4378648977 hasVolume "38" @default.
- W4378648977 isParatext "false" @default.
- W4378648977 isRetracted "false" @default.
- W4378648977 workType "article" @default.