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- W4378745034 abstract "Objective: To establish a method for the rapid determination of acetaminophen (APAP) in human plasma by LC-MS/MS. Methods: The plasma samples were extracted by methanol and acetonitrile (1: 1) and purified directly. C(18) column was used for sample separation. The mobile phase were methanol (5 mmol/L ammonium acetate) and water (5 mmol/L ammonium acetate). Samples were analyzed by LC MS/MS with the electrospray ionization multi reaction monitoring (MRM) mode. Results: The calibration curves of APAP was linear in the concentration range of 0~10 mg/L, the correlation coefficient (r) was greater than 0.999 0. The relative standard deviation within and between batches was less than 10%. The recovery rate were 96.81%~101.7%. The detection limit of the method was 0.1 μg/L and the lower limit of quantification was 0.3 μg/L. Conclusion: This method has strong specificity, high sensitivity and reliable determination results. It is suitable for the rapid analysis of clinical plasma samples.目的： 建立一种快速测定人血浆中对乙酰氨基酚（APAP）含量的液相色谱-串联质谱（LC-MS/MS）方法。 方法： 血浆样品经甲醇-乙腈溶液（V/V，1∶1）沉淀蛋白后，以甲醇（5 mmol/L乙酸铵）-水（5 mmol/L乙酸铵）为流动相，经C18色谱柱分离。采用LC-MS/MS电喷雾正离子多反应监测（MRM）模式进行定性定量分析。 结果： 本方法在0~10 mg/L范围内线性良好，相关系数（r）>0.999 0，批间、批内精密度均<10%，加标回收率为96.8%~101.7%，检出限为0.1 mg/L，定量下限为0.3 mg/L。 结论： 本方法特异性强，灵敏度高，测定结果可靠，适用于临床血浆样品的快速分析。." @default.
- W4378745034 created "2023-05-31" @default.
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- W4378745034 date "2023-05-20" @default.
- W4378745034 modified "2023-09-28" @default.
- W4378745034 title "[Rapid determination of acetaminophen in plasma by LC-MS/MS]." @default.
- W4378745034 doi "https://doi.org/10.3760/cma.j.cn121094-20220330-00163" @default.
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