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- W4379538142 abstract "After extractive alkylation combined with plasma deproteinization, we used gas chromatography to assay for short-chain carboxylic acids from formic acid to valeric acid in plasma and urine. It was possible to provide highly sensitive analysis with 0.1-3.4 µg/mL as the limit of detection for plasma and 0.6-8.0 µg/mL for urine, with a correlation coefficient of 1.000 for the linear regression calibration curves. For plasma, deproteinization using ultrafiltration before extractive alkylation resulted in a higher sensitivity for acetic, propionic, butyric, and valeric acids compared with the method without deproteinization. The concentrations of formic acid and acetic acid were determined to be 6 µg/mL and 10 µg/mL, respectively, in the tested plasma, and 22 µg/mL and 32 µg/mL, respectively, in the tested urine. Concentrations from propionic acid to valeric acid were ≤ 1.3 µg/mL. In addition, high concentrations of sulfate, phosphate, hydrogen carbonate, ammonium, and/or sodium ions did not remarkably inhibit the derivatization of carboxylic acids, although hydrogen carbonate ions significantly inhibited that of formic acid." @default.
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- W4379538142 date "2023-06-06" @default.
- W4379538142 modified "2023-09-25" @default.
- W4379538142 title "Assay of short-chain carboxylic acids in plasma and urine using gas chromatography after extractive alkylation" @default.
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- W4379538142 doi "https://doi.org/10.1007/s44211-023-00361-1" @default.
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