Matches in SemOpenAlex for { <https://semopenalex.org/work/W4379259715> ?p ?o ?g. }
- W4379259715 abstract "The influence of nanoparticles inside the human body and their interactions with biological macromolecules need to be explored/studied prior to specific applications. The objective of this study is to find the potential of camptothecin functionalised silver nanoparticles (CMT-AgNPs) in biomedical applications. This article primarily investigates the binding stratagem of CMT-AgNPs with calf thymus DNA (ctDNA) through a series of spectroscopic and calorimetric methods and then analyses the anticancer activity and cytotoxicity of CMT-AgNPs. The nanoparticles were synthesized using a simple one pot method and characterized using UV-Visible, fourier transform infrared (FTIR) spectroscopy, X-ray diffraction and high-resolution transmission electron microscopy (HRTEM). The average size of CMT-AgNPs is 10 ± 2 nm. A group of experimental techniques such as UV-Visible spectrophotometry, fluorescence dye displacement assay, circular dichroism (CD) and viscosity analysis unravelled the typical groove binding mode of CMT-AgNPs with ctDNA. The CD measurement evidenced the minor conformational alterations of double helical structure of ctDNA in the presence of CMT-AgNPs. The information deduced from the isothermal titration calorimetry (ITC) experiment is that the binding was exothermic and spontaneous in nature. Moreover, all the thermodynamic binding parameters were extracted from the ITC data. The binding constants obtained from UV absorption experiments, fluorescence dye displacement studies and ITC were consistently in the order of 104 Mol-1. All these results validated the formation of CMT-AgNPs-ctDNA complex and the results unambiguously confirm the typical groove binding mode of CMT-AgNPs. An exhaustive in vitro MTT assay by CMT-AgNPs and CMT against A549, HT29, HeLa and L929 cell lines revealed the capability of CMT-AgNPs as a potential anticancer agent." @default.
- W4379259715 created "2023-06-04" @default.
- W4379259715 creator A5002606004 @default.
- W4379259715 creator A5018109250 @default.
- W4379259715 creator A5053213306 @default.
- W4379259715 creator A5058457530 @default.
- W4379259715 creator A5074667570 @default.
- W4379259715 creator A5078281964 @default.
- W4379259715 date "2023-06-03" @default.
- W4379259715 modified "2023-10-01" @default.
- W4379259715 title "Exploring the cytotoxicity on human lung cancer cells and DNA binding stratagem of camptothecin functionalised silver nanoparticles through multi-spectroscopic, and calorimetric approach" @default.
- W4379259715 cites W1543790510 @default.
- W4379259715 cites W1950590558 @default.
- W4379259715 cites W1965272410 @default.
- W4379259715 cites W1972472207 @default.
- W4379259715 cites W1990038882 @default.
- W4379259715 cites W2000000511 @default.
- W4379259715 cites W2008757142 @default.
- W4379259715 cites W2011321566 @default.
- W4379259715 cites W2012589943 @default.
- W4379259715 cites W2039658296 @default.
- W4379259715 cites W2060001961 @default.
- W4379259715 cites W2061137761 @default.
- W4379259715 cites W2061925251 @default.
- W4379259715 cites W2064065404 @default.
- W4379259715 cites W2064583588 @default.
- W4379259715 cites W2066411725 @default.
- W4379259715 cites W2067118833 @default.
- W4379259715 cites W2070534090 @default.
- W4379259715 cites W2071228760 @default.
- W4379259715 cites W2086325556 @default.
- W4379259715 cites W2086353181 @default.
- W4379259715 cites W2087649671 @default.
- W4379259715 cites W2090745860 @default.
- W4379259715 cites W2124202484 @default.
- W4379259715 cites W2134789227 @default.
- W4379259715 cites W2148767282 @default.
- W4379259715 cites W2160048770 @default.
- W4379259715 cites W2201819712 @default.
- W4379259715 cites W2269389638 @default.
- W4379259715 cites W2284055764 @default.
- W4379259715 cites W2301677130 @default.
- W4379259715 cites W2331319068 @default.
- W4379259715 cites W2395464042 @default.
- W4379259715 cites W2434866026 @default.
- W4379259715 cites W2518046346 @default.
- W4379259715 cites W2556513287 @default.
- W4379259715 cites W2560223743 @default.
- W4379259715 cites W2593167667 @default.
- W4379259715 cites W2596896008 @default.
- W4379259715 cites W2624854612 @default.
- W4379259715 cites W2679767274 @default.
- W4379259715 cites W2742322428 @default.
- W4379259715 cites W2745559177 @default.
- W4379259715 cites W2782450689 @default.
- W4379259715 cites W2789307612 @default.
- W4379259715 cites W2790859638 @default.
- W4379259715 cites W2794128681 @default.
- W4379259715 cites W2892178840 @default.
- W4379259715 cites W2905756335 @default.
- W4379259715 cites W2925471850 @default.
- W4379259715 cites W2944115988 @default.
- W4379259715 cites W2957833068 @default.
- W4379259715 cites W2972586133 @default.
- W4379259715 cites W2973273159 @default.
- W4379259715 cites W2979527418 @default.
- W4379259715 cites W2994686318 @default.
- W4379259715 cites W2995708286 @default.
- W4379259715 cites W3005689864 @default.
- W4379259715 cites W3009630077 @default.
- W4379259715 cites W3012611578 @default.
- W4379259715 cites W3035320234 @default.
- W4379259715 cites W3080202315 @default.
- W4379259715 cites W3088482453 @default.
- W4379259715 cites W3091905208 @default.
- W4379259715 cites W3097054898 @default.
- W4379259715 cites W3112666381 @default.
- W4379259715 cites W3142197916 @default.
- W4379259715 cites W3157639946 @default.
- W4379259715 cites W3164314196 @default.
- W4379259715 cites W4205165914 @default.
- W4379259715 cites W4281486506 @default.
- W4379259715 cites W4283735066 @default.
- W4379259715 cites W4286250188 @default.
- W4379259715 cites W4293576628 @default.
- W4379259715 cites W4307551736 @default.
- W4379259715 cites W4312224851 @default.
- W4379259715 cites W4313559465 @default.
- W4379259715 cites W4317935541 @default.
- W4379259715 cites W4318940240 @default.
- W4379259715 cites W4319304764 @default.
- W4379259715 cites W4320176220 @default.
- W4379259715 cites W4320500297 @default.
- W4379259715 cites W4322495823 @default.
- W4379259715 cites W4322719310 @default.
- W4379259715 cites W4323315308 @default.
- W4379259715 cites W634160248 @default.
- W4379259715 doi "https://doi.org/10.1038/s41598-023-34997-w" @default.
- W4379259715 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37270606" @default.
- W4379259715 hasPublicationYear "2023" @default.