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- W4380487030 abstract "Abstract Nonlinear microscopy, including two-photon microscopy, requires pulsed lasers as light source. Typically, when choosing the appropriate pulsed laser for two-photon microscopy, the pulse repetition rate, pulse width, total power output, and output beam diameter are among the critical parameters which are often emphasised. Here, we demonstrate that the pulse shape, often overlooked, can have significant impact on the two-photon microscopy excitation efficiency and the effective signal brightness. We provide metrics to ease practical selection of pulsed laser sources for two-photon microscopy." @default.
- W4380487030 created "2023-06-14" @default.
- W4380487030 creator A5037379072 @default.
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- W4380487030 date "2023-06-13" @default.
- W4380487030 modified "2023-09-27" @default.
- W4380487030 title "The influence of laser pulse shape and cleanliness on two-photon microscopy" @default.
- W4380487030 doi "https://doi.org/10.21203/rs.3.rs-3019234/v1" @default.
- W4380487030 hasPublicationYear "2023" @default.
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