FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4380853018> ?p ?o ?g. }

• W4380853018 endingPage "3158" @default.
• W4380853018 startingPage "3148" @default.
• W4380853018 abstract "The objective of this study was to determine the role of SLC15A4 in the response to muramyl dipeptide (MDP)-mediated inflammatory response of bovine rumen epithelial cells (BRECs). First, changes in the mRNA expression of pro-inflammatory factor genes in BRECs following 10 μg mL-1 MDP treatments was examined. RT-qPCR results showed that the mRNA expressions of pro-inflammatory factors (IL-1β, IL-6, and TNF-α) were significantly increased under MDP stimulation (P < 0.001). Moreover, SLC15A4-KO cells were obtained through lentivirus packaging, transfection, screening, and cell monoclonal culture. In order to gain further insight into the potential function of SLC15A4, we utilized transcriptome data and revealed a change of genes between WT-BRECs and SLC15A4-KO. Five down-regulated pro-inflammatory genes and thirteen down-regulated chemokine genes related to the inflammatory response were identified. Meanwhile, the down-regulated genes were mostly enriched in NF-κB and MAPK signaling pathway. The results of RT-qPCR also verified these detected changes. To further determine the mechanism of how WT and SLC15A4-KO BRECs involved inflammatory responses, we investigated the inflammatory response of cells exposed to MDP. WT-BRECs and SLC15A4-KO were treated with a culture medium increase of 10 μg mL-1 MDP, in comparison to a control without MDP. Our results show that SLC15A4-KO BRECs decreased the expression of genes (IL-6, TNF-α, CXCL2, CXCL3, CXCL9, and CCL2) and proteins (p-p65 and p-p44/42) from MDP-mediated inflammatory response compared to WT-BRECs (P < 0.05). In this experiment, CRISPR-Cas9 was used to KO the di/tripeptide transporter SLC15A4, and its role was confirmed via MDP-induced inflammatory response in BRECs. This work will provide a theoretical basis for the study of the pro-inflammatory mechanism of MDP and its application in the prevention and treatment work of subacute rumen acidosis in dairy cows." @default.
• W4380853018 created "2023-06-16" @default.
• W4380853018 creator A5003947656 @default.
• W4380853018 creator A5004019041 @default.
• W4380853018 creator A5023514607 @default.
• W4380853018 creator A5028525295 @default.
• W4380853018 creator A5041927319 @default.
• W4380853018 creator A5070528488 @default.
• W4380853018 creator A5090305548 @default.
• W4380853018 date "2023-10-01" @default.
• W4380853018 modified "2023-10-10" @default.
• W4380853018 title "CRISPR/Cas9-mediated knock-out of SLC15A4 gene involved immune response in bovine rumen epithelial cells1" @default.
• W4380853018 cites W1599736469 @default.
• W4380853018 cites W1759917824 @default.
• W4380853018 cites W1969913081 @default.
• W4380853018 cites W1988347181 @default.
• W4380853018 cites W2003572328 @default.
• W4380853018 cites W2005626121 @default.
• W4380853018 cites W2031113436 @default.
• W4380853018 cites W2031211461 @default.
• W4380853018 cites W2031580483 @default.
• W4380853018 cites W2051569082 @default.
• W4380853018 cites W2053458140 @default.
• W4380853018 cites W2053833292 @default.
• W4380853018 cites W2053941125 @default.
• W4380853018 cites W2075583035 @default.
• W4380853018 cites W2079936328 @default.
• W4380853018 cites W2088120033 @default.
• W4380853018 cites W2128440643 @default.
• W4380853018 cites W2150532865 @default.
• W4380853018 cites W2151713498 @default.
• W4380853018 cites W2152976977 @default.
• W4380853018 cites W2170293791 @default.
• W4380853018 cites W2264810876 @default.
• W4380853018 cites W2294796874 @default.
• W4380853018 cites W2317211004 @default.
• W4380853018 cites W2397457481 @default.
• W4380853018 cites W2508113843 @default.
• W4380853018 cites W2611449600 @default.
• W4380853018 cites W2799522542 @default.
• W4380853018 cites W2799881301 @default.
• W4380853018 cites W2803130294 @default.
• W4380853018 cites W2891245460 @default.
• W4380853018 cites W2963842764 @default.
• W4380853018 cites W2968979230 @default.
• W4380853018 cites W3040174751 @default.
• W4380853018 cites W3046301510 @default.
• W4380853018 cites W3049765995 @default.
• W4380853018 cites W3095424764 @default.
• W4380853018 cites W4281742441 @default.
• W4380853018 doi "https://doi.org/10.1016/j.jia.2023.06.016" @default.
• W4380853018 hasPublicationYear "2023" @default.
• W4380853018 type Work @default.
• W4380853018 citedByCount "0" @default.
• W4380853018 crossrefType "journal-article" @default.
• W4380853018 hasAuthorship W4380853018A5003947656 @default.
• W4380853018 hasAuthorship W4380853018A5004019041 @default.
• W4380853018 hasAuthorship W4380853018A5023514607 @default.
• W4380853018 hasAuthorship W4380853018A5028525295 @default.
• W4380853018 hasAuthorship W4380853018A5041927319 @default.
• W4380853018 hasAuthorship W4380853018A5070528488 @default.
• W4380853018 hasAuthorship W4380853018A5090305548 @default.
• W4380853018 hasBestOaLocation W43808530181 @default.
• W4380853018 hasConcept C104317684 @default.
• W4380853018 hasConcept C150194340 @default.
• W4380853018 hasConcept C153911025 @default.
• W4380853018 hasConcept C162317418 @default.
• W4380853018 hasConcept C164027704 @default.
• W4380853018 hasConcept C17991360 @default.
• W4380853018 hasConcept C185592680 @default.
• W4380853018 hasConcept C203014093 @default.
• W4380853018 hasConcept C2776914184 @default.
• W4380853018 hasConcept C54355233 @default.
• W4380853018 hasConcept C68324877 @default.
• W4380853018 hasConcept C86803240 @default.
• W4380853018 hasConceptScore W4380853018C104317684 @default.
• W4380853018 hasConceptScore W4380853018C150194340 @default.
• W4380853018 hasConceptScore W4380853018C153911025 @default.
• W4380853018 hasConceptScore W4380853018C162317418 @default.
• W4380853018 hasConceptScore W4380853018C164027704 @default.
• W4380853018 hasConceptScore W4380853018C17991360 @default.
• W4380853018 hasConceptScore W4380853018C185592680 @default.
• W4380853018 hasConceptScore W4380853018C203014093 @default.
• W4380853018 hasConceptScore W4380853018C2776914184 @default.
• W4380853018 hasConceptScore W4380853018C54355233 @default.
• W4380853018 hasConceptScore W4380853018C68324877 @default.
• W4380853018 hasConceptScore W4380853018C86803240 @default.
• W4380853018 hasIssue "10" @default.
• W4380853018 hasLocation W43808530181 @default.
• W4380853018 hasOpenAccess W4380853018 @default.
• W4380853018 hasPrimaryLocation W43808530181 @default.
• W4380853018 hasRelatedWork W1976510917 @default.
• W4380853018 hasRelatedWork W1992848314 @default.
• W4380853018 hasRelatedWork W2015667478 @default.
• W4380853018 hasRelatedWork W2146271562 @default.
• W4380853018 hasRelatedWork W2392126385 @default.
• W4380853018 hasRelatedWork W2416795054 @default.
• W4380853018 hasRelatedWork W2944714515 @default.

• next