Matches in SemOpenAlex for { <https://semopenalex.org/work/W4380883959> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W4380883959 abstract "Abstract Background The presence of extracellular amyloid β (Aβ) plaques in the brain parenchyma and intracellular neurofibrillary tangles of the hyperphosphorylated tau protein is the main hallmark of Alzheimer’s disease (AD). Aβ aggregation consists of a complex chain of nucleation events producing soluble oligomer intermediates, which are the major neurotoxic agents. The transient nature of these oligomers makes very challenge their isolation and characterization. Single domain Abs (sdAbs), composed only of a variable domain of the heavy chain with high specificity and affinity, appear as promising tools for an early accurate diagnosis and therapy for AD. Method An in vitro and in vivo screening of different sdAbs was performed, selecting those targeting Aβ 42 oligomers with high specificity using immunoassays and the super resolution stimulated emission depletion (STED) microscopy. The ability of sdAbs to prevent Aβ 42 ‐induced cytotoxicity in neuronal cells was investigated. Then, the sdAbs were used to detect and quantify the levels of Aβ 42 assemblies in the cerebrospinal fluid (CSF) of AD patients and controls. Result SdAbs can detect neurotoxic Aβ assemblies and prevent the associated neuronal dysfunction in cell models. Furthermore, sdAbs significantly discriminated Aβ aggregates in the CSF. Conclusion All these data provide solid grounds for the understanding of the molecular basis of oligomers toxicity, thus establishing a compelling structure‐toxicity relationship. Moreover, sdAbs‐based approaches hold a great potential as they allow detection in complex mixtures such as a biological sample for an early differential diagnosis of AD, also providing therapeutic benefits. The study was supported by Airalzh (AGYR 2020 to R.C.)." @default.
- W4380883959 created "2023-06-17" @default.
- W4380883959 creator A5020167318 @default.
- W4380883959 creator A5032721511 @default.
- W4380883959 creator A5041491069 @default.
- W4380883959 creator A5078280336 @default.
- W4380883959 creator A5080762982 @default.
- W4380883959 creator A5087434482 @default.
- W4380883959 creator A5090661514 @default.
- W4380883959 date "2023-06-01" @default.
- W4380883959 modified "2023-09-27" @default.
- W4380883959 title "Single domain antibodies as new detectors of neurotoxic Aβ<sub>42</sub> oligomers" @default.
- W4380883959 doi "https://doi.org/10.1002/alz.065735" @default.
- W4380883959 hasPublicationYear "2023" @default.
- W4380883959 type Work @default.
- W4380883959 citedByCount "0" @default.
- W4380883959 crossrefType "journal-article" @default.
- W4380883959 hasAuthorship W4380883959A5020167318 @default.
- W4380883959 hasAuthorship W4380883959A5032721511 @default.
- W4380883959 hasAuthorship W4380883959A5041491069 @default.
- W4380883959 hasAuthorship W4380883959A5078280336 @default.
- W4380883959 hasAuthorship W4380883959A5080762982 @default.
- W4380883959 hasAuthorship W4380883959A5087434482 @default.
- W4380883959 hasAuthorship W4380883959A5090661514 @default.
- W4380883959 hasBestOaLocation W43808839591 @default.
- W4380883959 hasConcept C12554922 @default.
- W4380883959 hasConcept C153911025 @default.
- W4380883959 hasConcept C159654299 @default.
- W4380883959 hasConcept C185592680 @default.
- W4380883959 hasConcept C203014093 @default.
- W4380883959 hasConcept C207001950 @default.
- W4380883959 hasConcept C2777820669 @default.
- W4380883959 hasConcept C54355233 @default.
- W4380883959 hasConcept C71924100 @default.
- W4380883959 hasConcept C86803240 @default.
- W4380883959 hasConceptScore W4380883959C12554922 @default.
- W4380883959 hasConceptScore W4380883959C153911025 @default.
- W4380883959 hasConceptScore W4380883959C159654299 @default.
- W4380883959 hasConceptScore W4380883959C185592680 @default.
- W4380883959 hasConceptScore W4380883959C203014093 @default.
- W4380883959 hasConceptScore W4380883959C207001950 @default.
- W4380883959 hasConceptScore W4380883959C2777820669 @default.
- W4380883959 hasConceptScore W4380883959C54355233 @default.
- W4380883959 hasConceptScore W4380883959C71924100 @default.
- W4380883959 hasConceptScore W4380883959C86803240 @default.
- W4380883959 hasIssue "S7" @default.
- W4380883959 hasLocation W43808839591 @default.
- W4380883959 hasOpenAccess W4380883959 @default.
- W4380883959 hasPrimaryLocation W43808839591 @default.
- W4380883959 hasRelatedWork W1961082447 @default.
- W4380883959 hasRelatedWork W1969289591 @default.
- W4380883959 hasRelatedWork W2004532756 @default.
- W4380883959 hasRelatedWork W2037568211 @default.
- W4380883959 hasRelatedWork W2370862083 @default.
- W4380883959 hasRelatedWork W2380360044 @default.
- W4380883959 hasRelatedWork W2911642573 @default.
- W4380883959 hasRelatedWork W2978866030 @default.
- W4380883959 hasRelatedWork W4234120924 @default.
- W4380883959 hasRelatedWork W4298288890 @default.
- W4380883959 hasVolume "19" @default.
- W4380883959 isParatext "false" @default.
- W4380883959 isRetracted "false" @default.
- W4380883959 workType "article" @default.