Matches in SemOpenAlex for { <https://semopenalex.org/work/W4379932017> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W4379932017 endingPage "e204" @default.
- W4379932017 startingPage "e204" @default.
- W4379932017 abstract "Objective: To perform transcriptomic analysis of RNA samples extracted from urinary extracellular vesicles (uEVs) of patients with primary aldosteronism (PA), subtype aldosterone-producing adenoma (APA), before and after adrenalectomy, and patients with essential hypertension (EH). Then, to compare the differentially expressed genes (DEGs) for evaluating gene expression differences between PA and EH, and PA before and after adrenalectomy. Design and method: Urinary extracellular vesicles were isolated using a commercially available precipitating reagent and differential centrifugations. RNA libraries were prepared according to the TruSeq Stranded Total RNA protocol and sequenced by NextSeq System (Illumina). Results: Gene set enrichment analysis on DEGs in 5 patients with PA compared to 8 EH subjects revealed several significantly overrepresented classes of transcripts, including ion binding, sodium ion transmembrane transport and collagen-containing extracellular matrix. Among the more interesting genes, we found some associated to the activity of voltage-dependent ion channels (CACNA2D1, CACNA2D4, CACNG2, SCN3A, SCN5A, SCN9A, SCN11A). Moreover, in order to identify potentially aldosterone-regulated genes, we focused on DEGs in common between the comparisons APA pre-surgery vs EH and APA pre surgery vs APA post-surgery. We found, among DEGs shared across these two groups, some transcripts involved in the regulation of extracellular matrix remodeling and Phosphoinositide 3-kinases pathway (CST3, EPHB2, ITGA5, PIK3R2). Conclusions: This study contributes to a more precise characterization of the molecular mechanisms underlying PA. To our knowledge, this is the first transcriptomic study on uEVs from patients with PA and EH, confirming evidence found in other studies on different tissues (biological fluids and adrenal tissue). Our results suggest that the mRNA content of these vesicles may reflect the pathophysiological processes occurring in the urinary tract and especially in the kidney." @default.
- W4379932017 created "2023-06-09" @default.
- W4379932017 creator A5035621379 @default.
- W4379932017 creator A5041306142 @default.
- W4379932017 creator A5041970756 @default.
- W4379932017 creator A5048405840 @default.
- W4379932017 creator A5049709176 @default.
- W4379932017 creator A5056547250 @default.
- W4379932017 creator A5057941364 @default.
- W4379932017 creator A5063650307 @default.
- W4379932017 creator A5073467576 @default.
- W4379932017 creator A5075472338 @default.
- W4379932017 creator A5092120328 @default.
- W4379932017 creator A5092120329 @default.
- W4379932017 date "2023-06-01" @default.
- W4379932017 modified "2023-09-25" @default.
- W4379932017 title "TRANSCRIPTOMIC ANALYSIS OF URINARY EXTRACELLULAR VESICLES IN HYPERTENSIVE PATIENTS WITH PRIMARY ALDOSTERONISM" @default.
- W4379932017 doi "https://doi.org/10.1097/01.hjh.0000941036.83712.a7" @default.
- W4379932017 hasPublicationYear "2023" @default.
- W4379932017 type Work @default.
- W4379932017 citedByCount "0" @default.
- W4379932017 crossrefType "journal-article" @default.
- W4379932017 hasAuthorship W4379932017A5035621379 @default.
- W4379932017 hasAuthorship W4379932017A5041306142 @default.
- W4379932017 hasAuthorship W4379932017A5041970756 @default.
- W4379932017 hasAuthorship W4379932017A5048405840 @default.
- W4379932017 hasAuthorship W4379932017A5049709176 @default.
- W4379932017 hasAuthorship W4379932017A5056547250 @default.
- W4379932017 hasAuthorship W4379932017A5057941364 @default.
- W4379932017 hasAuthorship W4379932017A5063650307 @default.
- W4379932017 hasAuthorship W4379932017A5073467576 @default.
- W4379932017 hasAuthorship W4379932017A5075472338 @default.
- W4379932017 hasAuthorship W4379932017A5092120328 @default.
- W4379932017 hasAuthorship W4379932017A5092120329 @default.
- W4379932017 hasBestOaLocation W43799320171 @default.
- W4379932017 hasConcept C104317684 @default.
- W4379932017 hasConcept C126322002 @default.
- W4379932017 hasConcept C134018914 @default.
- W4379932017 hasConcept C150194340 @default.
- W4379932017 hasConcept C162317418 @default.
- W4379932017 hasConcept C189165786 @default.
- W4379932017 hasConcept C2778525890 @default.
- W4379932017 hasConcept C2780233176 @default.
- W4379932017 hasConcept C28406088 @default.
- W4379932017 hasConcept C55493867 @default.
- W4379932017 hasConcept C67705224 @default.
- W4379932017 hasConcept C71924100 @default.
- W4379932017 hasConcept C86803240 @default.
- W4379932017 hasConcept C95444343 @default.
- W4379932017 hasConceptScore W4379932017C104317684 @default.
- W4379932017 hasConceptScore W4379932017C126322002 @default.
- W4379932017 hasConceptScore W4379932017C134018914 @default.
- W4379932017 hasConceptScore W4379932017C150194340 @default.
- W4379932017 hasConceptScore W4379932017C162317418 @default.
- W4379932017 hasConceptScore W4379932017C189165786 @default.
- W4379932017 hasConceptScore W4379932017C2778525890 @default.
- W4379932017 hasConceptScore W4379932017C2780233176 @default.
- W4379932017 hasConceptScore W4379932017C28406088 @default.
- W4379932017 hasConceptScore W4379932017C55493867 @default.
- W4379932017 hasConceptScore W4379932017C67705224 @default.
- W4379932017 hasConceptScore W4379932017C71924100 @default.
- W4379932017 hasConceptScore W4379932017C86803240 @default.
- W4379932017 hasConceptScore W4379932017C95444343 @default.
- W4379932017 hasIssue "Suppl 3" @default.
- W4379932017 hasLocation W43799320171 @default.
- W4379932017 hasOpenAccess W4379932017 @default.
- W4379932017 hasPrimaryLocation W43799320171 @default.
- W4379932017 hasRelatedWork W1970251377 @default.
- W4379932017 hasRelatedWork W1973661188 @default.
- W4379932017 hasRelatedWork W1993396385 @default.
- W4379932017 hasRelatedWork W2007708517 @default.
- W4379932017 hasRelatedWork W2012265513 @default.
- W4379932017 hasRelatedWork W2043121195 @default.
- W4379932017 hasRelatedWork W2101834938 @default.
- W4379932017 hasRelatedWork W2148607670 @default.
- W4379932017 hasRelatedWork W2176052578 @default.
- W4379932017 hasRelatedWork W2296889994 @default.
- W4379932017 hasVolume "41" @default.
- W4379932017 isParatext "false" @default.
- W4379932017 isRetracted "false" @default.
- W4379932017 workType "article" @default.