FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4380768427> ?p ?o ?g. }

• W4380768427 endingPage "e39" @default.
• W4380768427 startingPage "e39" @default.
• W4380768427 abstract "Objective Cancer stem cells (CSC) comprise a subpopulation of tumor cells associated with initiation, progression, and chemoresistance. Subpopulations of CSC were characterized in a metastatic cell line LN1-A derived from the oral squamous cell carcinoma (OSCC), previously isolated in our laboratory. Study Design We selected three subpopulations of CSC (CD44Low/CD326+, CD44+/CD326-, and CD44High/CD326-) among the seven phenotypes isolated by labeling CD44 and CD326 antibodies by flow cytometry in metastatic LN-1A cells. Cell morphology, proliferation, migration, adhesion, clonogenic assay, cell cycle, cell death, and cisplatin chemoresistance were characterized. Western blotting for EFGR, FASN, p-AKT, CPT1-α, and mesenchymal epithelium transition markers were performed. Results CD44Low/CD326+ subpopulations showed an epithelial phenotype with polygonal morphology and high E-cadherin expression, while CD44+/CD326- and CD44High/CD326- showed fusiform morphology with high Vimentin and Slug expressions, suggesting a mesenchymal phenotype. Proliferation was higher in CD44+/CD326- and CD44High/CD326- cells, which was accompanied by increased levels of p-AKT. Cell adhesion and colony formation were increased in CD44+/CD326- and CD44High/CD326-, while cell death was higher in CD44Low/CD326+. No significant differences in cell cycle, cisplatin resistance, and the expression of EGFR, FASN, and, CPT1-α were observed between phenotypes. Conclusion Our results showed that CD44+/CD326- and CD44High/CD326- cells show a more aggressive phenotype. Cancer stem cells (CSC) comprise a subpopulation of tumor cells associated with initiation, progression, and chemoresistance. Subpopulations of CSC were characterized in a metastatic cell line LN1-A derived from the oral squamous cell carcinoma (OSCC), previously isolated in our laboratory. We selected three subpopulations of CSC (CD44Low/CD326+, CD44+/CD326-, and CD44High/CD326-) among the seven phenotypes isolated by labeling CD44 and CD326 antibodies by flow cytometry in metastatic LN-1A cells. Cell morphology, proliferation, migration, adhesion, clonogenic assay, cell cycle, cell death, and cisplatin chemoresistance were characterized. Western blotting for EFGR, FASN, p-AKT, CPT1-α, and mesenchymal epithelium transition markers were performed. CD44Low/CD326+ subpopulations showed an epithelial phenotype with polygonal morphology and high E-cadherin expression, while CD44+/CD326- and CD44High/CD326- showed fusiform morphology with high Vimentin and Slug expressions, suggesting a mesenchymal phenotype. Proliferation was higher in CD44+/CD326- and CD44High/CD326- cells, which was accompanied by increased levels of p-AKT. Cell adhesion and colony formation were increased in CD44+/CD326- and CD44High/CD326-, while cell death was higher in CD44Low/CD326+. No significant differences in cell cycle, cisplatin resistance, and the expression of EGFR, FASN, and, CPT1-α were observed between phenotypes. Our results showed that CD44+/CD326- and CD44High/CD326- cells show a more aggressive phenotype." @default.
• W4380768427 created "2023-06-16" @default.
• W4380768427 creator A5002941949 @default.
• W4380768427 creator A5003106349 @default.
• W4380768427 creator A5014037521 @default.
• W4380768427 creator A5029367134 @default.
• W4380768427 creator A5029777124 @default.
• W4380768427 creator A5029843488 @default.
• W4380768427 creator A5078348511 @default.
• W4380768427 date "2023-07-01" @default.
• W4380768427 modified "2023-09-27" @default.
• W4380768427 title "CANCER STEM CELLS IN ORAL CANCER: PHENOTYPIC COMPARISON BETWEEN SUBPOPULATIONS LABELED WITH CD44 AND CD326 ANTIBODIES." @default.
