Matches in SemOpenAlex for { <https://semopenalex.org/work/W4379797841> ?p ?o ?g. }
- W4379797841 abstract "Abstract Rieske non‐heme iron oxygenases (ROs) are primarily known for their ability to catalyze the stereoselective formation of vicinal cis ‐diols in a single step, endowing valuable products for pharmaceutical and chemical applications. In addition, ROs can catalyze several other oxidation reactions with high regio‐ and stereoselectivity and typically broad substrate scope. Owing to their dependence on multicomponent electron transfer, the majority of synthetic applications of ROs relies on recombinant whole‐cell catalysts. In this context, important properties of the multicomponent system that determine the catalytic efficiency, including electron transfer via redox partner proteins, stability and uncoupling, have been investigated to a lesser extent in recent years. Here, we show for one of the most prominent ROs, the cumene dioxygenase from Pseudomonas fluorescens IP01 (CDO) that by developing and optimizing an efficient in vitro system, high catalytic activities can be achieved. In addition, we highlight that an efficient and continuous supplementation of electrons to the oxygenase is required to sustain their catalytic activity, while uncoupling can be a major limitation in CDO efficiency and stability." @default.
- W4379797841 created "2023-06-09" @default.
- W4379797841 creator A5022981478 @default.
- W4379797841 creator A5026188070 @default.
- W4379797841 creator A5030580114 @default.
- W4379797841 creator A5092113149 @default.
- W4379797841 date "2023-07-20" @default.
- W4379797841 modified "2023-09-26" @default.
- W4379797841 title "An Optimized System for the Study of Rieske Oxygenase‐catalyzed Hydroxylation Reactions <i>In vitro</i>" @default.
- W4379797841 cites W1221386567 @default.
- W4379797841 cites W1514728968 @default.
- W4379797841 cites W1517224738 @default.
- W4379797841 cites W1606153735 @default.
- W4379797841 cites W1921830981 @default.
- W4379797841 cites W1963974108 @default.
- W4379797841 cites W1968410649 @default.
- W4379797841 cites W1969989079 @default.
- W4379797841 cites W1974284994 @default.
- W4379797841 cites W1987282679 @default.
- W4379797841 cites W1988062176 @default.
- W4379797841 cites W1988949059 @default.
- W4379797841 cites W1992683649 @default.
- W4379797841 cites W1994261891 @default.
- W4379797841 cites W1997562369 @default.
- W4379797841 cites W2003105919 @default.
- W4379797841 cites W2006573055 @default.
- W4379797841 cites W2015271663 @default.
- W4379797841 cites W2020100815 @default.
- W4379797841 cites W2020856504 @default.
- W4379797841 cites W2026982131 @default.
- W4379797841 cites W2033721970 @default.
- W4379797841 cites W2038132108 @default.
- W4379797841 cites W2038256717 @default.
- W4379797841 cites W2053490823 @default.
- W4379797841 cites W2055267718 @default.
- W4379797841 cites W2058252718 @default.
- W4379797841 cites W2062577852 @default.
- W4379797841 cites W2074090227 @default.
- W4379797841 cites W2074910209 @default.
- W4379797841 cites W2076329732 @default.
- W4379797841 cites W2078180525 @default.
- W4379797841 cites W2082384143 @default.
- W4379797841 cites W2100190822 @default.
- W4379797841 cites W2101294294 @default.
- W4379797841 cites W2103038967 @default.
- W4379797841 cites W2108738397 @default.
- W4379797841 cites W2109515998 @default.
- W4379797841 cites W2122679295 @default.
- W4379797841 cites W2131643797 @default.
- W4379797841 cites W2132879814 @default.
- W4379797841 cites W2140821017 @default.
- W4379797841 cites W2147293395 @default.
- W4379797841 cites W2148329006 @default.
- W4379797841 cites W2150977796 @default.
- W4379797841 cites W2153877336 @default.
- W4379797841 cites W2156723472 @default.
- W4379797841 cites W2163523836 @default.
- W4379797841 cites W2239360447 @default.
- W4379797841 cites W2302559305 @default.
- W4379797841 cites W2346962089 @default.
- W4379797841 cites W2397536361 @default.
- W4379797841 cites W2466709382 @default.
- W4379797841 cites W2473723747 @default.
- W4379797841 cites W2562169255 @default.
- W4379797841 cites W2749907022 @default.
- W4379797841 cites W2752309968 @default.
- W4379797841 cites W2754376180 @default.
- W4379797841 cites W2894479533 @default.
- W4379797841 cites W2950733362 @default.
- W4379797841 cites W2996302067 @default.
- W4379797841 cites W3000904600 @default.
- W4379797841 cites W3012911913 @default.
- W4379797841 cites W3035559789 @default.
- W4379797841 cites W3044661214 @default.
- W4379797841 cites W3129747048 @default.
- W4379797841 cites W3149757220 @default.
- W4379797841 cites W4205210465 @default.
- W4379797841 cites W4207033998 @default.
- W4379797841 cites W4250628706 @default.
- W4379797841 cites W4280652587 @default.
- W4379797841 cites W4284669952 @default.
- W4379797841 cites W4285086324 @default.
- W4379797841 cites W4309484842 @default.
- W4379797841 cites W4360938360 @default.
- W4379797841 cites W859753841 @default.
- W4379797841 doi "https://doi.org/10.1002/cctc.202300371" @default.
- W4379797841 hasPublicationYear "2023" @default.
- W4379797841 type Work @default.
- W4379797841 citedByCount "0" @default.
- W4379797841 crossrefType "journal-article" @default.
- W4379797841 hasAuthorship W4379797841A5022981478 @default.
- W4379797841 hasAuthorship W4379797841A5026188070 @default.
- W4379797841 hasAuthorship W4379797841A5030580114 @default.
- W4379797841 hasAuthorship W4379797841A5092113149 @default.
- W4379797841 hasBestOaLocation W43797978411 @default.
- W4379797841 hasConcept C111368507 @default.
- W4379797841 hasConcept C127313418 @default.
- W4379797841 hasConcept C151730666 @default.
- W4379797841 hasConcept C161790260 @default.
- W4379797841 hasConcept C165474305 @default.