FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4379054193> ?p ?o ?g. }

• W4379054193 endingPage "2189" @default.
• W4379054193 startingPage "2189" @default.
• W4379054193 abstract "With increasing throughput in both the generation and phenotyping of mutant lines in plants, it is important to have an efficient and reliable genotyping method. Traditional workflows, still commonly used in many labs, have time-consuming and expensive steps, such as DNA purification, cloning and growing E. coli cultures. We propose an alternative workflow where these steps are bypassed, using Phire polymerase on fresh plant tissue, and ExoProStar treatment as preparation for sequencing. We generated CRISPR-Cas9 mutants for ZAS (ZAXINONE SYNTHASE) in rice with two guide RNAs. Using both a traditional workflow and our proposed workflow, we genotyped nine T1 plants. To interpret the sequencing output, which is often complex in CRISPR-generated mutants, we used free online automatic analysis systems and compared the results. Our proposed workflow produces results of the same quality as the old workflow, but in 1 day instead of 3 days and about 35 times cheaper. This workflow also consists of fewer steps and reduces the risk of cross contamination and mistakes. Furthermore, the automated sequence analysis packages are mostly accurate and could easily be used for bulk analysis. Based on these advantages, we encourage academic and commercial labs conducting genotyping to consider switching over to our proposed workflow." @default.
• W4379054193 created "2023-06-02" @default.
• W4379054193 creator A5021985442 @default.
• W4379054193 creator A5033800162 @default.
• W4379054193 creator A5058587504 @default.
• W4379054193 creator A5059193364 @default.
• W4379054193 creator A5067805586 @default.
• W4379054193 date "2023-05-31" @default.
• W4379054193 modified "2023-09-26" @default.
• W4379054193 title "A Fast and Cost-Effective Genotyping Method for CRISPR-Cas9-Generated Mutant Rice Lines" @default.
• W4379054193 cites W1940605149 @default.
• W4379054193 cites W1988768289 @default.
• W4379054193 cites W1992783297 @default.
• W4379054193 cites W2001845502 @default.
• W4379054193 cites W2006807779 @default.
• W4379054193 cites W2010851273 @default.
• W4379054193 cites W2045435533 @default.
• W4379054193 cites W2049741581 @default.
• W4379054193 cites W2057847071 @default.
• W4379054193 cites W2059661893 @default.
• W4379054193 cites W2076583629 @default.
• W4379054193 cites W2111990626 @default.
• W4379054193 cites W2129246217 @default.
• W4379054193 cites W2145878592 @default.
• W4379054193 cites W2173737475 @default.
• W4379054193 cites W2344454439 @default.
• W4379054193 cites W2460640366 @default.
• W4379054193 cites W2588178612 @default.
• W4379054193 cites W2593080775 @default.
• W4379054193 cites W2743099781 @default.
• W4379054193 cites W2888868862 @default.
• W4379054193 cites W2914588700 @default.
• W4379054193 cites W2917778527 @default.
• W4379054193 cites W2950704869 @default.
• W4379054193 cites W2972628085 @default.
• W4379054193 cites W3005209155 @default.
• W4379054193 cites W3025096875 @default.
• W4379054193 cites W3127729088 @default.
• W4379054193 cites W3209658415 @default.
• W4379054193 cites W4210762403 @default.
• W4379054193 cites W4287578435 @default.
• W4379054193 cites W4304731472 @default.
• W4379054193 cites W4306292872 @default.
• W4379054193 doi "https://doi.org/10.3390/plants12112189" @default.
• W4379054193 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37299168" @default.
• W4379054193 hasPublicationYear "2023" @default.
• W4379054193 type Work @default.
• W4379054193 citedByCount "0" @default.
• W4379054193 crossrefType "journal-article" @default.
• W4379054193 hasAuthorship W4379054193A5021985442 @default.
• W4379054193 hasAuthorship W4379054193A5033800162 @default.
• W4379054193 hasAuthorship W4379054193A5058587504 @default.
• W4379054193 hasAuthorship W4379054193A5059193364 @default.
• W4379054193 hasAuthorship W4379054193A5067805586 @default.
• W4379054193 hasBestOaLocation W43790541931 @default.
• W4379054193 hasConcept C104317684 @default.
• W4379054193 hasConcept C111919701 @default.
• W4379054193 hasConcept C121050878 @default.
• W4379054193 hasConcept C132455925 @default.
• W4379054193 hasConcept C135763542 @default.
• W4379054193 hasConcept C143065580 @default.
• W4379054193 hasConcept C157764524 @default.
• W4379054193 hasConcept C177212765 @default.
• W4379054193 hasConcept C199360897 @default.
• W4379054193 hasConcept C31467283 @default.
• W4379054193 hasConcept C41008148 @default.
• W4379054193 hasConcept C51679486 @default.
• W4379054193 hasConcept C54355233 @default.
• W4379054193 hasConcept C552990157 @default.
• W4379054193 hasConcept C555944384 @default.
• W4379054193 hasConcept C70721500 @default.
• W4379054193 hasConcept C77088390 @default.
• W4379054193 hasConcept C86803240 @default.
• W4379054193 hasConcept C98108389 @default.
• W4379054193 hasConceptScore W4379054193C104317684 @default.
• W4379054193 hasConceptScore W4379054193C111919701 @default.
• W4379054193 hasConceptScore W4379054193C121050878 @default.
• W4379054193 hasConceptScore W4379054193C132455925 @default.
• W4379054193 hasConceptScore W4379054193C135763542 @default.
• W4379054193 hasConceptScore W4379054193C143065580 @default.
• W4379054193 hasConceptScore W4379054193C157764524 @default.
• W4379054193 hasConceptScore W4379054193C177212765 @default.
• W4379054193 hasConceptScore W4379054193C199360897 @default.
• W4379054193 hasConceptScore W4379054193C31467283 @default.
• W4379054193 hasConceptScore W4379054193C41008148 @default.
• W4379054193 hasConceptScore W4379054193C51679486 @default.
• W4379054193 hasConceptScore W4379054193C54355233 @default.
• W4379054193 hasConceptScore W4379054193C552990157 @default.
• W4379054193 hasConceptScore W4379054193C555944384 @default.
• W4379054193 hasConceptScore W4379054193C70721500 @default.
• W4379054193 hasConceptScore W4379054193C77088390 @default.
• W4379054193 hasConceptScore W4379054193C86803240 @default.
• W4379054193 hasConceptScore W4379054193C98108389 @default.
• W4379054193 hasFunder F4320322320 @default.
• W4379054193 hasIssue "11" @default.
• W4379054193 hasLocation W43790541931 @default.
• W4379054193 hasLocation W43790541932 @default.
• W4379054193 hasOpenAccess W4379054193 @default.

• next