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• W4380090087 abstract "Bacterial extracellular vesicles (EVs) are natural lipidic nanoparticles implicated in intercellular communication. Although EV research focused mainly on pathogens, the interest in probiotic-derived EVs is now rising. One example is Propionibacterium freudenreichii, which produces EVs with anti-inflammatory effects on human epithelial cells. Our previous study with P. freudenreichii showed that EVs purified by size exclusion chromatography (SEC) displayed variations in protein content according to bacterial growth conditions. Considering these content variations, we hypothesized that a comparative proteomic analysis of EVs recovered in different conditions would elucidate whether a representative vesicular proteome existed, possibly providing a robust proteome dataset for further analysis. Therefore, P. freudenreichii was grown in two culture media, and EVs were purified by sucrose density gradient ultracentrifugation (UC). Microscopic and size characterization confirmed EV purification, while shotgun proteomics unveiled that they carried a diverse set of proteins. A comparative analysis of the protein content of UC- and SEC-derived EVs, isolated from cultures either in UF (cow milk ultrafiltrate medium) or YEL (laboratory yeast extract lactate medium), showed that EVs from all these conditions shared 308 proteins. This EV core proteome was notably enriched in proteins related to immunomodulation. Moreover, it showed distinctive features, including highly interacting proteins, compositional biases for some specific amino acids, and other biochemical parameters. Overall, this work broadens the toolset for the purification of P. freudenreichii-derived EVs, identifies a representative vesicular proteome, and enumerates conserved features in vesicular proteins. These results hold the potential for providing candidate biomarkers of purification quality, and insights into the mechanisms of EV biogenesis and cargo sorting." @default.
• W4380090087 created "2023-06-10" @default.
• W4380090087 creator A5000482298 @default.
• W4380090087 creator A5002568944 @default.
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• W4380090087 creator A5009401988 @default.
• W4380090087 creator A5025907932 @default.
• W4380090087 creator A5049435933 @default.
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• W4380090087 creator A5072772380 @default.
• W4380090087 creator A5088644771 @default.
• W4380090087 creator A5090969979 @default.
• W4380090087 date "2023-01-01" @default.
• W4380090087 modified "2023-10-15" @default.
• W4380090087 title "Different culture media and purification methods unveil the core proteome of <i>Propionibacterium freudenreichii</i>-derived extracellular vesicles" @default.
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