FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4378833691> ?p ?o ?g. }

• W4378833691 abstract "Vascularization plays a critical role in organ maturation and cell type development. Drug discovery, organ mimicry, and ultimately transplantation in a clinical setting thereby hinges on achieving robust vascularization of in vitro engineered organs. Here, focusing on human kidney organoids, we overcome this hurdle by combining an inducible ETS translocation variant 2 ( ETV2 ) human induced pluripotent stem cell (iPSC) line, which directs endothelial fate, with a non-transgenic iPSC line in suspension organoid culture. The resulting human kidney organoids show extensive vascularization by endothelial cells with an identity most closely related to endogenous kidney endothelia. Vascularized organoids also show increased maturation of nephron structures including more mature podocytes with improved marker expression, foot process interdigitation, an associated fenestrated endothelium, and the presence of renin + cells. The creation of an engineered vascular niche capable of improving kidney organoid maturation and cell type complexity is a significant step forward in the path to clinical translation. Furthermore, this approach is orthogonal to native tissue differentiation paths, hence readily adaptable to other organoid systems and thus has the potential for a broad impact on basic and translational organoid studies.Developing therapies for patients with kidney diseases relies on a morphologically and physiologically representative in vitro model. Human kidney organoids are an attractive model to recapitulate kidney physiology, however, they are limited by the absence of a vascular network and mature cell populations. In this work, we have generated a genetically inducible endothelial niche that, when combined with an established kidney organoid protocol, induces the maturation of a robust endothelial cell network, induces a more mature podocyte population, and induces the emergence a functional renin population. This advance significantly increases the clinical relevance of human kidney organoids for etiological studies of kidney disease and future regenerative medicine strategies." @default.
• W4378833691 created "2023-06-01" @default.
• W4378833691 creator A5002572018 @default.
• W4378833691 creator A5005368488 @default.
• W4378833691 creator A5009054892 @default.
• W4378833691 creator A5021224208 @default.
• W4378833691 creator A5022190868 @default.
• W4378833691 creator A5024874881 @default.
• W4378833691 creator A5026506282 @default.
• W4378833691 creator A5028774736 @default.
• W4378833691 creator A5030279380 @default.
• W4378833691 creator A5033965456 @default.
• W4378833691 creator A5036851075 @default.
• W4378833691 creator A5038463777 @default.
• W4378833691 creator A5041504988 @default.
• W4378833691 creator A5048905909 @default.
• W4378833691 creator A5055129942 @default.
• W4378833691 creator A5056643989 @default.
• W4378833691 creator A5057198657 @default.
• W4378833691 creator A5061723702 @default.
• W4378833691 creator A5075625083 @default.
• W4378833691 creator A5077176663 @default.
• W4378833691 creator A5081866388 @default.
• W4378833691 creator A5087035206 @default.
• W4378833691 creator A5088924932 @default.
• W4378833691 creator A5089488228 @default.
• W4378833691 creator A5091518312 @default.
• W4378833691 date "2023-05-30" @default.
• W4378833691 modified "2023-09-30" @default.
• W4378833691 title "Genetically engineering endothelial niche in human kidney organoids enables multilineage maturation, vascularization and de novo cell types" @default.
• W4378833691 cites W1802574002 @default.
• W4378833691 cites W1965378998 @default.
• W4378833691 cites W1969338661 @default.
• W4378833691 cites W1984883254 @default.
• W4378833691 cites W1990051447 @default.
• W4378833691 cites W1995605291 @default.
• W4378833691 cites W1997508706 @default.
• W4378833691 cites W1998740840 @default.
• W4378833691 cites W2001952247 @default.
• W4378833691 cites W2006258383 @default.
• W4378833691 cites W2008834586 @default.
• W4378833691 cites W2018202875 @default.
• W4378833691 cites W2030414936 @default.
• W4378833691 cites W2038403939 @default.
• W4378833691 cites W2049016944 @default.
• W4378833691 cites W2053782858 @default.
• W4378833691 cites W2088066303 @default.
• W4378833691 cites W2089771697 @default.
• W4378833691 cites W2103017472 @default.
• W4378833691 cites W2122238986 @default.
• W4378833691 cites W2141563055 @default.
• W4378833691 cites W2151854909 @default.
• W4378833691 cites W2153534186 @default.
• W4378833691 cites W2154921775 @default.
• W4378833691 cites W2166423354 @default.
• W4378833691 cites W2168164971 @default.
• W4378833691 cites W2203529929 @default.
• W4378833691 cites W2239087504 @default.
• W4378833691 cites W2264879763 @default.
• W4378833691 cites W2293074557 @default.
• W4378833691 cites W2295454854 @default.
• W4378833691 cites W2345356016 @default.
• W4378833691 cites W2472574803 @default.
• W4378833691 cites W2481994947 @default.
• W4378833691 cites W2518654183 @default.
• W4378833691 cites W2581946541 @default.
• W4378833691 cites W2605541344 @default.
• W4378833691 cites W2616033863 @default.
• W4378833691 cites W2747877289 @default.
• W4378833691 cites W2787158099 @default.
• W4378833691 cites W2789166354 @default.
• W4378833691 cites W2791909710 @default.
• W4378833691 cites W2803499747 @default.
• W4378833691 cites W2884811309 @default.
• W4378833691 cites W2903061871 @default.
• W4378833691 cites W2904111838 @default.
• W4378833691 cites W2907412628 @default.
• W4378833691 cites W2908814343 @default.
• W4378833691 cites W2913348902 @default.
• W4378833691 cites W2915844676 @default.
• W4378833691 cites W2916020270 @default.
• W4378833691 cites W2945746490 @default.
• W4378833691 cites W2951506174 @default.
• W4378833691 cites W2955066631 @default.
• W4378833691 cites W2963202064 @default.
• W4378833691 cites W2964845115 @default.
• W4378833691 cites W2966569571 @default.
• W4378833691 cites W2968650613 @default.
• W4378833691 cites W2968997008 @default.
• W4378833691 cites W2971570665 @default.
• W4378833691 cites W2978986459 @default.
• W4378833691 cites W2984472267 @default.
• W4378833691 cites W2990251838 @default.
• W4378833691 cites W2995343362 @default.
• W4378833691 cites W2995769599 @default.
• W4378833691 cites W3027249627 @default.
• W4378833691 cites W3036487861 @default.
• W4378833691 cites W3037280785 @default.
• W4378833691 cites W3081483159 @default.
• W4378833691 cites W3083915852 @default.

• next