FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4380488907> ?p ?o ?g. }

• W4380488907 abstract "Abstract Background. Severe asthma (SA) is a heterogeneous condition with multiple phenotypes. There is still an unmet need to characterize and understand underlying mechanisms taking place in the lungs in order to propose the most suitable therapeutic strategies for SA. For this purpose, we aimed to identify a local signature of severe asthma by conducting comprehensive multi-omics analysis of bronchoalveolar lavages fluids (BALs) from children with SA versus non-asthmatic (NA) controls. Method. BALs were collected from twenty children with SA and from ten age-matched NA. We previously analyzed soluble and cellular immune components in those samples, and now propose to perform comprehensive analysis of their microbiota and their metabolome. Briefly, DNA from BALs was extracted and 16S rRNA gene (V3-V4 region) was amplified by PCR and sequenced. In parallel, untargeted metabolomics was performed using liquid chromatography coupled to high resolution mass spectrometry (LC-HRMS) following an established workflow for sample preparation, data acquisition and treatment. Each microbiome and metabolome dataset was first analysed independently by unsupervised multivariate analyses (Principal component analyses, PCA). Differences between groups for microbiota diversity indices, the relative distribution of each phyla and genera were then analysed. Metabolite set enrichment analysis (MSEA) and univariate supervised analysis were also performed. To identify a local signature of severe asthma, microbiota and metabolome data were further integrated, together with immune and with clinical data, using unsupervised Multi-Omics Factor Analysis (MOFA). Results. Microbiota diversity was higher in children with SA versus NA, with higher relative abundances of Streptococcus , Corynebacterium , Tropheryma whipplei , Dolosigranulum pigrum and Moraxella nonliquefaciens . We identified 88 metabolites in BALs, but unsupervised PCA of corresponding data did not differentiate children with SA from NA. However, MSEA evidenced that biotin and carnitine synthesis, lysine degradation, methionine metabolism and spermidine and spermine biosynthesis pathways were significantly enriched in children with SA. Finally, multiblocks data integration identified a signature of SA, mainly described by metabolites and cytokines. Conclusion. By integrating metabolome, microbiome and cytokines data obtained on BALs from children with severe asthma versus NA, our study uniquely described a local signature of SA." @default.
• W4380488907 created "2023-06-14" @default.
• W4380488907 creator A5026848147 @default.
• W4380488907 creator A5028205376 @default.
• W4380488907 creator A5028223956 @default.
• W4380488907 creator A5030832468 @default.
• W4380488907 creator A5033514706 @default.
• W4380488907 creator A5043918637 @default.
• W4380488907 creator A5061353015 @default.
• W4380488907 creator A5063709998 @default.
• W4380488907 creator A5085377597 @default.
• W4380488907 creator A5085964029 @default.
• W4380488907 creator A5089191136 @default.
• W4380488907 creator A5090278719 @default.
• W4380488907 creator A5091787800 @default.
• W4380488907 date "2023-06-13" @default.
• W4380488907 modified "2023-09-30" @default.
• W4380488907 title "Cytokines and metabolites are the main descriptors of severe asthma in children when performing multi-omics analysis of bronchoaleveolar lavages" @default.
• W4380488907 cites W1542886040 @default.
• W4380488907 cites W1611854178 @default.
• W4380488907 cites W1916333704 @default.
• W4380488907 cites W1964010911 @default.
• W4380488907 cites W2002129459 @default.
• W4380488907 cites W2020848465 @default.
• W4380488907 cites W2044091012 @default.
• W4380488907 cites W2050591900 @default.
• W4380488907 cites W2066854584 @default.
• W4380488907 cites W2085945130 @default.
• W4380488907 cites W2093488444 @default.
• W4380488907 cites W2098276532 @default.
• W4380488907 cites W2112594020 @default.
• W4380488907 cites W2118508632 @default.
• W4380488907 cites W2119016887 @default.
• W4380488907 cites W2157688534 @default.
• W4380488907 cites W2292907486 @default.
• W4380488907 cites W2341820027 @default.
• W4380488907 cites W2432815617 @default.
• W4380488907 cites W2563030504 @default.
• W4380488907 cites W2603590667 @default.
• W4380488907 cites W2611050494 @default.
• W4380488907 cites W2755244027 @default.
• W4380488907 cites W2768234824 @default.
• W4380488907 cites W2774551105 @default.
• W4380488907 cites W2800636439 @default.
• W4380488907 cites W2804360535 @default.
• W4380488907 cites W2808845633 @default.
• W4380488907 cites W2888258413 @default.
• W4380488907 cites W2892016825 @default.
• W4380488907 cites W2904339023 @default.
• W4380488907 cites W2910142318 @default.
• W4380488907 cites W2953371836 @default.
• W4380488907 cites W2968853464 @default.
• W4380488907 cites W2977742032 @default.
• W4380488907 cites W2995154691 @default.
• W4380488907 cites W3008278285 @default.
• W4380488907 cites W3021422742 @default.
• W4380488907 cites W3027051073 @default.
• W4380488907 cites W3111022186 @default.
• W4380488907 cites W3126733293 @default.
• W4380488907 cites W3134582970 @default.
• W4380488907 cites W3183444860 @default.
• W4380488907 cites W3193659472 @default.
• W4380488907 cites W3205256661 @default.
• W4380488907 cites W3216386447 @default.
• W4380488907 cites W4229008231 @default.
• W4380488907 cites W4283017342 @default.
• W4380488907 cites W4283118990 @default.
• W4380488907 cites W4292738137 @default.
• W4380488907 cites W4309474629 @default.
• W4380488907 cites W4312082462 @default.
• W4380488907 cites W4324377997 @default.
• W4380488907 doi "https://doi.org/10.21203/rs.3.rs-3034067/v1" @default.
• W4380488907 hasPublicationYear "2023" @default.
• W4380488907 type Work @default.
• W4380488907 citedByCount "0" @default.
• W4380488907 crossrefType "posted-content" @default.
• W4380488907 hasAuthorship W4380488907A5026848147 @default.
• W4380488907 hasAuthorship W4380488907A5028205376 @default.
• W4380488907 hasAuthorship W4380488907A5028223956 @default.
• W4380488907 hasAuthorship W4380488907A5030832468 @default.
• W4380488907 hasAuthorship W4380488907A5033514706 @default.
• W4380488907 hasAuthorship W4380488907A5043918637 @default.
• W4380488907 hasAuthorship W4380488907A5061353015 @default.
• W4380488907 hasAuthorship W4380488907A5063709998 @default.
• W4380488907 hasAuthorship W4380488907A5085377597 @default.
• W4380488907 hasAuthorship W4380488907A5085964029 @default.
• W4380488907 hasAuthorship W4380488907A5089191136 @default.
• W4380488907 hasAuthorship W4380488907A5090278719 @default.
• W4380488907 hasAuthorship W4380488907A5091787800 @default.
• W4380488907 hasBestOaLocation W43804889071 @default.
• W4380488907 hasConcept C104317684 @default.
• W4380488907 hasConcept C135870905 @default.
• W4380488907 hasConcept C143121216 @default.
• W4380488907 hasConcept C150194340 @default.
• W4380488907 hasConcept C157585117 @default.
• W4380488907 hasConcept C162317418 @default.
• W4380488907 hasConcept C21565614 @default.
• W4380488907 hasConcept C54355233 @default.
• W4380488907 hasConcept C60644358 @default.
• W4380488907 hasConcept C70721500 @default.

• next