Matches in SemOpenAlex for { <https://semopenalex.org/work/W4379523015> ?p ?o ?g. }
- W4379523015 abstract "Abstract The Multidrug Resistance Protein 1 (Mrp1) is an ATP-dependent efflux transporter and a major facilitator of drug resistance in mammalian cells during cancer and HIV therapy. In brain, Mrp1-mediated GSH export from astrocytes is the first step in the supply of GSH precursors to neurons. To reveal potential mechanisms underlying the drug-induced modulation of Mrp1-mediated transport processes, we investigated the effects of the antiviral drug ritonavir on cultured rat primary astrocytes. Ritonavir strongly stimulated the Mrp1-mediated export of glutathione (GSH) by decreasing the K m value from 200 nmol/mg to 28 nmol/mg. In contrast, ritonavir decreased the export of the other Mrp1 substrates glutathione disulfide (GSSG) and glutathione-bimane. To give explanation for these apparently contradictory observations, we performed in silico docking analysis and molecular dynamics simulations using a homology model of rat Mrp1 to predict the binding modes of ritonavir, GSH and GSSG to Mrp1. The results suggest that ritonavir binds to the hydrophilic part of the bipartite binding site of Mrp1 and thereby differently affects the binding and transport of the Mrp1 substrates. These new insights into the modulation of Mrp1-mediated export processes by ritonavir provide a new model to better understand GSH-dependent detoxification processes in brain cells." @default.
- W4379523015 created "2023-06-07" @default.
- W4379523015 creator A5003834545 @default.
- W4379523015 creator A5029370722 @default.
- W4379523015 creator A5034922405 @default.
- W4379523015 creator A5074413996 @default.
- W4379523015 creator A5077770397 @default.
- W4379523015 date "2023-06-06" @default.
- W4379523015 modified "2023-09-27" @default.
- W4379523015 title "Modulation of Multidrug Resistance Protein 1-mediated transport processes by the antiviral drug ritonavir in cultured primary astrocytes" @default.
- W4379523015 cites W1031578623 @default.
- W4379523015 cites W1479961581 @default.
- W4379523015 cites W1509452365 @default.
- W4379523015 cites W1519205980 @default.
- W4379523015 cites W1547339950 @default.
- W4379523015 cites W1558564171 @default.
- W4379523015 cites W1581924471 @default.
- W4379523015 cites W1775749144 @default.
- W4379523015 cites W1797316917 @default.
- W4379523015 cites W1970658820 @default.
- W4379523015 cites W1976499671 @default.
- W4379523015 cites W1981820162 @default.
- W4379523015 cites W1983094838 @default.
- W4379523015 cites W1985948217 @default.
- W4379523015 cites W1986986500 @default.
- W4379523015 cites W1987134040 @default.
- W4379523015 cites W1990771438 @default.
- W4379523015 cites W2003598937 @default.
- W4379523015 cites W2007588811 @default.
- W4379523015 cites W2019193021 @default.
- W4379523015 cites W2021520922 @default.
- W4379523015 cites W2022260179 @default.
- W4379523015 cites W2034611990 @default.
- W4379523015 cites W2035095394 @default.
- W4379523015 cites W2035266068 @default.
- W4379523015 cites W2035485154 @default.
- W4379523015 cites W2037312364 @default.
- W4379523015 cites W2040601222 @default.
- W4379523015 cites W2043494673 @default.
- W4379523015 cites W2064388657 @default.
- W4379523015 cites W2066414494 @default.
- W4379523015 cites W2067484084 @default.
- W4379523015 cites W2067851857 @default.
- W4379523015 cites W2069486511 @default.
- W4379523015 cites W2071927278 @default.
- W4379523015 cites W2071998110 @default.
- W4379523015 cites W2073587293 @default.
- W4379523015 cites W2092356823 @default.
- W4379523015 cites W2094458603 @default.
- W4379523015 cites W2097317964 @default.
- W4379523015 cites W2105668062 @default.
- W4379523015 cites W2109189857 @default.
- W4379523015 cites W2110790042 @default.
- W4379523015 cites W2114551134 @default.
- W4379523015 cites W2115985519 @default.
- W4379523015 cites W2119579918 @default.
- W4379523015 cites W2120635383 @default.
- W4379523015 cites W2125696348 @default.
- W4379523015 cites W2127157208 @default.
- W4379523015 cites W2128572087 @default.
- W4379523015 cites W2132357129 @default.
- W4379523015 cites W2143373379 @default.
- W4379523015 cites W2146411229 @default.
- W4379523015 cites W2148710732 @default.
- W4379523015 cites W2158534713 @default.
- W4379523015 cites W2159854260 @default.
- W4379523015 cites W2168070336 @default.
- W4379523015 cites W2169714384 @default.
- W4379523015 cites W2170711116 @default.
- W4379523015 cites W2237689958 @default.
- W4379523015 cites W2467247336 @default.
- W4379523015 cites W2486282557 @default.
- W4379523015 cites W2555870966 @default.
- W4379523015 cites W2586662902 @default.
- W4379523015 cites W2589923912 @default.
- W4379523015 cites W2609408991 @default.
- W4379523015 cites W2612598385 @default.
- W4379523015 cites W2619720640 @default.
- W4379523015 cites W2741957510 @default.
- W4379523015 cites W2765155499 @default.
- W4379523015 cites W2777467795 @default.
- W4379523015 cites W2781325586 @default.
- W4379523015 cites W2792062240 @default.
- W4379523015 cites W2803908182 @default.
- W4379523015 cites W2806763958 @default.
- W4379523015 cites W2884127226 @default.
- W4379523015 cites W2892373116 @default.
- W4379523015 cites W2901627745 @default.
- W4379523015 cites W2909644057 @default.
- W4379523015 cites W2910658653 @default.
- W4379523015 cites W2915580414 @default.
- W4379523015 cites W2945182885 @default.
- W4379523015 cites W3026032990 @default.
- W4379523015 cites W3028112641 @default.
- W4379523015 cites W3130681257 @default.
- W4379523015 cites W3159480848 @default.
- W4379523015 cites W3161058384 @default.
- W4379523015 cites W3173197060 @default.
- W4379523015 cites W4194355 @default.
- W4379523015 cites W4205217892 @default.