• W4380768427 doi "https://doi.org/10.1016/j.oooo.2023.03.097" @default.
• W4380768427 hasPublicationYear "2023" @default.
• W4380768427 type Work @default.
• W4380768427 citedByCount "0" @default.
• W4380768427 crossrefType "journal-article" @default.
• W4380768427 hasAuthorship W4380768427A5002941949 @default.
• W4380768427 hasAuthorship W4380768427A5003106349 @default.
• W4380768427 hasAuthorship W4380768427A5014037521 @default.
• W4380768427 hasAuthorship W4380768427A5029367134 @default.
• W4380768427 hasAuthorship W4380768427A5029777124 @default.
• W4380768427 hasAuthorship W4380768427A5029843488 @default.
• W4380768427 hasAuthorship W4380768427A5078348511 @default.
• W4380768427 hasConcept C121608353 @default.
• W4380768427 hasConcept C126322002 @default.
• W4380768427 hasConcept C147447768 @default.
• W4380768427 hasConcept C1491633281 @default.
• W4380768427 hasConcept C203014093 @default.
• W4380768427 hasConcept C204232928 @default.
• W4380768427 hasConcept C2780932548 @default.
• W4380768427 hasConcept C28328180 @default.
• W4380768427 hasConcept C502942594 @default.
• W4380768427 hasConcept C54355233 @default.
• W4380768427 hasConcept C553184892 @default.
• W4380768427 hasConcept C55427017 @default.
• W4380768427 hasConcept C71924100 @default.
• W4380768427 hasConcept C86803240 @default.
• W4380768427 hasConcept C95444343 @default.
• W4380768427 hasConcept C96232424 @default.
• W4380768427 hasConceptScore W4380768427C121608353 @default.
• W4380768427 hasConceptScore W4380768427C126322002 @default.
• W4380768427 hasConceptScore W4380768427C147447768 @default.
• W4380768427 hasConceptScore W4380768427C1491633281 @default.
• W4380768427 hasConceptScore W4380768427C203014093 @default.
• W4380768427 hasConceptScore W4380768427C204232928 @default.
• W4380768427 hasConceptScore W4380768427C2780932548 @default.
• W4380768427 hasConceptScore W4380768427C28328180 @default.
• W4380768427 hasConceptScore W4380768427C502942594 @default.
• W4380768427 hasConceptScore W4380768427C54355233 @default.
• W4380768427 hasConceptScore W4380768427C553184892 @default.
• W4380768427 hasConceptScore W4380768427C55427017 @default.
• W4380768427 hasConceptScore W4380768427C71924100 @default.
• W4380768427 hasConceptScore W4380768427C86803240 @default.
• W4380768427 hasConceptScore W4380768427C95444343 @default.
• W4380768427 hasConceptScore W4380768427C96232424 @default.
• W4380768427 hasIssue "1" @default.
• W4380768427 hasLocation W43807684271 @default.
• W4380768427 hasOpenAccess W4380768427 @default.
• W4380768427 hasPrimaryLocation W43807684271 @default.
• W4380768427 hasRelatedWork W1986030763 @default.
• W4380768427 hasRelatedWork W2013148168 @default.
• W4380768427 hasRelatedWork W2158955249 @default.
• W4380768427 hasRelatedWork W2161492309 @default.
• W4380768427 hasRelatedWork W2341694940 @default.
• W4380768427 hasRelatedWork W2343191660 @default.
• W4380768427 hasRelatedWork W2363859992 @default.
• W4380768427 hasRelatedWork W2601778356 @default.
• W4380768427 hasRelatedWork W2741545625 @default.
• W4380768427 hasRelatedWork W3151381940 @default.
• W4380768427 hasVolume "136" @default.
• W4380768427 isParatext "false" @default.
• W4380768427 isRetracted "false" @default.
• W4380768427 workType "article" @default.