Matches in SemOpenAlex for { <https://semopenalex.org/work/W4380794908> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W4380794908 abstract "Introduction: Endothelial cell (EC) permeability is essential to vascular homeostasis in diabetes. MicroRNAs (miRs) are critical gene regulators whose roles in the EC permeability have yet to be elucidated. It remains unclear whether miR-200 and miR-466 regulate EC permeability. We hypothesized that the inhibition of miR-466 and miR-200 can restore vascular integrity and accelerate wound healing via targeting Claudin-5. Methods and Results: Human dermal microvascular ECs from healthy subjects and type 2 diabetic patients at passages 5-7 were cultured in vitro. The EC permeability was significantly increased by high glucose (25mM)or silencing Claudin-5 as measured by FITC-Dextran Transwell assay (n = 5, p<0.05). Claudin-5 protein levels were significantly reduced in diabetic ECs and healthy ECs exposed to high glucose. The reduction of Claudin-5 protein was restored by the inhibition of miR-466 (Western Blot, n = 5, p<0.05), though both miR-200 and miR-466 are directly bound with the 3’ UTR of Claudin-5 mRNA (luciferase 3’UTR reporter assay). Furthermore, silencing Claudin-5 diminished the protection of miR-200/miR-466 inhibition on EC permeability damaged by high glucose (n = 5, p<0.05). In the in vivo study, the topical application of miR-200 and miR-466 inhibitors showed enhanced efficacy in accelerating the closure of a 7-mm full-thickness excisional wound in a type 2 diabetic (db/db) mouse model, with concomitant robust wound capillary formation (CD31 immunochemistry), compared with the topical application of miR-200 inhibitor alone (n = 5, p<0.05). Claudin-5 levels were increased in the wounds receiving miR-466 inhibitor alone or both miR-200 and miR-466 inhibitors (n = 5, p<0.05). Conclusions: Our study demonstrated the potentially effective approach of miR-200/miR-466 cocktail inhibition to restore vascular integrity and tissue repair in hyperglycemia. This may provide a novel therapeutic strategy targeting miRs for the treatment of wound healing in diabetes." @default.
- W4380794908 created "2023-06-16" @default.
- W4380794908 creator A5050465187 @default.
- W4380794908 creator A5051416943 @default.
- W4380794908 creator A5052005789 @default.
- W4380794908 creator A5070994650 @default.
- W4380794908 creator A5085555534 @default.
- W4380794908 date "2022-11-08" @default.
- W4380794908 modified "2023-09-24" @default.
- W4380794908 title "Abstract 13182: Inhibition of Microrna-466 and Microrna-200 Accelerates Wound Healing by Decreasing Endothelial Cell Permeability" @default.
- W4380794908 doi "https://doi.org/10.1161/circ.146.suppl_1.13182" @default.
- W4380794908 hasPublicationYear "2022" @default.
- W4380794908 type Work @default.
- W4380794908 citedByCount "0" @default.
- W4380794908 crossrefType "journal-article" @default.
- W4380794908 hasAuthorship W4380794908A5050465187 @default.
- W4380794908 hasAuthorship W4380794908A5051416943 @default.
- W4380794908 hasAuthorship W4380794908A5052005789 @default.
- W4380794908 hasAuthorship W4380794908A5070994650 @default.
- W4380794908 hasAuthorship W4380794908A5085555534 @default.
- W4380794908 hasConcept C104317684 @default.
- W4380794908 hasConcept C113045295 @default.
- W4380794908 hasConcept C119056186 @default.
- W4380794908 hasConcept C123012128 @default.
- W4380794908 hasConcept C127561419 @default.
- W4380794908 hasConcept C134018914 @default.
- W4380794908 hasConcept C145059251 @default.
- W4380794908 hasConcept C150903083 @default.
- W4380794908 hasConcept C153911025 @default.
- W4380794908 hasConcept C177779419 @default.
- W4380794908 hasConcept C202751555 @default.
- W4380794908 hasConcept C203014093 @default.
- W4380794908 hasConcept C207001950 @default.
- W4380794908 hasConcept C2776415932 @default.
- W4380794908 hasConcept C2778939058 @default.
- W4380794908 hasConcept C2780269544 @default.
- W4380794908 hasConcept C2780394083 @default.
- W4380794908 hasConcept C502942594 @default.
- W4380794908 hasConcept C51872919 @default.
- W4380794908 hasConcept C55493867 @default.
- W4380794908 hasConcept C71924100 @default.
- W4380794908 hasConcept C86803240 @default.
- W4380794908 hasConcept C95444343 @default.
- W4380794908 hasConcept C98274493 @default.
- W4380794908 hasConceptScore W4380794908C104317684 @default.
- W4380794908 hasConceptScore W4380794908C113045295 @default.
- W4380794908 hasConceptScore W4380794908C119056186 @default.
- W4380794908 hasConceptScore W4380794908C123012128 @default.
- W4380794908 hasConceptScore W4380794908C127561419 @default.
- W4380794908 hasConceptScore W4380794908C134018914 @default.
- W4380794908 hasConceptScore W4380794908C145059251 @default.
- W4380794908 hasConceptScore W4380794908C150903083 @default.
- W4380794908 hasConceptScore W4380794908C153911025 @default.
- W4380794908 hasConceptScore W4380794908C177779419 @default.
- W4380794908 hasConceptScore W4380794908C202751555 @default.
- W4380794908 hasConceptScore W4380794908C203014093 @default.
- W4380794908 hasConceptScore W4380794908C207001950 @default.
- W4380794908 hasConceptScore W4380794908C2776415932 @default.
- W4380794908 hasConceptScore W4380794908C2778939058 @default.
- W4380794908 hasConceptScore W4380794908C2780269544 @default.
- W4380794908 hasConceptScore W4380794908C2780394083 @default.
- W4380794908 hasConceptScore W4380794908C502942594 @default.
- W4380794908 hasConceptScore W4380794908C51872919 @default.
- W4380794908 hasConceptScore W4380794908C55493867 @default.
- W4380794908 hasConceptScore W4380794908C71924100 @default.
- W4380794908 hasConceptScore W4380794908C86803240 @default.
- W4380794908 hasConceptScore W4380794908C95444343 @default.
- W4380794908 hasConceptScore W4380794908C98274493 @default.
- W4380794908 hasIssue "Suppl_1" @default.
- W4380794908 hasLocation W43807949081 @default.
- W4380794908 hasOpenAccess W4380794908 @default.
- W4380794908 hasPrimaryLocation W43807949081 @default.
- W4380794908 hasRelatedWork W2052926478 @default.
- W4380794908 hasRelatedWork W2171767433 @default.
- W4380794908 hasRelatedWork W2317982666 @default.
- W4380794908 hasRelatedWork W2415734547 @default.
- W4380794908 hasRelatedWork W2956142642 @default.
- W4380794908 hasRelatedWork W3154489017 @default.
- W4380794908 hasRelatedWork W4283832979 @default.
- W4380794908 hasRelatedWork W4380794908 @default.
- W4380794908 hasRelatedWork W1854318320 @default.
- W4380794908 hasRelatedWork W2481139953 @default.
- W4380794908 hasVolume "146" @default.
- W4380794908 isParatext "false" @default.
- W4380794908 isRetracted "false" @default.
- W4380794908 workType "article" @default.