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- W4380049063 abstract "Paenibacillus dendritiformis UJA2219 isolated from carrot produces broad-spectrum antibacterial activity. The aim of the present study was to determine the impact of partially-purified cell-culture extracts of strain UJA2219 on the microbial load and bacterial diversity of a homemade vegetable puree. The puree was challenged with an overnight culture of strain UJA2219 or with cultured broth extracts partially purified by cation exchange (CE) chromatography or reversed-phase (RP) chromatography and incubated for 7 days at temperatures of 4 °C or 25 °C. The best results were obtained at 25 °C with the RP extract, decreasing counts of the presumptive Enterobacteriaceae below detectable levels. The bacterial diversity of control and treated puree was studied by Illumina paired-end sequencing, using DNA extracted from the puree samples incubated at 25 °C for 24 h. The controls and the puree inoculated with the UJA2219 strain showed an almost-identical bacterial diversity profile, with Pseudomonadota (mainly Fam. Pseudomonadaceae -gen. Pseudomonas- and Enterobacteriace as the most abundant groups). The greatest differences in bacterial diversity were obtained in the puree treated with RP extract, showing a decrease in the relative abundance of Pseudomonadota (especially gen. Pseudomonas) and an increase of Bacillota (mainly of the genera Bacillus, Enterococcus and Lactococcus). Results from the study suggest that the antimicrobial preparations from strain UJA2219 have a potential for application in food biopreservation." @default.
- W4380049063 created "2023-06-10" @default.
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- W4380049063 date "2023-06-07" @default.
- W4380049063 modified "2023-09-27" @default.
- W4380049063 title "Antimicrobial Activity and Biodiversity Study of a Homemade Vegetable Puree Treated with Antimicrobials from Paenibacillus dendritiformis" @default.
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- W4380049063 doi "https://doi.org/10.3390/app13126901" @default.
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