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• W4380450050 abstract "Abstract Reduced genome bacteria are genetically simplified systems that facilitate biological study and industrial use. The free-living Alphaproteobacterium, Zymomonas mobilis , has a naturally reduced genome containing fewer than 2000 protein coding genes. Despite its small genome, Z. mobilis thrives in diverse conditions including the presence or absence of atmospheric oxygen. However, insufficient characterization of essential and conditionally essential genes has limited broader adoption of Z. mobilis as a model Alphaproteobacterium. Here, we use genome-scale CRISPRi-seq to systematically identify and characterize Z. mobilis genes that are conditionally essential for aerotolerant or anaerobic growth, or are generally essential across both conditions. Comparative genomics revealed that the essentiality of most “generally essential” genes was shared between Z. mobilis and other Alphaproteobacteria, validating Z. mobilis as reduced genome model. Among conditionally essential genes, we found that the DNA repair gene, recJ, was critical only for aerobic growth but reduced the mutation rate under both conditions. Further, we show that genes encoding the F 1 F O ATP synthase and Rnf respiratory complex are required for anaerobic growth of Z. mobilis . Combining CRISPRi partial knockdowns with metabolomics and membrane potential measurements, we determined that the ATP synthase generates membrane potential that is consumed by Rnf to power downstream processes. Rnf knockdown strains accumulated isoprenoid biosynthesis intermediates, suggesting a key role for Rnf in powering essential biosynthetic reactions. Our work establishes Z. mobilis as a streamlined model for alphaproteobacterial genetics, has broad implications in bacterial energy coupling, and informs Z. mobilis genome manipulation for optimized production of valuable isoprenoid-based bioproducts. Importance The inherent complexity of biological systems is a major barrier to our understanding of cellular physiology. Bacteria with markedly fewer genes than their close relatives, or reduced genome bacteria, are promising biological models with less complexity. Reduced genome bacteria can also have superior properties for industrial use, provided the reduction does not overly restrict strain robustness. Naturally reduced genome bacteria, such as the Alphaproteobacterium, Zymomonas mobilis , have fewer genes but remain environmentally robust. In this study, we show that Z. mobilis is a simplified genetic model for Alphaproteobacteria, a class with important impacts on the environment, human health, and industry. We also identify genes that are only required in the absence of atmospheric oxygen, uncovering players that maintain and utilize the cellular energy state. Our findings have broad implications for the genetics of Alphaproteobacteria and industrial use of Z. mobilis to create biofuels and bioproducts." @default.
• W4380450050 created "2023-06-14" @default.
• W4380450050 creator A5000329370 @default.
• W4380450050 creator A5012990763 @default.
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• W4380450050 creator A5063353715 @default.
• W4380450050 creator A5073073926 @default.
• W4380450050 date "2023-06-13" @default.
• W4380450050 modified "2023-09-24" @default.
• W4380450050 title "The Genetics of Aerotolerant Growth in a Naturally Reduced Genome Alphaproteobacterium" @default.
• W4380450050 cites W140547030 @default.
• W4380450050 cites W1557050311 @default.
• W4380450050 cites W1679353543 @default.
• W4380450050 cites W1835408291 @default.
• W4380450050 cites W1847092923 @default.
• W4380450050 cites W1900937478 @default.
• W4380450050 cites W1901295806 @default.
• W4380450050 cites W1907277512 @default.
• W4380450050 cites W1932877617 @default.
• W4380450050 cites W1965893940 @default.
• W4380450050 cites W1966749659 @default.
• W4380450050 cites W1972993849 @default.
• W4380450050 cites W1978790450 @default.
• W4380450050 cites W1980017270 @default.
• W4380450050 cites W1980021255 @default.
• W4380450050 cites W1985657568 @default.
• W4380450050 cites W1997338312 @default.
• W4380450050 cites W1999712993 @default.
• W4380450050 cites W2005447726 @default.
• W4380450050 cites W2009580263 @default.
• W4380450050 cites W2011976040 @default.
• W4380450050 cites W2030924818 @default.
• W4380450050 cites W2034558349 @default.
• W4380450050 cites W2036897871 @default.
• W4380450050 cites W2052495667 @default.
• W4380450050 cites W2060095084 @default.
• W4380450050 cites W2063670078 @default.
• W4380450050 cites W2064671512 @default.
• W4380450050 cites W2097002638 @default.
• W4380450050 cites W2103727397 @default.
• W4380450050 cites W2107764928 @default.
• W4380450050 cites W2108385415 @default.
• W4380450050 cites W2111967267 @default.
• W4380450050 cites W2119025443 @default.
• W4380450050 cites W2124469739 @default.
• W4380450050 cites W2131271579 @default.
• W4380450050 cites W2136984776 @default.
• W4380450050 cites W2147469143 @default.
• W4380450050 cites W2152364643 @default.
• W4380450050 cites W2152856606 @default.
• W4380450050 cites W2154598603 @default.
• W4380450050 cites W2155608359 @default.
• W4380450050 cites W2159916488 @default.
• W4380450050 cites W2160051211 @default.
• W4380450050 cites W2172228379 @default.
• W4380450050 cites W2267069928 @default.
• W4380450050 cites W2407174002 @default.
• W4380450050 cites W2551836638 @default.
• W4380450050 cites W2586340362 @default.
• W4380450050 cites W2586637020 @default.
• W4380450050 cites W2594247767 @default.
• W4380450050 cites W2621469050 @default.
• W4380450050 cites W2804515387 @default.
• W4380450050 cites W2804657653 @default.
• W4380450050 cites W2807249764 @default.
• W4380450050 cites W2906110636 @default.
• W4380450050 cites W2913704529 @default.
• W4380450050 cites W2922061275 @default.
• W4380450050 cites W2927873716 @default.
• W4380450050 cites W2944003288 @default.
• W4380450050 cites W2944543437 @default.
• W4380450050 cites W2953131670 @default.
• W4380450050 cites W2967192906 @default.
• W4380450050 cites W2970503599 @default.
• W4380450050 cites W2985649852 @default.
• W4380450050 cites W2988396470 @default.
• W4380450050 cites W2995080222 @default.
• W4380450050 cites W3002976940 @default.
• W4380450050 cites W3039221959 @default.
• W4380450050 cites W3045162400 @default.
• W4380450050 cites W3046424383 @default.
• W4380450050 cites W3050368877 @default.
• W4380450050 cites W3088639284 @default.
• W4380450050 cites W3093163365 @default.
• W4380450050 cites W3103145119 @default.
• W4380450050 cites W3148105786 @default.
• W4380450050 cites W3159315578 @default.
• W4380450050 cites W3176233387 @default.
• W4380450050 cites W3188250587 @default.
• W4380450050 cites W3216903231 @default.
• W4380450050 cites W4200416587 @default.
• W4380450050 cites W4206724403 @default.
• W4380450050 cites W4226271116 @default.
• W4380450050 cites W4293792660 @default.
• W4380450050 cites W4303633806 @default.
• W4380450050 cites W4304845502 @default.

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