Matches in SemOpenAlex for { <https://semopenalex.org/work/W4379515712> ?p ?o ?g. }
- W4379515712 abstract "ABSTRACT Sustainably enhancing crop production is a necessity given the increasing demands for staple crops and their associated carbon/nitrogen inputs. Plant-associated microbiomes offer one avenue for addressing this demand; however, studying these communities and engineering them has remained a challenge due to limited genetic tools and methods. In this work, we detail the development of the Maize Root ToolKit (MRTK); a rapid Modular Cloning (MoClo) toolkit that only takes 2.5 hours to generate desired constructs (5400 potential plasmids) that replicate and express heterologous genes in Enterobacter ludwigii strain AA4 ( Elu ), Pseudomonas putida AA7 ( Ppu ), Herbaspirillum robiniae strain AA6 ( Hro ), Stenotrophomonas maltophilia strain AA1 ( Sma ) and Brucella pituitosa strain AA2 ( Bpi ) which comprise a model maize root synthetic community (SynCom). In addition to these genetic tools, we describe a highly efficient transformation protocol (10^7-10^9 transformants/µg of DNA) for each of these strains. Utilizing this highly efficient transformation protocol, we identified endogenous expression sequences for each strain (ES; promoter and ribosomal binding sites) via genomic promoter trapping. Overall, the MRTK is a scalable platform that expands the genetic engineering toolbox while providing a standardized, high efficiency transformation method that can be implemented across a diverse group of root commensals. These results unlock the ability to elucidate and engineer plant-microbe interactions promoting plant growth for each of the 5 bacterial strains in this study." @default.
- W4379515712 created "2023-06-07" @default.
- W4379515712 creator A5007446672 @default.
- W4379515712 creator A5033994793 @default.
- W4379515712 creator A5058374954 @default.
- W4379515712 creator A5074528419 @default.
- W4379515712 date "2023-06-05" @default.
- W4379515712 modified "2023-09-25" @default.
- W4379515712 title "Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and Identification of Potential Bacterial Chassis for studying Plant-Microbe Interactions" @default.
- W4379515712 cites W1769506315 @default.
- W4379515712 cites W1782558822 @default.
- W4379515712 cites W1966329468 @default.
- W4379515712 cites W1968793608 @default.
- W4379515712 cites W1976303268 @default.
- W4379515712 cites W2003766560 @default.
- W4379515712 cites W2006085890 @default.
- W4379515712 cites W2008199833 @default.
- W4379515712 cites W2017364432 @default.
- W4379515712 cites W2019075610 @default.
- W4379515712 cites W2045476884 @default.
- W4379515712 cites W2048628486 @default.
- W4379515712 cites W2061104342 @default.
- W4379515712 cites W2085558835 @default.
- W4379515712 cites W2101442604 @default.
- W4379515712 cites W2120263648 @default.
- W4379515712 cites W2124800145 @default.
- W4379515712 cites W2128988512 @default.
- W4379515712 cites W2142388360 @default.
- W4379515712 cites W2158787765 @default.
- W4379515712 cites W2171012458 @default.
- W4379515712 cites W2252306275 @default.
- W4379515712 cites W2327010950 @default.
- W4379515712 cites W2336950589 @default.
- W4379515712 cites W2340320312 @default.
- W4379515712 cites W2414991245 @default.
- W4379515712 cites W2592495682 @default.
- W4379515712 cites W2598850214 @default.
- W4379515712 cites W2737972386 @default.
- W4379515712 cites W2741747812 @default.
- W4379515712 cites W2744640382 @default.
- W4379515712 cites W2766189659 @default.
- W4379515712 cites W2766283429 @default.
- W4379515712 cites W2781978265 @default.
- W4379515712 cites W2784680706 @default.
- W4379515712 cites W2889507071 @default.
- W4379515712 cites W2897706611 @default.
- W4379515712 cites W2903579321 @default.
- W4379515712 cites W2921198002 @default.
- W4379515712 cites W2925474198 @default.
- W4379515712 cites W2943667167 @default.
- W4379515712 cites W2944902712 @default.
- W4379515712 cites W2947225886 @default.
- W4379515712 cites W2947939038 @default.
- W4379515712 cites W2951381508 @default.
- W4379515712 cites W2993316433 @default.
- W4379515712 cites W3048805481 @default.
- W4379515712 cites W3080458156 @default.
- W4379515712 cites W3138273830 @default.
- W4379515712 cites W3163366850 @default.
- W4379515712 cites W3169128895 @default.
- W4379515712 cites W3173984248 @default.
- W4379515712 cites W3184691306 @default.
- W4379515712 cites W3210655202 @default.
- W4379515712 cites W3216875165 @default.
- W4379515712 cites W3217457192 @default.
- W4379515712 cites W4205369256 @default.
- W4379515712 cites W4220710264 @default.
- W4379515712 cites W4229007032 @default.
- W4379515712 cites W4287832999 @default.
- W4379515712 cites W4294488523 @default.
- W4379515712 cites W4307815729 @default.
- W4379515712 cites W4309908918 @default.
- W4379515712 cites W4353108918 @default.
- W4379515712 doi "https://doi.org/10.1101/2023.06.05.543168" @default.
- W4379515712 hasPublicationYear "2023" @default.
- W4379515712 type Work @default.
- W4379515712 citedByCount "0" @default.
- W4379515712 crossrefType "posted-content" @default.
- W4379515712 hasAuthorship W4379515712A5007446672 @default.
- W4379515712 hasAuthorship W4379515712A5033994793 @default.
- W4379515712 hasAuthorship W4379515712A5058374954 @default.
- W4379515712 hasAuthorship W4379515712A5074528419 @default.
- W4379515712 hasBestOaLocation W43795157121 @default.
- W4379515712 hasConcept C104317684 @default.
- W4379515712 hasConcept C105702510 @default.
- W4379515712 hasConcept C143121216 @default.
- W4379515712 hasConcept C150903083 @default.
- W4379515712 hasConcept C204241405 @default.
- W4379515712 hasConcept C22744801 @default.
- W4379515712 hasConcept C2778022156 @default.
- W4379515712 hasConcept C2780543182 @default.
- W4379515712 hasConcept C54355233 @default.
- W4379515712 hasConcept C70721500 @default.
- W4379515712 hasConcept C86803240 @default.
- W4379515712 hasConceptScore W4379515712C104317684 @default.
- W4379515712 hasConceptScore W4379515712C105702510 @default.
- W4379515712 hasConceptScore W4379515712C143121216 @default.
- W4379515712 hasConceptScore W4379515712C150903083 @default.
- W4379515712 hasConceptScore W4379515712C204241405 @default.
- W4379515712 hasConceptScore W4379515712C22744801 @default.