Matches in SemOpenAlex for { <https://semopenalex.org/work/W4380372983> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W4380372983 endingPage "i43" @default.
- W4380372983 startingPage "i43" @default.
- W4380372983 abstract "Abstract BACKGROUND Pediatric high-grade gliomas (PHGG) are aggressive, undifferentiated central nervous system (CNS) tumors. PHGG comprises subtypes that differ phenotypically, histologically and by cellular composition. Despite intensive research, overall survival rates have remained for decades around 2% for diffuse midline glioma (DMG) and 20% for non-DMG PHGG. We hypothesized that PHGG’s heterogeneity, a key difficulty in devising therapies, results from functional and lineage differences in tumor cell types. METHODS Using 19 PHGG samples from our institution’s pediatric brain tumor bank, we constructed a single-cell RNA-Seq (scRNA-Seq) dataset that included tumor and immune cells, generated predicted cell types by mapping the scRNA-Seq data onto known cell types, and performed differential gene expression and geneset enrichment analyses. We acquired bulk RNA-Seq and DNA methylation data to characterize overall tumor properties. RESULTS Samples consisted of several principal tumor cell types, including cells with astrocyte characteristics, oligodendrocyte progenitor cell (OPC) characteristics, and cells that expressed OPC markers but had slight enrichment in mesenchymal and inflammatory gene expression (OPC-like/Mes cells). We also found a potential stemlike population that expressed neural stem cell markers. The predicted astrocytes, OPCs and OPC-like/Mes cells differed in their gene expression profiles, pathway enrichment, and tendency to proliferate as assessed from expression of cell cycle genes. A combined microglia/macrophage population, strongly enriched in mesenchymal and inflammatory gene expression, may serve to induce inflammatory gene expression in tumor cells. Key conclusions from the scRNA-Seq analysis were validated using multi-channel immunofluorescence staining. CONCLUSIONS PHGG comprises a small stemlike population and varying proportions of proliferating glial lineage tumor cells. CNS-resident microglia/macrophages may trigger mesenchymal gene expression in PHGG. We will use our results to investigate the roles of PHGG’s diverse tumor cell populations in resistance to radiotherapy and to develop treatments to target individual cell types comprising PHGG." @default.
- W4380372983 created "2023-06-13" @default.
- W4380372983 creator A5008401630 @default.
- W4380372983 creator A5021375048 @default.
- W4380372983 creator A5022692329 @default.
- W4380372983 creator A5059042517 @default.
- W4380372983 creator A5060449174 @default.
- W4380372983 creator A5075671062 @default.
- W4380372983 creator A5080947810 @default.
- W4380372983 creator A5086095044 @default.
- W4380372983 date "2023-06-01" @default.
- W4380372983 modified "2023-09-23" @default.
- W4380372983 title "HGG-18. SINGLE CELL RNA-SEQ ANALYSIS OF PEDIATRIC HIGH-GRADE GLIOMA PATIENT SAMPLES IDENTIFIES COMMON GLIAL AND IMMUNE CELL TYPES THAT HELP TO EXPLAIN HETEROGENEITY AND TUMORIGENESIS" @default.
- W4380372983 doi "https://doi.org/10.1093/neuonc/noad073.167" @default.
- W4380372983 hasPublicationYear "2023" @default.
- W4380372983 type Work @default.
- W4380372983 citedByCount "0" @default.
- W4380372983 crossrefType "journal-article" @default.
- W4380372983 hasAuthorship W4380372983A5008401630 @default.
- W4380372983 hasAuthorship W4380372983A5021375048 @default.
- W4380372983 hasAuthorship W4380372983A5022692329 @default.
- W4380372983 hasAuthorship W4380372983A5059042517 @default.
- W4380372983 hasAuthorship W4380372983A5060449174 @default.
- W4380372983 hasAuthorship W4380372983A5075671062 @default.
- W4380372983 hasAuthorship W4380372983A5080947810 @default.
