Matches in SemOpenAlex for { <https://semopenalex.org/work/W4380264598> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W4380264598 abstract "Introdutcion: The Zika virus (ZIKV) infections are a healthcare concern mostly in the Americas, Africa, and Asia but have increased its endemicity area beyond these geographical regions. Due to the advances in infections by Zika virus, it is imperative to develop diagnostic and preventive tools against this viral agent. Virus-like particles (VLPs) appear as a suitable approach for use as antiviral vaccines. Methods: In this work, a methodology was established to produce virus-like particles containing the structural proteins, C, prM, and E of Zika virus produced in insect cells using the gene expression system derived from baculovirus. The vector pFast- CprME -ZIKV was constructed containing the gene sequences of Zika virus structural proteins and it was used to generate the recombinant bacmids (Bac- CprME -ZIKV) through transformation into DH10BacTM cells. The Bac- CprME -ZIKV was transfected in Spodoptera frugiperda (Sf9) insect cells and batches of BV- CprME -ZIKV were obtained by infection assays using a multiplicity of infection of 2. The Sf9 cells were infected, and the supernatant was collected 96 h post-infection. The expression of the CprME -ZIKV protein on the cell surface could be observed by immunochemical assays. To concentrate and purify virus-like particles, the sucrose and iodixanol gradients were evaluated, and the correct CprME -ZIKV proteins' conformation was evaluated by the Western blot assay. The virus-like particles were also analyzed and characterized by transmission electron microscopy. Results and discussion: Spherical structures like the native Zika virus from 50 to 65 nm containing the CprME -ZIKV proteins on their surface were observed in micrographs. The results obtained can be useful in the development path for a vaccine candidate against Zika virus." @default.
- W4380264598 created "2023-06-12" @default.
- W4380264598 creator A5024986284 @default.
- W4380264598 creator A5034813254 @default.
- W4380264598 creator A5039641731 @default.
- W4380264598 creator A5042237704 @default.
- W4380264598 creator A5049636709 @default.
- W4380264598 creator A5057254719 @default.
- W4380264598 creator A5064419688 @default.
- W4380264598 creator A5081395880 @default.
- W4380264598 date "2023-06-12" @default.
- W4380264598 modified "2023-09-29" @default.
- W4380264598 title "Zika virus-like particles (VLPs) produced in insect cells" @default.
- W4380264598 cites W1558143285 @default.
- W4380264598 cites W1827825760 @default.
- W4380264598 cites W1971347315 @default.
- W4380264598 cites W1973619678 @default.
- W4380264598 cites W1979001015 @default.
- W4380264598 cites W2029673828 @default.
- W4380264598 cites W2037215631 @default.
- W4380264598 cites W2039207244 @default.
- W4380264598 cites W2046182783 @default.
- W4380264598 cites W2119292034 @default.
- W4380264598 cites W2122349387 @default.
- W4380264598 cites W2138527314 @default.
- W4380264598 cites W2149913957 @default.
- W4380264598 cites W2165960748 @default.
- W4380264598 cites W2234047240 @default.
- W4380264598 cites W2265557890 @default.
- W4380264598 cites W2271569098 @default.
- W4380264598 cites W2346936410 @default.
- W4380264598 cites W2404313081 @default.
- W4380264598 cites W2465809358 @default.
- W4380264598 cites W2613273883 @default.
- W4380264598 cites W2741681003 @default.
- W4380264598 cites W2753483322 @default.
- W4380264598 cites W2753948213 @default.
- W4380264598 cites W2803237484 @default.
- W4380264598 cites W3029760314 @default.
- W4380264598 doi "https://doi.org/10.3389/fphar.2023.1181566" @default.
- W4380264598 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37377933" @default.
- W4380264598 hasPublicationYear "2023" @default.
- W4380264598 type Work @default.
- W4380264598 citedByCount "0" @default.
- W4380264598 crossrefType "journal-article" @default.
- W4380264598 hasAuthorship W4380264598A5024986284 @default.
- W4380264598 hasAuthorship W4380264598A5034813254 @default.
- W4380264598 hasAuthorship W4380264598A5039641731 @default.
- W4380264598 hasAuthorship W4380264598A5042237704 @default.
- W4380264598 hasAuthorship W4380264598A5049636709 @default.
- W4380264598 hasAuthorship W4380264598A5057254719 @default.
- W4380264598 hasAuthorship W4380264598A5064419688 @default.
- W4380264598 hasAuthorship W4380264598A5081395880 @default.
- W4380264598 hasBestOaLocation W43802645981 @default.
- W4380264598 hasConcept C103878061 @default.
- W4380264598 hasConcept C104317684 @default.
- W4380264598 hasConcept C159047783 @default.
- W4380264598 hasConcept C203233990 @default.
- W4380264598 hasConcept C2522874641 @default.
- W4380264598 hasConcept C2776387124 @default.
- W4380264598 hasConcept C2776415932 @default.
- W4380264598 hasConcept C2777053367 @default.
- W4380264598 hasConcept C2777773057 @default.
- W4380264598 hasConcept C2780568436 @default.
- W4380264598 hasConcept C40767141 @default.
- W4380264598 hasConcept C54355233 @default.
- W4380264598 hasConcept C86803240 @default.
- W4380264598 hasConceptScore W4380264598C103878061 @default.
- W4380264598 hasConceptScore W4380264598C104317684 @default.
- W4380264598 hasConceptScore W4380264598C159047783 @default.
- W4380264598 hasConceptScore W4380264598C203233990 @default.
- W4380264598 hasConceptScore W4380264598C2522874641 @default.
- W4380264598 hasConceptScore W4380264598C2776387124 @default.
- W4380264598 hasConceptScore W4380264598C2776415932 @default.
- W4380264598 hasConceptScore W4380264598C2777053367 @default.
- W4380264598 hasConceptScore W4380264598C2777773057 @default.
- W4380264598 hasConceptScore W4380264598C2780568436 @default.
- W4380264598 hasConceptScore W4380264598C40767141 @default.
- W4380264598 hasConceptScore W4380264598C54355233 @default.
- W4380264598 hasConceptScore W4380264598C86803240 @default.
- W4380264598 hasFunder F4320320997 @default.
- W4380264598 hasLocation W43802645981 @default.
- W4380264598 hasLocation W43802645982 @default.
- W4380264598 hasOpenAccess W4380264598 @default.
- W4380264598 hasPrimaryLocation W43802645981 @default.
- W4380264598 hasRelatedWork W1024660077 @default.
- W4380264598 hasRelatedWork W2096457885 @default.
- W4380264598 hasRelatedWork W2111254976 @default.
- W4380264598 hasRelatedWork W2145957435 @default.
- W4380264598 hasRelatedWork W2227378890 @default.
- W4380264598 hasRelatedWork W2369500599 @default.
- W4380264598 hasRelatedWork W2987786716 @default.
- W4380264598 hasRelatedWork W3030512877 @default.
- W4380264598 hasRelatedWork W4380264598 @default.
- W4380264598 hasRelatedWork W3100051458 @default.
- W4380264598 hasVolume "14" @default.
- W4380264598 isParatext "false" @default.
- W4380264598 isRetracted "false" @default.
- W4380264598 workType "article" @default.