Matches in SemOpenAlex for { <https://semopenalex.org/work/W4380033685> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W4380033685 endingPage "1397" @default.
- W4380033685 startingPage "1391" @default.
- W4380033685 abstract "Spotted-wing Drosophila, Drosophila suzukii, is an economically important pest of small fruits worldwide. Currently, the timing of management strategies relies on detection of adult flies captured in baited monitoring traps; however, identifying D. suzukii in trap catch based on morphology can be challenging for growers. DNA-based diagnostic methods such as loop-mediated isothermal amplification (LAMP) have the potential to improve D. suzukii detection. This study evaluated a LAMP assay as a diagnostic tool to discriminate between D. suzukii and closely related drosophilid species found commonly in monitoring traps in the Midwestern United States. Targeting the mitochondrial cytochrome oxidase I (COI) gene, we found the LAMP assay accurately detected D. suzukii with as little as 0.1 ng/μl of DNA at 63 °C for 50 min. Under these optimal incubation conditions, D. suzukii could be discriminated from D. affinis and D. simulans consistently, when specimens collected from liquid monitoring traps were tested independently. Compared to other DNA-based diagnostic tools for D. suzukii, LAMP offers unique benefits: DNA extraction is not required, testing occurs at one temperature in less than 1 h, and positive results are visible as a colorimetric change from pink to yellow. The LAMP assay for D. suzukii can reduce reliance on morphological identification, enhance the adoption of monitoring tools, and improve accuracy of detection. Further optimization can be conducted to evaluate the accuracy and sensitivity of results when a mixture of DNA from both D. suzukii and congener flies are tested in a single LAMP reaction." @default.
- W4380033685 created "2023-06-10" @default.
- W4380033685 creator A5063985116 @default.
- W4380033685 creator A5083190053 @default.
- W4380033685 creator A5092124885 @default.
- W4380033685 date "2023-06-10" @default.
- W4380033685 modified "2023-09-25" @default.
- W4380033685 title "Optimizing a rapid LAMP assay for discrimination of <i>Drosophila suzukii</i> (Diptera: Drosophilidae) from common drosophilids captured in monitoring traps from the Midwest, United States" @default.
- W4380033685 cites W1870652839 @default.
- W4380033685 cites W1963670684 @default.
- W4380033685 cites W2022087555 @default.
- W4380033685 cites W2044723475 @default.
- W4380033685 cites W2069640202 @default.
- W4380033685 cites W2088077177 @default.
- W4380033685 cites W2097298673 @default.
- W4380033685 cites W2102494588 @default.
- W4380033685 cites W2105275554 @default.
- W4380033685 cites W2106911290 @default.
- W4380033685 cites W2107308375 @default.
- W4380033685 cites W2141859468 @default.
- W4380033685 cites W2152183313 @default.
- W4380033685 cites W2163191590 @default.
- W4380033685 cites W2163622045 @default.
- W4380033685 cites W2173143639 @default.
- W4380033685 cites W2257625520 @default.
- W4380033685 cites W2548269698 @default.
- W4380033685 cites W2606634398 @default.
- W4380033685 cites W2607503614 @default.
- W4380033685 cites W2744737860 @default.
- W4380033685 cites W2770361968 @default.
- W4380033685 cites W2791647297 @default.
- W4380033685 cites W2800166912 @default.
- W4380033685 cites W2942279931 @default.
- W4380033685 cites W3135074581 @default.
- W4380033685 cites W3142918549 @default.
- W4380033685 cites W3195441893 @default.
- W4380033685 cites W3200065029 @default.
- W4380033685 cites W3214909687 @default.
- W4380033685 cites W4206991433 @default.
- W4380033685 cites W4207016523 @default.
- W4380033685 cites W4235157105 @default.
- W4380033685 cites W4303858698 @default.
- W4380033685 doi "https://doi.org/10.1093/jee/toad105" @default.
- W4380033685 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37300369" @default.
- W4380033685 hasPublicationYear "2023" @default.
- W4380033685 type Work @default.
- W4380033685 citedByCount "0" @default.
- W4380033685 crossrefType "journal-article" @default.
- W4380033685 hasAuthorship W4380033685A5063985116 @default.
- W4380033685 hasAuthorship W4380033685A5083190053 @default.
- W4380033685 hasAuthorship W4380033685A5092124885 @default.
- W4380033685 hasConcept C104317684 @default.
- W4380033685 hasConcept C144027150 @default.
- W4380033685 hasConcept C22508944 @default.
- W4380033685 hasConcept C2776041557 @default.
- W4380033685 hasConcept C2777379243 @default.
- W4380033685 hasConcept C2777965303 @default.
- W4380033685 hasConcept C2780104201 @default.
- W4380033685 hasConcept C54355233 @default.
- W4380033685 hasConcept C552990157 @default.
- W4380033685 hasConcept C60635243 @default.
- W4380033685 hasConcept C86803240 @default.
- W4380033685 hasConceptScore W4380033685C104317684 @default.
- W4380033685 hasConceptScore W4380033685C144027150 @default.
- W4380033685 hasConceptScore W4380033685C22508944 @default.
- W4380033685 hasConceptScore W4380033685C2776041557 @default.
- W4380033685 hasConceptScore W4380033685C2777379243 @default.
- W4380033685 hasConceptScore W4380033685C2777965303 @default.
- W4380033685 hasConceptScore W4380033685C2780104201 @default.
- W4380033685 hasConceptScore W4380033685C54355233 @default.
- W4380033685 hasConceptScore W4380033685C552990157 @default.
- W4380033685 hasConceptScore W4380033685C60635243 @default.
- W4380033685 hasConceptScore W4380033685C86803240 @default.
- W4380033685 hasFunder F4320332299 @default.
- W4380033685 hasIssue "4" @default.
- W4380033685 hasLocation W43800336851 @default.
- W4380033685 hasLocation W43800336852 @default.
- W4380033685 hasOpenAccess W4380033685 @default.
- W4380033685 hasPrimaryLocation W43800336851 @default.
- W4380033685 hasRelatedWork W1194893348 @default.
- W4380033685 hasRelatedWork W2002666526 @default.
- W4380033685 hasRelatedWork W2095523379 @default.
- W4380033685 hasRelatedWork W21063387 @default.
- W4380033685 hasRelatedWork W2107308375 @default.
- W4380033685 hasRelatedWork W2153569171 @default.
- W4380033685 hasRelatedWork W2548269698 @default.
- W4380033685 hasRelatedWork W2804036555 @default.
- W4380033685 hasRelatedWork W3186218731 @default.
- W4380033685 hasRelatedWork W4367397510 @default.
- W4380033685 hasVolume "116" @default.
- W4380033685 isParatext "false" @default.
- W4380033685 isRetracted "false" @default.
- W4380033685 workType "article" @default.