- W4380372983 hasAuthorship W4380372983A5086095044 @default.
- W4380372983 hasBestOaLocation W43803729831 @default.
- W4380372983 hasConcept C104317684 @default.
- W4380372983 hasConcept C136302470 @default.
- W4380372983 hasConcept C1491633281 @default.
- W4380372983 hasConcept C150194340 @default.
- W4380372983 hasConcept C162317418 @default.
- W4380372983 hasConcept C189014844 @default.
- W4380372983 hasConcept C190727270 @default.
- W4380372983 hasConcept C201750760 @default.
- W4380372983 hasConcept C203014093 @default.
- W4380372983 hasConcept C2776914184 @default.
- W4380372983 hasConcept C2776950831 @default.
- W4380372983 hasConcept C2778227246 @default.
- W4380372983 hasConcept C2779830541 @default.
- W4380372983 hasConcept C28328180 @default.
- W4380372983 hasConcept C2908647359 @default.
- W4380372983 hasConcept C502942594 @default.
- W4380372983 hasConcept C54355233 @default.
- W4380372983 hasConcept C555283112 @default.
- W4380372983 hasConcept C71924100 @default.
- W4380372983 hasConcept C86803240 @default.
- W4380372983 hasConcept C8891405 @default.
- W4380372983 hasConcept C99454951 @default.
- W4380372983 hasConceptScore W4380372983C104317684 @default.
- W4380372983 hasConceptScore W4380372983C136302470 @default.
- W4380372983 hasConceptScore W4380372983C1491633281 @default.
- W4380372983 hasConceptScore W4380372983C150194340 @default.
- W4380372983 hasConceptScore W4380372983C162317418 @default.
- W4380372983 hasConceptScore W4380372983C189014844 @default.
- W4380372983 hasConceptScore W4380372983C190727270 @default.
- W4380372983 hasConceptScore W4380372983C201750760 @default.
- W4380372983 hasConceptScore W4380372983C203014093 @default.
- W4380372983 hasConceptScore W4380372983C2776914184 @default.
- W4380372983 hasConceptScore W4380372983C2776950831 @default.
- W4380372983 hasConceptScore W4380372983C2778227246 @default.
- W4380372983 hasConceptScore W4380372983C2779830541 @default.
- W4380372983 hasConceptScore W4380372983C28328180 @default.
- W4380372983 hasConceptScore W4380372983C2908647359 @default.
- W4380372983 hasConceptScore W4380372983C502942594 @default.
- W4380372983 hasConceptScore W4380372983C54355233 @default.
- W4380372983 hasConceptScore W4380372983C555283112 @default.
- W4380372983 hasConceptScore W4380372983C71924100 @default.
- W4380372983 hasConceptScore W4380372983C86803240 @default.
- W4380372983 hasConceptScore W4380372983C8891405 @default.
- W4380372983 hasConceptScore W4380372983C99454951 @default.
- W4380372983 hasIssue "Supplement_1" @default.
- W4380372983 hasLocation W43803729831 @default.
- W4380372983 hasLocation W43803729832 @default.
- W4380372983 hasOpenAccess W4380372983 @default.
- W4380372983 hasPrimaryLocation W43803729831 @default.
- W4380372983 hasRelatedWork W133445481 @default.
- W4380372983 hasRelatedWork W1976203623 @default.
- W4380372983 hasRelatedWork W2003499218 @default.
- W4380372983 hasRelatedWork W2258579332 @default.
- W4380372983 hasRelatedWork W2513916811 @default.
- W4380372983 hasRelatedWork W2523620612 @default.
- W4380372983 hasRelatedWork W2998722864 @default.
- W4380372983 hasRelatedWork W3046385446 @default.
- W4380372983 hasRelatedWork W3093470024 @default.
- W4380372983 hasRelatedWork W4283385293 @default.
- W4380372983 hasVolume "25" @default.
- W4380372983 isParatext "false" @default.
- W4380372983 isRetracted "false" @default.
- W4380372983 workType "article" @default.