Matches in SemOpenAlex for { <https://semopenalex.org/work/W4380047162> ?p ?o ?g. }
- W4380047162 endingPage "1236" @default.
- W4380047162 startingPage "1236" @default.
- W4380047162 abstract "Peroxisomes serve as important centers for cellular redox metabolism and communication. However, fundamental gaps remain in our understanding of how the peroxisomal redox equilibrium is maintained. In particular, very little is known about the function of the nonenzymatic antioxidant glutathione in the peroxisome interior and how the glutathione antioxidant system balances with peroxisomal protein thiols. So far, only one human peroxisomal glutathione-consuming enzyme has been identified: glutathione S-transferase 1 kappa (GSTK1). To study the role of this enzyme in peroxisomal glutathione regulation and function, a GSTK1-deficient HEK-293 cell line was generated and fluorescent redox sensors were used to monitor the intraperoxisomal GSSG/GSH and NAD+/NADH redox couples and NADPH levels. We provide evidence that ablation of GSTK1 does not change the basal intraperoxisomal redox state but significantly extends the recovery period of the peroxisomal glutathione redox sensor po-roGFP2 upon treatment of the cells with thiol-specific oxidants. Given that this delay (i) can be rescued by reintroduction of GSTK1, but not its S16A active site mutant, and (ii) is not observed with a glutaredoxin-tagged version of po-roGFP2, our findings demonstrate that GSTK1 contains GSH-dependent disulfide bond oxidoreductase activity." @default.
- W4380047162 created "2023-06-10" @default.
- W4380047162 creator A5017893923 @default.
- W4380047162 creator A5021844928 @default.
- W4380047162 creator A5041588764 @default.
- W4380047162 creator A5056857018 @default.
- W4380047162 creator A5057865344 @default.
- W4380047162 creator A5077954463 @default.
- W4380047162 creator A5080201137 @default.
- W4380047162 date "2023-06-07" @default.
- W4380047162 modified "2023-10-16" @default.
- W4380047162 title "Functional Analysis of GSTK1 in Peroxisomal Redox Homeostasis in HEK-293 Cells" @default.
- W4380047162 cites W1600553067 @default.
- W4380047162 cites W1766442549 @default.
- W4380047162 cites W1773516573 @default.
- W4380047162 cites W1894322524 @default.
- W4380047162 cites W1930548389 @default.
- W4380047162 cites W1969120823 @default.
- W4380047162 cites W1971542373 @default.
- W4380047162 cites W1974060212 @default.
- W4380047162 cites W1977709885 @default.
- W4380047162 cites W1978240280 @default.
- W4380047162 cites W1981123464 @default.
- W4380047162 cites W1985069778 @default.
- W4380047162 cites W1993504015 @default.
- W4380047162 cites W1995680796 @default.
- W4380047162 cites W1997161797 @default.
- W4380047162 cites W2002269806 @default.
- W4380047162 cites W2008760489 @default.
- W4380047162 cites W2016054170 @default.
- W4380047162 cites W2027606345 @default.
- W4380047162 cites W2031652215 @default.
- W4380047162 cites W2042208218 @default.
- W4380047162 cites W2044802013 @default.
- W4380047162 cites W2045631219 @default.
- W4380047162 cites W2046855853 @default.
- W4380047162 cites W2066915426 @default.
- W4380047162 cites W2085413297 @default.
- W4380047162 cites W2085564139 @default.
- W4380047162 cites W2094776252 @default.
- W4380047162 cites W2099540110 @default.
- W4380047162 cites W2101288748 @default.
- W4380047162 cites W2101641567 @default.
- W4380047162 cites W2104491557 @default.
- W4380047162 cites W2111781163 @default.
- W4380047162 cites W2117176829 @default.
- W4380047162 cites W2122214165 @default.
- W4380047162 cites W2138135526 @default.
- W4380047162 cites W2142645505 @default.
- W4380047162 cites W2155345385 @default.
- W4380047162 cites W2156905115 @default.
- W4380047162 cites W2160071206 @default.
- W4380047162 cites W2165301921 @default.
- W4380047162 cites W2165723638 @default.
- W4380047162 cites W2168024298 @default.
- W4380047162 cites W2267828081 @default.
- W4380047162 cites W2273624704 @default.
- W4380047162 cites W2275001158 @default.
- W4380047162 cites W2297635630 @default.
- W4380047162 cites W2439947947 @default.
- W4380047162 cites W2465049570 @default.
- W4380047162 cites W2549188597 @default.
- W4380047162 cites W2622229743 @default.
- W4380047162 cites W2623012019 @default.
- W4380047162 cites W2722324655 @default.
- W4380047162 cites W2765921352 @default.
- W4380047162 cites W2784311670 @default.
- W4380047162 cites W2791136219 @default.
- W4380047162 cites W2794039477 @default.
- W4380047162 cites W2805752574 @default.
- W4380047162 cites W2885750535 @default.
- W4380047162 cites W2898850054 @default.
- W4380047162 cites W2904626484 @default.
- W4380047162 cites W2945345732 @default.
- W4380047162 cites W2965179790 @default.
- W4380047162 cites W2976021699 @default.
- W4380047162 cites W2994436123 @default.
- W4380047162 cites W3012033770 @default.
- W4380047162 cites W3012319668 @default.
- W4380047162 cites W3014696785 @default.
- W4380047162 cites W3093053585 @default.
- W4380047162 cites W3094678257 @default.
- W4380047162 cites W3118674668 @default.
- W4380047162 cites W3156453517 @default.
- W4380047162 cites W3158503443 @default.
- W4380047162 cites W3214966814 @default.
- W4380047162 cites W4213260574 @default.
- W4380047162 cites W4214646780 @default.
- W4380047162 cites W4238581030 @default.
- W4380047162 cites W4291020935 @default.
- W4380047162 cites W4303857481 @default.
- W4380047162 cites W4318393440 @default.
- W4380047162 cites W4322769922 @default.
- W4380047162 cites W4360599484 @default.
- W4380047162 cites W4360600971 @default.
- W4380047162 doi "https://doi.org/10.3390/antiox12061236" @default.
- W4380047162 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37371965" @default.
- W4380047162 hasPublicationYear "2023" @default.