FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4380320199> ?p ?o ?g. }

• W4380320199 endingPage "131836" @default.
• W4380320199 startingPage "131836" @default.
• W4380320199 abstract "Ochratoxin A (OTA) is among the most prevalent mycotoxins detected in agroproducts, posing serious threats to human and livestock health. Using enzymes to conduct OTA detoxification is an appealing potential strategy. The recently identified amidohydrolase from Stenotrophomonas acidaminiphila, termed ADH3, is the most efficient OTA-detoxifying enzyme reported thus far and can hydrolyze OTA to nontoxic ochratoxin α (OTα) and L-β-phenylalanine (Phe). To elucidate the catalytic mechanism of ADH3, we solved the single-particle cryo-electron microscopy (cryo-EM) structures of apo-form, Phe- and OTA-bound ADH3 to an overall resolution of 2.5-2.7 Å. The role of OTA-binding residues was investigated by structural, mutagenesis and biochemical analyses. We also rationally engineered ADH3 and obtained variant S88E, whose catalytic activity was elevated by 3.7-fold. Structural analysis of variant S88E indicates that the E88 side chain provides additional hydrogen bond interactions to the OTα moiety. Furthermore, the OTA-hydrolytic activity of variant S88E expressed in Pichia pastoris is comparable to that of Escherichia coli-expressed enzyme, revealing the feasibility of employing the industrial yeast strain to produce ADH3 and its variants for further applications. These results unveil a wealth of information about the catalytic mechanism of ADH3-mediated OTA degradation and provide a blueprint for rational engineering of high-efficiency OTA-detoxifying machineries." @default.
• W4380320199 created "2023-06-13" @default.
• W4380320199 creator A5011311801 @default.
• W4380320199 creator A5015001085 @default.
• W4380320199 creator A5017609052 @default.
• W4380320199 creator A5042198005 @default.
• W4380320199 creator A5047521880 @default.
• W4380320199 creator A5047843836 @default.
• W4380320199 creator A5048853410 @default.
• W4380320199 creator A5054314711 @default.
• W4380320199 creator A5054946208 @default.
• W4380320199 creator A5065157641 @default.
• W4380320199 creator A5073960918 @default.
• W4380320199 creator A5078655586 @default.
• W4380320199 date "2023-09-01" @default.
• W4380320199 modified "2023-10-18" @default.
• W4380320199 title "Cryo-EM structure and rational engineering of a superefficient ochratoxin A-detoxifying amidohydrolase" @default.
• W4380320199 cites W1968984443 @default.
• W4380320199 cites W1974057776 @default.
• W4380320199 cites W1985203726 @default.
• W4380320199 cites W1995487652 @default.
• W4380320199 cites W2004075793 @default.
• W4380320199 cites W2012268433 @default.
• W4380320199 cites W2014808512 @default.
• W4380320199 cites W2039478231 @default.
• W4380320199 cites W2060180618 @default.
• W4380320199 cites W2066239997 @default.
• W4380320199 cites W2068874946 @default.
• W4380320199 cites W2086812659 @default.
• W4380320199 cites W2109239846 @default.
• W4380320199 cites W2109427910 @default.
• W4380320199 cites W2124026197 @default.
• W4380320199 cites W2132629607 @default.
• W4380320199 cites W2154714625 @default.
• W4380320199 cites W2263423519 @default.
• W4380320199 cites W2587450650 @default.
• W4380320199 cites W2587625522 @default.
• W4380320199 cites W2735861525 @default.
• W4380320199 cites W2786390877 @default.
• W4380320199 cites W2795132833 @default.
• W4380320199 cites W2950967305 @default.
• W4380320199 cites W2972917012 @default.
• W4380320199 cites W2991280091 @default.
• W4380320199 cites W3015734973 @default.
• W4380320199 cites W3022586980 @default.
• W4380320199 cites W3138180654 @default.
• W4380320199 cites W3163135645 @default.
• W4380320199 cites W3183928083 @default.
• W4380320199 cites W3192472718 @default.
• W4380320199 cites W3202287440 @default.
• W4380320199 cites W3203900512 @default.
• W4380320199 cites W4200351294 @default.
• W4380320199 cites W4206170751 @default.
• W4380320199 cites W4206191559 @default.
• W4380320199 cites W4206397454 @default.
• W4380320199 cites W4212901488 @default.
• W4380320199 cites W4220740593 @default.
• W4380320199 cites W4242040668 @default.
• W4380320199 cites W4282922306 @default.
• W4380320199 cites W4283710656 @default.
• W4380320199 cites W4288060889 @default.
• W4380320199 cites W4289774587 @default.
• W4380320199 cites W4290831365 @default.
• W4380320199 cites W4292103489 @default.
• W4380320199 cites W4292575154 @default.
• W4380320199 cites W4295296326 @default.
• W4380320199 cites W4296242417 @default.
• W4380320199 cites W4312210393 @default.
• W4380320199 doi "https://doi.org/10.1016/j.jhazmat.2023.131836" @default.
• W4380320199 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37331057" @default.
• W4380320199 hasPublicationYear "2023" @default.
• W4380320199 type Work @default.
• W4380320199 citedByCount "0" @default.
• W4380320199 crossrefType "journal-article" @default.
• W4380320199 hasAuthorship W4380320199A5011311801 @default.
• W4380320199 hasAuthorship W4380320199A5015001085 @default.
• W4380320199 hasAuthorship W4380320199A5017609052 @default.
• W4380320199 hasAuthorship W4380320199A5042198005 @default.
• W4380320199 hasAuthorship W4380320199A5047521880 @default.
• W4380320199 hasAuthorship W4380320199A5047843836 @default.
• W4380320199 hasAuthorship W4380320199A5048853410 @default.
• W4380320199 hasAuthorship W4380320199A5054314711 @default.
• W4380320199 hasAuthorship W4380320199A5054946208 @default.
• W4380320199 hasAuthorship W4380320199A5065157641 @default.
• W4380320199 hasAuthorship W4380320199A5073960918 @default.
• W4380320199 hasAuthorship W4380320199A5078655586 @default.
• W4380320199 hasConcept C104317684 @default.
• W4380320199 hasConcept C145770059 @default.
• W4380320199 hasConcept C16318435 @default.
• W4380320199 hasConcept C181199279 @default.
• W4380320199 hasConcept C185592680 @default.
• W4380320199 hasConcept C2776008721 @default.
• W4380320199 hasConcept C2776568683 @default.
• W4380320199 hasConcept C2779485144 @default.
• W4380320199 hasConcept C2780120296 @default.
• W4380320199 hasConcept C2781372317 @default.
• W4380320199 hasConcept C31903555 @default.
• W4380320199 hasConcept C40767141 @default.

• next