Matches in SemOpenAlex for { <https://semopenalex.org/work/W4378906905> ?p ?o ?g. }
- W4378906905 endingPage "1186" @default.
- W4378906905 startingPage "1186" @default.
- W4378906905 abstract "The development of treatment strategies for human corneal endothelial cells (hCECs) disease is necessary because hCECs do not regenerate in vivo due to the properties that are similar to senescence. This study is performed to investigate the role of a p-Tyr42 RhoA inhibitor (MH4, ELMED Inc., Chuncheon) in transforming growth factor-beta (TGF-β)- or H2O2-induced cellular senescence of hCECs. Cultured hCECs were treated with MH4. The cell shape, proliferation rate, and cell cycle phases were analyzed. Moreover, cell adhesion assays and immunofluorescence staining for F-actin, Ki-67, and E-cadherin were performed. Additionally, the cells were treated with TGF-β or H2O2 to induce senescence, and mitochondrial oxidative reactive oxygen species (ROS) levels, mitochondrial membrane potential, and NF-κB translocation were evaluated. LC3II/LC3I levels were determined using Western blotting to analyze autophagy. MH4 promotes hCEC proliferation, shifts the cell cycle, attenuates actin distribution, and increases E-cadherin expression. TGF-β and H2O2 induce senescence by increasing mitochondrial ROS levels and NF-κB translocation into the nucleus; however, this effect is attenuated by MH4. Moreover, TGF-β and H2O2 decrease the mitochondrial membrane potential and induce autophagy, while MH4 reverses these effects. In conclusion, MH4, a p-Tyr42 RhoA inhibitor, promotes the regeneration of hCECs and protects hCECs against TGF-β- and H2O2-induced senescence via the ROS/NF-κB/mitochondrial pathway." @default.
- W4378906905 created "2023-06-01" @default.
- W4378906905 creator A5006940360 @default.
- W4378906905 creator A5016609653 @default.
- W4378906905 creator A5019521932 @default.
- W4378906905 creator A5022432163 @default.
- W4378906905 creator A5058293048 @default.
- W4378906905 creator A5088950856 @default.
- W4378906905 date "2023-05-30" @default.
- W4378906905 modified "2023-10-14" @default.
- W4378906905 title "A p-Tyr42 RhoA Inhibitor Promotes the Regeneration of Human Corneal Endothelial Cells by Ameliorating Cellular Senescence" @default.
- W4378906905 cites W1581216242 @default.
- W4378906905 cites W1602153797 @default.
- W4378906905 cites W1915485606 @default.
- W4378906905 cites W1966405105 @default.
- W4378906905 cites W1971708920 @default.
- W4378906905 cites W1981885955 @default.
- W4378906905 cites W2001720505 @default.
- W4378906905 cites W2006474545 @default.
- W4378906905 cites W2008416190 @default.
- W4378906905 cites W2015439380 @default.
- W4378906905 cites W2025035335 @default.
- W4378906905 cites W2034691102 @default.
- W4378906905 cites W2042611486 @default.
- W4378906905 cites W2060903279 @default.
- W4378906905 cites W2066402175 @default.
- W4378906905 cites W2076598575 @default.
- W4378906905 cites W2080878133 @default.
- W4378906905 cites W2093147113 @default.
- W4378906905 cites W2101072720 @default.
- W4378906905 cites W2104005232 @default.
- W4378906905 cites W2116562304 @default.
- W4378906905 cites W2122308499 @default.
- W4378906905 cites W2124649657 @default.
- W4378906905 cites W2130429592 @default.
- W4378906905 cites W2131270640 @default.
- W4378906905 cites W2131271579 @default.
- W4378906905 cites W2131916409 @default.
- W4378906905 cites W2136289695 @default.
- W4378906905 cites W2145123926 @default.
- W4378906905 cites W2154903097 @default.
- W4378906905 cites W2166196909 @default.
- W4378906905 cites W2171365654 @default.
- W4378906905 cites W2178494498 @default.
- W4378906905 cites W2179438025 @default.
- W4378906905 cites W2188225742 @default.
- W4378906905 cites W2254996730 @default.
- W4378906905 cites W2272296579 @default.
- W4378906905 cites W2278220345 @default.
- W4378906905 cites W2303438750 @default.
- W4378906905 cites W2350256796 @default.
- W4378906905 cites W2460037848 @default.
- W4378906905 cites W2462505067 @default.
- W4378906905 cites W2520842278 @default.
- W4378906905 cites W2527890107 @default.
- W4378906905 cites W2567363845 @default.
- W4378906905 cites W2587679548 @default.
- W4378906905 cites W2607389896 @default.
- W4378906905 cites W2613019008 @default.
- W4378906905 cites W2734942203 @default.
- W4378906905 cites W2735590725 @default.
- W4378906905 cites W2737537149 @default.
- W4378906905 cites W2768129277 @default.
- W4378906905 cites W2791645463 @default.
- W4378906905 cites W2791975532 @default.
- W4378906905 cites W2874842801 @default.
- W4378906905 cites W2898257384 @default.
- W4378906905 cites W2900903241 @default.
- W4378906905 cites W2902207904 @default.
- W4378906905 cites W2903919207 @default.
- W4378906905 cites W2960482396 @default.
- W4378906905 cites W2963901310 @default.
- W4378906905 cites W2964849163 @default.
- W4378906905 cites W2965123080 @default.
- W4378906905 cites W2966094674 @default.
- W4378906905 cites W2971657453 @default.
- W4378906905 cites W2979971613 @default.
- W4378906905 cites W2997404986 @default.
- W4378906905 cites W3002644695 @default.
- W4378906905 cites W3004905553 @default.
- W4378906905 cites W3012177665 @default.
- W4378906905 cites W3014546563 @default.
- W4378906905 cites W3016130545 @default.
- W4378906905 cites W3023011061 @default.
- W4378906905 cites W3083152579 @default.
- W4378906905 cites W3087682214 @default.
- W4378906905 cites W3091390800 @default.
- W4378906905 cites W3115419007 @default.
- W4378906905 cites W3138266355 @default.
- W4378906905 cites W3138783510 @default.
- W4378906905 cites W3138872986 @default.
- W4378906905 cites W3167086420 @default.
- W4378906905 cites W3170855775 @default.
- W4378906905 cites W3190631166 @default.
- W4378906905 cites W3194706499 @default.
- W4378906905 cites W3199795725 @default.
- W4378906905 cites W3200996204 @default.
- W4378906905 cites W3210920306 @default.