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- W4379013318 abstract "The rise in consumption of ultra-processed meat and dietary collagen supplements challenges food quality control. 4-hydroxyproline stands out as collagen signature amino acid, and its determination is a well-known way to estimate collagen content, although for this measurement, the majority of the methods require a mandatory derivatization step. Therefore, this study aimed to develop an innovative method using cyclodextrin-assisted electrokinetic chromatography with indirect UV detection to determine 4-hydroxyproline for collagen monitoring in dietary supplements and meat products. Separations were performed in fused silica capillaries, at 12.5 °C, detection at 220 nm, 30 kV voltage, and hydrodynamic injection. Background electrolyte consisted of histidine 25 mmol L-1, butylamine 180 mmol L-1, and β-cyclodextrin 10 mmol L-1 at pH 11.4. Samples were hydrolyzed with sulfuric acid, and their removal was accomplished by simultaneous neutralization/precipitation reactions using strontium carbonate. Validation assured method detectability, accuracy (recoveries 88% - 102%), and precision (CV < 7%). Dietary supplements and meat products were successfully evaluated, indicating that this method is a feasible quality control alternative." @default.
- W4379013318 created "2023-06-02" @default.
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- W4379013318 date "2023-09-01" @default.
- W4379013318 modified "2023-10-16" @default.
- W4379013318 title "Development of a new method for the determination of 4-hydroxyproline as a measurement of collagen content in meat products and dietary supplements by cyclodextrin-assisted electrokinetic chromatography" @default.
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- W4379013318 doi "https://doi.org/10.1016/j.jfca.2023.105431" @default.
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