Matches in SemOpenAlex for { <https://semopenalex.org/work/W4379233530> ?p ?o ?g. }
- W4379233530 endingPage "746" @default.
- W4379233530 startingPage "734" @default.
- W4379233530 abstract "Enzymatic processes are industrially important. Process duration, temperature, and pH are critical factors to optimize. Longer processes can improve economics by requiring less enzyme. Both temperature and pH affect enzymes, but temperature has far stronger interactive effects with process duration, which has scarcely been considered. To fill this technology gap, we investigated both short- and long-term temperature effects on Aspergillus niger carbohydrases. For short-term effects, enzyme activities were measured at different temperatures and regressed with a model that considered catalytic activation-energy and protein-folding. The model-determined short-term optima were: α-galactosidase, 57.6 °C; sucrase, 53.4 °C; pectinase, 49.4 °C; xylanase, 50.4 °C; and cellulase, 46.5 °C. Long-term effects were evaluated at 40, 52, 55, 60, and 65 °C for 72 h to determine the activity decay constants and activation energies. The observed order of stability was sucrase > α-galactosidase > pectinase > xylanase, while cellulase showed more complex decay kinetics. These models enabled prediction of cumulative enzymatic performance at different temperatures and durations. The prediction for α-galactosidase was verified with experimental results of soybean molasses hydrolysis, giving 51% higher stachyose conversion after 72 h at 54 °C (predicted optimum) than 60 °C. The models developed and approach taken are valuable for optimizing enzymatic processes, which improve sustainability and environmental friendliness." @default.
- W4379233530 created "2023-06-04" @default.
- W4379233530 creator A5024803902 @default.
- W4379233530 creator A5028544647 @default.
- W4379233530 date "2023-07-01" @default.
- W4379233530 modified "2023-10-16" @default.
- W4379233530 title "On optimization of enzymatic processes: Temperature effects on activity and long-term deactivation kinetics" @default.
- W4379233530 cites W1507625409 @default.
- W4379233530 cites W1969056401 @default.
- W4379233530 cites W1975222947 @default.
- W4379233530 cites W1995588635 @default.
- W4379233530 cites W1998752513 @default.
- W4379233530 cites W1999736100 @default.
- W4379233530 cites W2002576908 @default.
- W4379233530 cites W2005015667 @default.
- W4379233530 cites W2011224222 @default.
- W4379233530 cites W2019200192 @default.
- W4379233530 cites W2029378851 @default.
- W4379233530 cites W2043263745 @default.
- W4379233530 cites W2054972004 @default.
- W4379233530 cites W2055712220 @default.
- W4379233530 cites W2059563894 @default.
- W4379233530 cites W2077854669 @default.
- W4379233530 cites W2079257291 @default.
- W4379233530 cites W2090353177 @default.
- W4379233530 cites W2095121456 @default.
- W4379233530 cites W2101943401 @default.
- W4379233530 cites W2102144840 @default.
- W4379233530 cites W2107268560 @default.
- W4379233530 cites W2112723141 @default.
- W4379233530 cites W2126030240 @default.
- W4379233530 cites W2127876682 @default.
- W4379233530 cites W2133691858 @default.
- W4379233530 cites W2136162596 @default.
- W4379233530 cites W2148041910 @default.
- W4379233530 cites W2148541186 @default.
- W4379233530 cites W2258180218 @default.
- W4379233530 cites W2325757558 @default.
- W4379233530 cites W2333446718 @default.
- W4379233530 cites W2507123105 @default.
- W4379233530 cites W2569258853 @default.
- W4379233530 cites W2603998914 @default.
- W4379233530 cites W2606827187 @default.
- W4379233530 cites W2805241968 @default.
- W4379233530 cites W2910355691 @default.
- W4379233530 cites W2952731827 @default.
- W4379233530 cites W2980702156 @default.
- W4379233530 cites W2982874370 @default.
- W4379233530 cites W2983473168 @default.
- W4379233530 cites W3001466143 @default.
- W4379233530 cites W3022335475 @default.
- W4379233530 cites W3032584551 @default.
- W4379233530 cites W3035129734 @default.
- W4379233530 cites W3035847987 @default.
- W4379233530 cites W3036681005 @default.
- W4379233530 cites W3043794783 @default.
- W4379233530 cites W3215719781 @default.
- W4379233530 cites W4205544451 @default.
- W4379233530 doi "https://doi.org/10.1016/j.procbio.2023.05.031" @default.
- W4379233530 hasPublicationYear "2023" @default.
- W4379233530 type Work @default.
- W4379233530 citedByCount "0" @default.
- W4379233530 crossrefType "journal-article" @default.
- W4379233530 hasAuthorship W4379233530A5024803902 @default.
- W4379233530 hasAuthorship W4379233530A5028544647 @default.
- W4379233530 hasBestOaLocation W43792335301 @default.
- W4379233530 hasConcept C121332964 @default.
- W4379233530 hasConcept C141603559 @default.
- W4379233530 hasConcept C148898269 @default.
- W4379233530 hasConcept C172854840 @default.
- W4379233530 hasConcept C181199279 @default.
- W4379233530 hasConcept C185592680 @default.
- W4379233530 hasConcept C2775859485 @default.
- W4379233530 hasConcept C2776039999 @default.
- W4379233530 hasConcept C55493867 @default.
- W4379233530 hasConcept C62520636 @default.
- W4379233530 hasConcept C97355855 @default.
- W4379233530 hasConceptScore W4379233530C121332964 @default.
- W4379233530 hasConceptScore W4379233530C141603559 @default.
- W4379233530 hasConceptScore W4379233530C148898269 @default.
- W4379233530 hasConceptScore W4379233530C172854840 @default.
- W4379233530 hasConceptScore W4379233530C181199279 @default.
- W4379233530 hasConceptScore W4379233530C185592680 @default.
- W4379233530 hasConceptScore W4379233530C2775859485 @default.
- W4379233530 hasConceptScore W4379233530C2776039999 @default.
- W4379233530 hasConceptScore W4379233530C55493867 @default.
- W4379233530 hasConceptScore W4379233530C62520636 @default.
- W4379233530 hasConceptScore W4379233530C97355855 @default.
- W4379233530 hasFunder F4320313191 @default.
- W4379233530 hasLocation W43792335301 @default.
- W4379233530 hasOpenAccess W4379233530 @default.
- W4379233530 hasPrimaryLocation W43792335301 @default.
- W4379233530 hasRelatedWork W1835129738 @default.
- W4379233530 hasRelatedWork W1965651000 @default.
- W4379233530 hasRelatedWork W1967849171 @default.
- W4379233530 hasRelatedWork W2072542022 @default.
- W4379233530 hasRelatedWork W2150694461 @default.
- W4379233530 hasRelatedWork W2508534631 @default.