Matches in SemOpenAlex for { <https://semopenalex.org/work/W4380048612> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W4380048612 endingPage "965" @default.
- W4380048612 startingPage "959" @default.
- W4380048612 abstract "Purpose: To evaluate the contribution of collectin subfamily member 12 (COLEC12) in insulin resistance associated with gestational diabetes mellitus (GDM).
 Methods: Insulin resistance was induced in human placental trophoblast (HTR-8/SVneo) cells in vitro by incubating them with 1 μM insulin for 48 h. Expression of COLEC12 was assessed using western blot and quantitative real time-polymerase chain reaction (qRT-PCR). Distribution of glucose transporters GLUT1 and GLUT4 was visualized using immunofluorescent staining. Furthermore, the level of reactive oxygen species (ROS) was evaluated by flow cytometry.
 Results: Insulin-resistant HTR-8/SVneo (IR-HTR-8/SVneo) cells showed significantly elevated COLEC12 expression (p < 0.001). Knocking down of COLEC12 in these cells led to a significant reduction in GLUT1 expression, and increase in both glucose consumption and GLUT4 expression (p < 0.001). Furthermore, COLEC12 knockdown levels of ROS increased SOD expression in IR-HTR8/SVneo, while knockdown of COLEC12 reduced p-insulin receptor substrate (IRS) (Ser307) protein expression, but increased p-IRS-1 (Tyr896), p-insulin receptor β (IRβ) (Tyr1361), and p-AKT levels. COLEC12 knockdown also decreased cyclooxygenase-2 (COX-2) expression, resulting in lower prostaglandin E2 (PGE2) levels in IR-HTR-8/SVneo. COLEC12 over-expression attenuated celecoxibinduced increase in glucose consumption, resulting in decreased COX2 and PGE2 levels.
 Conclusion: COLEC12 in IR-HTR-8/SVneo cells has antioxidant effects that protect against insulin resistance. This protective effect is achieved through the down-regulation of COX-2/PGE2. These findings provide a potential strategy for the treatment of GDM." @default.
- W4380048612 created "2023-06-10" @default.
- W4380048612 creator A5010479652 @default.
- W4380048612 creator A5011757099 @default.
- W4380048612 creator A5071891848 @default.
- W4380048612 creator A5084573104 @default.
- W4380048612 date "2023-06-05" @default.
- W4380048612 modified "2023-09-30" @default.
- W4380048612 title "COLEC12 expression participates in trophoblast insulin resistance, and reverses celecoxib-mediated inhibition of COX2-PGE2 axis in gestational diabetes" @default.
- W4380048612 doi "https://doi.org/10.4314/tjpr.v22i5.4" @default.
- W4380048612 hasPublicationYear "2023" @default.
- W4380048612 type Work @default.
- W4380048612 citedByCount "0" @default.
- W4380048612 crossrefType "journal-article" @default.
- W4380048612 hasAuthorship W4380048612A5010479652 @default.
- W4380048612 hasAuthorship W4380048612A5011757099 @default.
- W4380048612 hasAuthorship W4380048612A5071891848 @default.
- W4380048612 hasAuthorship W4380048612A5084573104 @default.
- W4380048612 hasBestOaLocation W43800486121 @default.
- W4380048612 hasConcept C126322002 @default.
- W4380048612 hasConcept C134018914 @default.
- W4380048612 hasConcept C161573976 @default.
- W4380048612 hasConcept C172680121 @default.
- W4380048612 hasConcept C173396325 @default.
- W4380048612 hasConcept C185592680 @default.
- W4380048612 hasConcept C190283241 @default.
- W4380048612 hasConcept C2776188179 @default.
- W4380048612 hasConcept C2776953305 @default.
- W4380048612 hasConcept C2777391703 @default.
- W4380048612 hasConcept C2777495146 @default.
- W4380048612 hasConcept C2777952866 @default.
- W4380048612 hasConcept C2779234561 @default.
- W4380048612 hasConcept C2779306644 @default.
- W4380048612 hasConcept C54355233 @default.
- W4380048612 hasConcept C55493867 @default.
- W4380048612 hasConcept C71924100 @default.
- W4380048612 hasConcept C86803240 @default.
- W4380048612 hasConceptScore W4380048612C126322002 @default.
- W4380048612 hasConceptScore W4380048612C134018914 @default.
- W4380048612 hasConceptScore W4380048612C161573976 @default.
- W4380048612 hasConceptScore W4380048612C172680121 @default.
- W4380048612 hasConceptScore W4380048612C173396325 @default.
- W4380048612 hasConceptScore W4380048612C185592680 @default.
- W4380048612 hasConceptScore W4380048612C190283241 @default.
- W4380048612 hasConceptScore W4380048612C2776188179 @default.
- W4380048612 hasConceptScore W4380048612C2776953305 @default.
- W4380048612 hasConceptScore W4380048612C2777391703 @default.
- W4380048612 hasConceptScore W4380048612C2777495146 @default.
- W4380048612 hasConceptScore W4380048612C2777952866 @default.
- W4380048612 hasConceptScore W4380048612C2779234561 @default.
- W4380048612 hasConceptScore W4380048612C2779306644 @default.
- W4380048612 hasConceptScore W4380048612C54355233 @default.
- W4380048612 hasConceptScore W4380048612C55493867 @default.
- W4380048612 hasConceptScore W4380048612C71924100 @default.
- W4380048612 hasConceptScore W4380048612C86803240 @default.
- W4380048612 hasIssue "5" @default.
- W4380048612 hasLocation W43800486121 @default.
- W4380048612 hasOpenAccess W4380048612 @default.
- W4380048612 hasPrimaryLocation W43800486121 @default.
- W4380048612 hasRelatedWork W1988699731 @default.
- W4380048612 hasRelatedWork W2069349303 @default.
- W4380048612 hasRelatedWork W2087127797 @default.
- W4380048612 hasRelatedWork W2102654574 @default.
- W4380048612 hasRelatedWork W2114435268 @default.
- W4380048612 hasRelatedWork W2410784692 @default.
- W4380048612 hasRelatedWork W3032853368 @default.
- W4380048612 hasRelatedWork W3113810870 @default.
- W4380048612 hasRelatedWork W4232601999 @default.
- W4380048612 hasRelatedWork W4255402764 @default.
- W4380048612 hasVolume "22" @default.
- W4380048612 isParatext "false" @default.
- W4380048612 isRetracted "false" @default.
- W4380048612 workType "article" @default.