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- W4378801567 abstract "Third-generation sequencing technologies are being increasingly used in microbiome research and this has given rise to new challenges in computational microbiome analysis. Oxford Nanopore's MinION is a portable sequencer that streams data that can be basecalled on-the-fly. Here we give an introduction to the MAIRA software, which is designed to analyze MinION sequencing reads from a microbiome sample, as they are produced in real-time, on a laptop. The software processes reads in batches and updates the presented analysis after each batch. There are two analysis steps: First, protein alignments are calculated to determine which genera might be present in a sample. When strong evidence for a genus is found, then, in a second step, a more detailed analysis is performed by aligning the reads against the proteins of all species in the detected genus. The program presents a detailed analysis of species, antibiotic resistance genes, and virulence factors." @default.
- W4378801567 created "2023-06-01" @default.
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- W4378801567 date "2023-01-01" @default.
- W4378801567 modified "2023-10-17" @default.
- W4378801567 title "MAIRA: Protein-based Analysis of MinION Reads on a Laptop" @default.
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- W4378801567 doi "https://doi.org/10.1007/978-1-0716-3072-3_11" @default.
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