FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4380624226> ?p ?o ?g. }

• W4380624226 abstract "Abstract Overexpression of the multidrug efflux pump MtrCDE, a critical factor of multidrug-resistance in Neisseria gonorrhoeae , the causative agent of gonorrheae, is repressed by the transcriptional regulator, MtrR (multiple transferable resistance repressor). Here, we report the results from a series of in vitro experiments to identify innate, human inducers of MtrR and to understand the biochemical and structural mechanisms of the gene regulatory function of MtrR. Isothermal titration calorimetry experiments reveal that MtrR binds the hormonal steroids progesterone, β-estradiol, and testosterone, all of which are present at significant concentrations at urogenital infection sites as well as ethinyl estrogen, a component of some birth control pills. Binding of these steroids results in decreased affinity of MtrR for cognate DNA, as demonstrated by fluorescence polarization-based assays. The crystal structures of MtrR bound to each steroid provided insight into the flexibility of the binding pocket, elucidated specific residue-ligand interactions, and revealed the conformational consequences of the induction mechanism of MtrR. Three residues, D171, W136 and R176 are key to the specific binding of these gonadal steroids. These studies provide a molecular understanding of the transcriptional regulation by MtrR that promotes N. gonorrhoeae survival in its human host." @default.
• W4380624226 created "2023-06-15" @default.
• W4380624226 creator A5000654851 @default.
• W4380624226 creator A5018104424 @default.
• W4380624226 creator A5028777325 @default.
• W4380624226 creator A5032576498 @default.
• W4380624226 creator A5045847541 @default.
• W4380624226 creator A5060132420 @default.
• W4380624226 creator A5090930856 @default.
• W4380624226 date "2023-06-13" @default.
• W4380624226 modified "2023-09-26" @default.
• W4380624226 title "Hormonal steroids bind the<i>Neisseria gonorrhoeae</i>multidrug resistance regulator, MtrR, to induce a multidrug binding efflux pump and stress-response sigma factor" @default.
• W4380624226 cites W1726597626 @default.
• W4380624226 cites W1816644759 @default.
• W4380624226 cites W1967593111 @default.
• W4380624226 cites W1990248547 @default.
• W4380624226 cites W1993063963 @default.
• W4380624226 cites W2015524479 @default.
• W4380624226 cites W2026624424 @default.
• W4380624226 cites W2052354811 @default.
• W4380624226 cites W2069805306 @default.
• W4380624226 cites W2082295788 @default.
• W4380624226 cites W2084045864 @default.
• W4380624226 cites W2112586763 @default.
• W4380624226 cites W2113556104 @default.
• W4380624226 cites W2128068892 @default.
• W4380624226 cites W2131590438 @default.
• W4380624226 cites W2134809564 @default.
• W4380624226 cites W2136723682 @default.
• W4380624226 cites W2144081223 @default.
• W4380624226 cites W2146955039 @default.
• W4380624226 cites W2149433111 @default.
• W4380624226 cites W2154197653 @default.
• W4380624226 cites W2160043086 @default.
• W4380624226 cites W2160592803 @default.
• W4380624226 cites W2167722481 @default.
• W4380624226 cites W2170700961 @default.
• W4380624226 cites W2180229411 @default.
• W4380624226 cites W2529034339 @default.
• W4380624226 cites W2584091912 @default.
• W4380624226 cites W2725319213 @default.
• W4380624226 cites W2765322245 @default.
• W4380624226 cites W2789808681 @default.
• W4380624226 cites W2913786501 @default.
• W4380624226 cites W2944930475 @default.
• W4380624226 cites W2963541510 @default.
• W4380624226 cites W2963585748 @default.
• W4380624226 cites W2976885709 @default.
• W4380624226 cites W2985995347 @default.
• W4380624226 cites W3147870042 @default.
• W4380624226 cites W4210955077 @default.
• W4380624226 doi "https://doi.org/10.1101/2023.06.13.544409" @default.
• W4380624226 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37398116" @default.
• W4380624226 hasPublicationYear "2023" @default.
• W4380624226 type Work @default.
• W4380624226 citedByCount "0" @default.
• W4380624226 crossrefType "posted-content" @default.
• W4380624226 hasAuthorship W4380624226A5000654851 @default.
• W4380624226 hasAuthorship W4380624226A5018104424 @default.
• W4380624226 hasAuthorship W4380624226A5028777325 @default.
• W4380624226 hasAuthorship W4380624226A5032576498 @default.
• W4380624226 hasAuthorship W4380624226A5045847541 @default.
• W4380624226 hasAuthorship W4380624226A5060132420 @default.
• W4380624226 hasAuthorship W4380624226A5090930856 @default.
• W4380624226 hasBestOaLocation W43806242261 @default.
• W4380624226 hasConcept C101762097 @default.
• W4380624226 hasConcept C104317684 @default.
• W4380624226 hasConcept C135763542 @default.
• W4380624226 hasConcept C150194340 @default.
• W4380624226 hasConcept C158448853 @default.
• W4380624226 hasConcept C2778087339 @default.
• W4380624226 hasConcept C2779166590 @default.
• W4380624226 hasConcept C43563269 @default.
• W4380624226 hasConcept C47319516 @default.
• W4380624226 hasConcept C54355233 @default.
• W4380624226 hasConcept C86339819 @default.
• W4380624226 hasConcept C86803240 @default.
• W4380624226 hasConceptScore W4380624226C101762097 @default.
• W4380624226 hasConceptScore W4380624226C104317684 @default.
• W4380624226 hasConceptScore W4380624226C135763542 @default.
• W4380624226 hasConceptScore W4380624226C150194340 @default.
• W4380624226 hasConceptScore W4380624226C158448853 @default.
• W4380624226 hasConceptScore W4380624226C2778087339 @default.
• W4380624226 hasConceptScore W4380624226C2779166590 @default.
• W4380624226 hasConceptScore W4380624226C43563269 @default.
• W4380624226 hasConceptScore W4380624226C47319516 @default.
• W4380624226 hasConceptScore W4380624226C54355233 @default.
• W4380624226 hasConceptScore W4380624226C86339819 @default.
• W4380624226 hasConceptScore W4380624226C86803240 @default.
• W4380624226 hasLocation W43806242261 @default.
• W4380624226 hasLocation W43806242262 @default.
• W4380624226 hasLocation W43806242263 @default.
• W4380624226 hasOpenAccess W4380624226 @default.
• W4380624226 hasPrimaryLocation W43806242261 @default.
• W4380624226 hasRelatedWork W1601030124 @default.
• W4380624226 hasRelatedWork W1987587087 @default.
• W4380624226 hasRelatedWork W1988972551 @default.
• W4380624226 hasRelatedWork W2053434099 @default.
• W4380624226 hasRelatedWork W2109489214 @default.
• W4380624226 hasRelatedWork W2145310171 @default.

• next